Question 1

Which of the following results from inserting foreign DNA into an organism to produce a new gene combination?

Accepted Answer

A) recombinant DNA 
B) regulatory genes 
C) mutations 
D) translation 
E) gene cloning 
A) recombinant DNA 
B) regulatory genes 
C) mutations 
D) translation 
E) gene cloning

Question 2

An organism that contains foreign DNA, which was inserted into its own genetic material, is called:

Accepted Answer

A) transgenic. 
B) polygenic. 
C) electrophoresed. 
D) foreign. 
A) transgenic. 
B) polygenic. 
C) electrophoresed. 
D) foreign.

Question 3

Sexual reproduction in humans is an example of DNA recombination. True or False?

Accepted Answer

A) True 
 B)False

Question 4

________ animals and plants express the DNA inserted in them that was derived from another species.

Accepted Answer

The answer of ________ animals and plants express the DNA...

Question 5

Which of the following is the correct sequence of the polymerase chain reaction?

Accepted Answer

A) heat to 70°C (to separate DN
A) , then cool to 50°C (to bind primers and enzymes), then reheat to 90°C (to synthesize DN
A)  
B) heat to 90°C (to bind primers and enzymes), then cool to 50°C (to separate DN
A) , then reheat to 70°C (to synthesize DN
A)  
C) heat to 70°C (to bind primers and enzymes), then cool to 50°C (to synthesize DN
A) , then reheat to 90°C (to separate DN
A)  
D) heat to 90°C (to separate DN
A) , then cool to 50°C (to bind primers and enzymes), then reheat to 70°C (to synthesize DN
A)  
A) heat to 70°C (to separate DN
A) , then cool to 50°C (to bind primers and enzymes), then reheat to 90°C (to synthesize DN
A)  
B) heat to 90°C (to bind primers and enzymes), then cool to 50°C (to separate DN
A) , then reheat to 70°C (to synthesize DN
A)  
C) heat to 70°C (to bind primers and enzymes), then cool to 50°C (to synthesize DN
A) , then reheat to 90°C (to separate DN
A)  
D) heat to 90°C (to separate DN
A) , then cool to 50°C (to bind primers and enzymes), then reheat to 70°C (to synthesize DN
A)

Question 6

The polymerase chain reaction (PCR)is essential for:

Accepted Answer

A) analyzing a person's fingerprints. 
B) cutting DNA into many small pieces. 
C) allowing restriction enzymes to cut DNA. 
D) creating recombinant plasmids. 
E) making many copies of a small amount of DNA. 
A) analyzing a person's fingerprints. 
B) cutting DNA into many small pieces. 
C) allowing restriction enzymes to cut DNA. 
D) creating recombinant plasmids. 
E) making many copies of a small amount of DNA.

Question 7

In order to join a fragment of human DNA to bacterial or yeast DNA, both the human DNA and the bacterial or yeast DNA must first be treated with the same:

Accepted Answer

A) DNA ligase. 
B) DNA polymerase. 
C) restriction enzyme. 
D) DNA gyrase. 
A) DNA ligase. 
B) DNA polymerase. 
C) restriction enzyme. 
D) DNA gyrase.

Question 8

Which of the following is NOT a use for biotechnology?

Accepted Answer

A) producing large quantities of particular human proteins 
B) producing effective and safe vaccines 
C) identifying human fetuses with particular genetic diseases 
D) altering food plants to increase yields 
E) altering the intelligence levels of newborn infants 
A) producing large quantities of particular human proteins 
B) producing effective and safe vaccines 
C) identifying human fetuses with particular genetic diseases 
D) altering food plants to increase yields 
E) altering the intelligence levels of newborn infants

Question 9

In the 1990s, researchers attempted to improve the balance of amino acids in soybean protein by inserting a gene from Brazil nuts. Why have these transgenic soybeans never been grown commercially?

Accepted Answer

A) The public was misinformed by food-safety advocates and refused to buy products made from the modified soybeans. 
B) The modified soybeans could not grow with the foreign gene present. 
C) Some people were allergic to the protein product of the Brazil nut gene. 
D) Regulatory hurdles made the modified soybeans too expensive to market profitably. 
E) The protein product of the soybean was indigestible to most people. 
A) The public was misinformed by food-safety advocates and refused to buy products made from the modified soybeans. 
B) The modified soybeans could not grow with the foreign gene present. 
C) Some people were allergic to the protein product of the Brazil nut gene. 
D) Regulatory hurdles made the modified soybeans too expensive to market profitably. 
E) The protein product of the soybean was indigestible to most people.

Question 10

Imagine that it is 1965 and the bacterium Thermus aquaticus has not yet been discovered. As a geneticist interested in forensics applications, you are attempting to develop a gene-amplifying technique similar to PCR. In a test tube, you place a DNA sample, free nucleotides, human DNA polymerase, and the appropriate primers for the DNA sequence being analyzed. You first heat the sample to 90°C, then cool it to 50°C, and then reheat it to 70°C. Based on this scenario and your knowledge of how modern PCR works, the result of these procedures will be:

Accepted Answer

A) successful copying of the DNA, because it follows modern PCR procedures almost perfectly. 
B) unsuccessful, because the enzyme will denature during the first step (heating). 
C) unsuccessful, because the enzyme will denature during the second step (cooling). 
D) unsuccessful, because the enzyme will denature during the third step (reheating). 
E) unsuccessful, because human DNA polymerase cannot recognize the primers used. 
A) successful copying of the DNA, because it follows modern PCR procedures almost perfectly. 
B) unsuccessful, because the enzyme will denature during the first step (heating). 
C) unsuccessful, because the enzyme will denature during the second step (cooling). 
D) unsuccessful, because the enzyme will denature during the third step (reheating). 
E) unsuccessful, because human DNA polymerase cannot recognize the primers used.

Exam 13: Biotechnology

Which of the following results from inserting foreign DNA into an organism to produce a new gene combination?

An organism that contains foreign DNA, which was inserted into its own genetic material, is called:

Sexual reproduction in humans is an example of DNA recombination. True or False?

________ animals and plants express the DNA inserted in them that was derived from another species.

Which of the following is the correct sequence of the polymerase chain reaction?

The polymerase chain reaction (PCR)is essential for:

In order to join a fragment of human DNA to bacterial or yeast DNA, both the human DNA and the bacterial or yeast DNA must first be treated with the same:

Which of the following is NOT a use for biotechnology?

In the 1990s, researchers attempted to improve the balance of amino acids in soybean protein by inserting a gene from Brazil nuts. Why have these transgenic soybeans never been grown commercially?

An Introduction to Life on Earth

Atoms, Molecules, and Life

Biological Molecules

Cell Structure and Function

Cell Membrane Structure and Function

Energy Flow in the Life of a Cell

Capturing Solar Energy: Photosynthesis

Harvesting Energy: Glycolysis and Cellular Respiration

The Continuity of Life: Cellular Reproduction

Patterns of Inheritance

Dna: the Molecule of Heredity

Gene Expression and Regulation

Principles of Evolution

How Populations Evolve

The Origin of Species

The History of Life

Systematics: Seeking Order Amidst Diversity

The Diversity of Prokaryotes and Viruses

The Diversity of Protists

The Diversity of Plants

The Diversity of Fungi

Animal Diversity I: Invertebrates

Animal Diversity Ii: Vertebrates

Animal Behavior

Population Growth and Regulation

Community Interactions

How Do Ecosystems Work

Earths Diverse Ecosystems

Conserving Earths Biodiversity

Homeostasis and the Organization of the Animal Body

Circulation

Respiration

Nutrition and Digestion

The Urinary System

Defenses Against Disease

Chemical Control of the Animal Body: the Endocrine System

The Nervous System

The Senses

Action and Support: the Muscles and Skeleton

Animal Reproduction

Animal Development

Plant Anatomy and Nutrient Transport

Plant Reproduction and Development

Plant Responses to the Environment

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