Question 1

The number of DNA molecules produced during PCR increases exponentially.

Accepted Answer

A) True 
 B)False

Question 2

, the separation of double- stranded DNA molecules, is generally accomplished by heating the molecules to near- boiling temperatures.

Accepted Answer

The answer of , the separation of double- stranded DNA...

Question 3

All of the following are applications of transgenic organisms EXCEPT:

Accepted Answer

A)  tomato plants that contain a gene for salt tolerance. 
B)  "Roundup ready" soybeans and cotton. 
C)  bacteria engineered to prevent ice crystals from forming on crops. 
D)  crops that produce Bt toxin. 
E)  human hearts repaired with pig heart valves. 
A)  tomato plants that contain a gene for salt tolerance. 
B)  "Roundup ready" soybeans and cotton. 
C)  bacteria engineered to prevent ice crystals from forming on crops. 
D)  crops that produce Bt toxin. 
E)  human hearts repaired with pig heart valves.

Question 4

Short nucleic acid molecules used to locate complementary sequences in a larger population of molecules are called .

Accepted Answer

The answer of Short nucleic acid molecules used to locate...

Question 5

Which of the following is a disadvantage of using plasmids as vectors?

Accepted Answer

A)  They frequently carry undesirable genes such as those for antibiotic resistance. 
B)  They cannot be used for genetic expression. 
C)  They are not very stable in cells. 
D)  The genes they carry have to be relatively small. 
E)  They are hard to introduce into cells. 
A)  They frequently carry undesirable genes such as those for antibiotic resistance. 
B)  They cannot be used for genetic expression. 
C)  They are not very stable in cells. 
D)  The genes they carry have to be relatively small. 
E)  They are hard to introduce into cells.

Question 6

Circular DNA molecules are generally less stable inside cells than are linear molecules.

Accepted Answer

A) True 
 B)False

Question 7

All of the following are possible applications of DNA fingerprinting EXCEPT:

Accepted Answer

A)  identifying human remains. 
B)  identifying pathogens. 
C)  linking crime scene specimens to suspects. 
D)  replacing defective genes with normal copies. 
E)  paternity testing. 
A)  identifying human remains. 
B)  identifying pathogens. 
C)  linking crime scene specimens to suspects. 
D)  replacing defective genes with normal copies. 
E)  paternity testing.

Question 8

Reverse transcriptase synthesizes an RNA molecule from a DNA template.

Accepted Answer

A) True 
 B)False

Question 9

Gene therapy for human genetic diseases has not been successful yet.

Accepted Answer

A) True 
 B)False

Question 10

A(n) is a collection of bacterial or phage clones, each of which contains a portion of a genetic region of interest.

Accepted Answer

The answer of A(n) is a collection of bacterial or...

Question 11

The success of genetic engineering is due to enzymes and processes derived from all of the following EXCEPT:

Accepted Answer

A)  chemiosmosis. 
B)  transformation. 
C)  transcription. 
D)  conjugation. 
E)  transduction. 
A)  chemiosmosis. 
B)  transformation. 
C)  transcription. 
D)  conjugation. 
E)  transduction.

Question 12

is the use of microbes to make practical products such as vaccines or hormones.

Accepted Answer

The answer of is the use of microbes to make...

Question 13

What is the significance of the microbe Deinococcus radiodurans?

Accepted Answer

A)  It is the source of a wide variety of restriction enzymes. 
B)  It may provide clues to methods of reversing genetic damage in many cancer patients. 
C)  It can be engineered as a highly efficient vaccine vector. 
D)  It is now known to be a major source of greenhouse gases such as methane. 
E)  It has provided many of the molecular tools that make recombinant DNA technology possible. 
A)  It is the source of a wide variety of restriction enzymes. 
B)  It may provide clues to methods of reversing genetic damage in many cancer patients. 
C)  It can be engineered as a highly efficient vaccine vector. 
D)  It is now known to be a major source of greenhouse gases such as methane. 
E)  It has provided many of the molecular tools that make recombinant DNA technology possible.

Question 14

The is a technique used to detect specific RNA molecules in a larger population of molecules that has been separated by gel electrophoresis.

Accepted Answer

The answer of The is a technique used to detect...

Question 15

What is reverse transcriptase, and why is it an important tool of recombinant DNA technology?

Accepted Answer

Reverse transcriptase is an enzyme produ

Question 16

Compare and contrast the two major types of restriction enzymes.

Accepted Answer

All restriction enzymes recognize specif

Question 17

Protoplasts are associated with which of the following?

Accepted Answer

A)  xenotransplants 
B)  electroporation 
C)  PCR 
D)  gene therapy 
E)  Southern blotting 
A)  xenotransplants 
B)  electroporation 
C)  PCR 
D)  gene therapy 
E)  Southern blotting

Question 18

The role of restriction enzymes in bacteria is

Accepted Answer

A)  to make conjugation more efficient. 
B)  to provide the cell with new phenotypes, such as antibiotic resistance. 
C)  to protect the cell from invading phages. 
D)  to allow transposons to move to another place in the chromosome. 
E)  to allow cells to accept foreign DNA. 
A)  to make conjugation more efficient. 
B)  to provide the cell with new phenotypes, such as antibiotic resistance. 
C)  to protect the cell from invading phages. 
D)  to allow transposons to move to another place in the chromosome. 
E)  to allow cells to accept foreign DNA.

Question 19

Nucleic acid molecules used to deliver new genes to cells are called .

Accepted Answer

The answer of Nucleic acid molecules used to deliver new...

Question 20

All of the following are required to generate a cDNA library EXCEPT:

Accepted Answer

A)  mRNA. 
B)  reverse transcriptase. 
C)  restriction enzyme. 
D)  DNA primer. 
E)  a vector. 
A)  mRNA. 
B)  reverse transcriptase. 
C)  restriction enzyme. 
D)  DNA primer. 
E)  a vector.

Exam 8: Recombinant Dna Technology

The number of DNA molecules produced during PCR increases exponentially.

, the separation of double- stranded DNA molecules, is generally accomplished by heating the molecules to near- boiling temperatures.

All of the following are applications of transgenic organisms EXCEPT:

Short nucleic acid molecules used to locate complementary sequences in a larger population of molecules are called .

Which of the following is a disadvantage of using plasmids as vectors?

Circular DNA molecules are generally less stable inside cells than are linear molecules.

All of the following are possible applications of DNA fingerprinting EXCEPT:

Reverse transcriptase synthesizes an RNA molecule from a DNA template.

Gene therapy for human genetic diseases has not been successful yet.

A(n) is a collection of bacterial or phage clones, each of which contains a portion of a genetic region of interest.

The success of genetic engineering is due to enzymes and processes derived from all of the following EXCEPT:

is the use of microbes to make practical products such as vaccines or hormones.

What is the significance of the microbe Deinococcus radiodurans?

The is a technique used to detect specific RNA molecules in a larger population of molecules that has been separated by gel electrophoresis.

What is reverse transcriptase, and why is it an important tool of recombinant DNA technology?

Compare and contrast the two major types of restriction enzymes.

Protoplasts are associated with which of the following?

The role of restriction enzymes in bacteria is

Nucleic acid molecules used to deliver new genes to cells are called .

All of the following are required to generate a cDNA library EXCEPT:

A Brief History of Microbiology

The Chemistry of Microbiology

Cell Structure and Function

Microscopy, Staining, and Classification

Microbial Metabolism

Microbial Nutrition and Growth

Microbial Genetics

Controlling Microbial Growth in the Environment

Controlling Microbial Growth in the Body: Antimicrobial Drugs

Characterizing and Classifying Prokaryotes

Characterizing and Classifying Eukaryotes

Characterizing and Classifying Viruses, Viroids, and Prions

Infection, Infectious Diseases, and Epidemiology

Innate Immunity

Adaptive Immunity

Immunization and Immune Testing

Aids and Other Immune Disorders

Microbial Diseases of the Skin and Wounds

Microbial Diseases of the Nervous System and Eyes

Microbial Cardiovascular and Systemic Diseases

Microbial Diseases of the Respiratory System

Microbial Diseases of the Digestive System

Microbial Diseases of the Urinary and Reproductive Systems

Applied and Environmental Microbiology

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