Question 1

The system of taxonomy used today was originated by (Linnaeus/Darwin/Woese).

Accepted Answer

Linnaeus

Question 2

A resolution of 1μm would be better than a resolution of 0.5 μm.

Accepted Answer

A) True 
 B)False  False

Question 3

Three-dimensional images of specimens can be obtained using scanning electron microscopes.

Accepted Answer

A) True 
 B)False  True

Question 4

A new single-celled organism is discovered living in an ant colony. Which of the following classification methods would be appropriate for identifying to which domain it belongs?

Accepted Answer

A)  physical characteristics 
B)  biochemical tests 
C)  phage typing 
D)  analysis of nucleic acids 
E)  serological tests 
A)  physical characteristics 
B)  biochemical tests 
C)  phage typing 
D)  analysis of nucleic acids 
E)  serological tests

Question 5

A primary purpose for the use of stains in microscopy is the increase the (magnification/brightness/contrast) of a specimen.

Accepted Answer

The answer of A primary purpose for the use of...

Question 6

The rules of naming organisms are called

Accepted Answer

A)  taxonomy. 
B)  classification. 
C)  identification. 
D)  nomenclature. 
E)  binomials. 
A)  taxonomy. 
B)  classification. 
C)  identification. 
D)  nomenclature. 
E)  binomials.

Question 7

Compare and contrast the light microscope with the electron microscope.

Accepted Answer

Both the light microscope and the electr

Question 8

The technique called (confocal/electron/probe) microscopy uses detection of deflection to map surfaces of molecular structures.

Accepted Answer

The answer of The technique called (confocal/electron/probe) microscopy uses detection...

Question 9

This image was most likely produced by a microscope.

Accepted Answer

A)  scanning electron 
B)  bright-field 
C)  confocal 
D)  transmission electron 
E)  dark-field 
A)  scanning electron 
B)  bright-field 
C)  confocal 
D)  transmission electron 
E)  dark-field

Question 10

Carl Woese proposed the concept of the domain based on differences of which of the following cellular molecules?

Accepted Answer

A)  DNA 
B)  ribosomal RNA 
C)  proteins 
D)  membrane lipids 
E)  transfer RNA 
A)  DNA 
B)  ribosomal RNA 
C)  proteins 
D)  membrane lipids 
E)  transfer RNA

Question 11

Which of the following classification methods relies on the morphology of organisms?

Accepted Answer

A)  biochemical tests 
B)  physical (phenotypic) characteristics 
C)  analysis of nucleic acids 
D)  serological tests 
E)  phage typing 
A)  biochemical tests 
B)  physical (phenotypic) characteristics 
C)  analysis of nucleic acids 
D)  serological tests 
E)  phage typing

Question 12

The Gram stain works because of differences in the of bacteria.

Accepted Answer

A)  cell membranes 
B)  genetic characteristics 
C)  antigens 
D)  cell walls 
E)  capsules 
A)  cell membranes 
B)  genetic characteristics 
C)  antigens 
D)  cell walls 
E)  capsules

Question 13

The stain is one in which eosin is used.

Accepted Answer

A)  flagellar 
B)  electron microscopy 
C)  negative 
D)  endospore 
E)  acid-fast 
A)  flagellar 
B)  electron microscopy 
C)  negative 
D)  endospore 
E)  acid-fast

Question 14

Species and strains of microbes can be distinguished from one another phenotypically using

Accepted Answer

A)  Gram-stain reactions. 
B)  electron microscopy. 
C)  ribosomal RNA analysis. 
D)  serological tests. 
E)  G + C content. 
A)  Gram-stain reactions. 
B)  electron microscopy. 
C)  ribosomal RNA analysis. 
D)  serological tests. 
E)  G + C content.

Question 15

Tungsten is a reagent used in the

Accepted Answer

A)  flagellar stain. 
B)  negative stain. 
C)  endospore stain. 
D)  electron microscopy stain. 
E)  acid-fast stain. 
A)  flagellar stain. 
B)  negative stain. 
C)  endospore stain. 
D)  electron microscopy stain. 
E)  acid-fast stain.

Question 16

Why have some microbiologists proposed using ribosomal RNA as the basis for defining bacterial species?

Accepted Answer

A)  Bacteria are not interbreeding populations, and ribosomal RNAs are highly conserved genes present in all prokaryotes. 
B)  Ribosomal RNAs are highly conserved genetic sequences present in all prokaryotes. 
C)  Bacteria vary too little in their physical and biochemical traits. 
D)  The ʺinterbreeding populationʺ criterion does not apply to bacteria. 
E)  Ribosomal RNA is the basis for domain assignment. 
A)  Bacteria are not interbreeding populations, and ribosomal RNAs are highly conserved genes present in all prokaryotes. 
B)  Ribosomal RNAs are highly conserved genetic sequences present in all prokaryotes. 
C)  Bacteria vary too little in their physical and biochemical traits. 
D)  The ʺinterbreeding populationʺ criterion does not apply to bacteria. 
E)  Ribosomal RNA is the basis for domain assignment.

Question 17

The agglutination test uses proteins called (antibodies/antigens/chromophores) to detect species-specific features of microbes.

Accepted Answer

The answer of The agglutination test uses proteins called (antibodies/antigens/chromophores)...

Question 18

Acidic dyes

Accepted Answer

A)  are used for staining negatively charged molecular structures. 
B)  are negatively charged and work best at low pH. 
C)  are lipid soluble. 
D)  are negatively charged. 
E)  work best in low-pH environments. 
A)  are used for staining negatively charged molecular structures. 
B)  are negatively charged and work best at low pH. 
C)  are lipid soluble. 
D)  are negatively charged. 
E)  work best in low-pH environments.

Question 19

The ability of a lens to gather light is referred to as its

Accepted Answer

A)  numerical aperture. 
B)  magnification. 
C)  contrast. 
D)  resolution. 
E)  refraction. 
A)  numerical aperture. 
B)  magnification. 
C)  contrast. 
D)  resolution. 
E)  refraction.

Question 20

Safranin dye is used as the counterstain in stain(s).

Accepted Answer

A)  the endospore 
B)  the acid-fast 
C)  the flagellar 
D)  the Gram 
E)  both the Gram and the endospore 
A)  the endospore 
B)  the acid-fast 
C)  the flagellar 
D)  the Gram 
E)  both the Gram and the endospore

Exam 4: Microscopy, Staining, and Classification

The system of taxonomy used today was originated by (Linnaeus/Darwin/Woese).

A resolution of 1μm would be better than a resolution of 0.5 μm.

Three-dimensional images of specimens can be obtained using scanning electron microscopes.

A new single-celled organism is discovered living in an ant colony. Which of the following classification methods would be appropriate for identifying to which domain it belongs?

A primary purpose for the use of stains in microscopy is the increase the (magnification/brightness/contrast) of a specimen.

The rules of naming organisms are called

Compare and contrast the light microscope with the electron microscope.

The technique called (confocal/electron/probe) microscopy uses detection of deflection to map surfaces of molecular structures.

This image was most likely produced by a microscope.

Carl Woese proposed the concept of the domain based on differences of which of the following cellular molecules?

Which of the following classification methods relies on the morphology of organisms?

The Gram stain works because of differences in the of bacteria.

The stain is one in which eosin is used.

Species and strains of microbes can be distinguished from one another phenotypically using

Tungsten is a reagent used in the

Why have some microbiologists proposed using ribosomal RNA as the basis for defining bacterial species?

The agglutination test uses proteins called (antibodies/antigens/chromophores) to detect species-specific features of microbes.

Acidic dyes

The ability of a lens to gather light is referred to as its

Safranin dye is used as the counterstain in stain(s).

A Brief History of Microbiology

The Chemistry of Microbiology

Cell Structure and Function

Microbial Metabolism

Microbial Nutrition and Growth

Microbial Genetics

Recombinant Dna Technology

Controlling Microbial Growth in the Environment

Controlling Microbial Growth in the Body: Antimicrobial Drugs

Characterizing and Classifying Prokaryotes

Characterizing and Classifying Eukaryotes

Characterizing and Classifying Viruses, Viroids, and Prions

Infection, Infectious Diseases, and Epidemiology

Innate Immunity

Adaptive Immunity

Immunization and Immune Testing

Aids and Other Immune Disorders

Microbial Diseases of the Skin and Wounds

Microbial Diseases of the Nervous System and Eyes

Microbial Cardiovascular and Systemic Diseases

Microbial Diseases of the Respiratory System

Microbial Diseases of the Digestive System

Microbial Diseases of the Urinary and Reproductive Systems

Applied and Environmental Microbiology

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