Question 1

consist of single- stranded DNA immobilized on glass slides, silicon chips, or nylon membranes.

Accepted Answer

The answer of consist of single- stranded DNA immobilized on...

Question 2

The Latin term meaning "within a cell" or "in life" is .

Accepted Answer

The answer of The Latin term meaning "within a cell"...

Question 3

Subunit vaccines are safer than traditional vaccines because

Accepted Answer

A)  they are not at risk for carrying the disease. 
B)  they are acellular. 
C)  they are administered in food. 
D)  Both A and C are correct. 
E)  A, B, and C are correct. 
A)  they are not at risk for carrying the disease. 
B)  they are acellular. 
C)  they are administered in food. 
D)  Both A and C are correct. 
E)  A, B, and C are correct.

Question 4

Compare and contrast the types of vectors used in recombinant DNA technology.

Accepted Answer

All vectors have certain things in commo

Question 5

Any foreign DNA inserted into a cell will be inherited by that cell's progeny.

Accepted Answer

A) True 
 B)False

Question 6

The process of introducing animal organs into the human body is

Accepted Answer

A)  biotechnology. 
B)  protoplast fusion. 
C)  electrophoresis. 
D)  xenotransplantation. 
E)  recombinant DNA technology. 
A)  biotechnology. 
B)  protoplast fusion. 
C)  electrophoresis. 
D)  xenotransplantation. 
E)  recombinant DNA technology.

Question 7

What is the significance of the microbe H. influenzae in genetic technology?

Accepted Answer

A)  It was the first microbe to have its genome completely sequenced. 
B)  It produces a protein that can serve as a natural insecticide. 
C)  It is the source of the DNA polymerase used in PCR. 
D)  It was the first cellular microbe to have its entire gene map completed. 
E)  It is an excellent vector for inserting genes into human cells. 
A)  It was the first microbe to have its genome completely sequenced. 
B)  It produces a protein that can serve as a natural insecticide. 
C)  It is the source of the DNA polymerase used in PCR. 
D)  It was the first cellular microbe to have its entire gene map completed. 
E)  It is an excellent vector for inserting genes into human cells.

Question 8

Which of the following procedures might be used to detect the presence of a virus's genetic sequences in a patient's blood?

Accepted Answer

A)  electroporation 
B)  Southern blotting 
C)  genome mapping 
D)  xenotransplantation 
E)  creation of a gene library 
A)  electroporation 
B)  Southern blotting 
C)  genome mapping 
D)  xenotransplantation 
E)  creation of a gene library

Question 9

Protoplast fusion is enhanced by the presence of polyethylene glycol.

Accepted Answer

A) True 
 B)False

Question 10

Genomics is

Accepted Answer

A)  the sequencing and analysis of an organism's genetic information. 
B)  the process of identifying organisms by their unique DNA sequences. 
C)  the process of locating genes on a DNA molecule. 
D)  the process of altering organisms by the addition of genes from other organisms. 
E)  the replacement of missing or defective genes with normal copies. 
A)  the sequencing and analysis of an organism's genetic information. 
B)  the process of identifying organisms by their unique DNA sequences. 
C)  the process of locating genes on a DNA molecule. 
D)  the process of altering organisms by the addition of genes from other organisms. 
E)  the replacement of missing or defective genes with normal copies.

Question 11

Agarose

Accepted Answer

A)  is used in gel electrophoresis. 
B)  acts as a molecular sieve. 
C)  is a purified sugar component of agar. 
D)  Both B and C are correct. 
E)  A, B, and C are correct. 
A)  is used in gel electrophoresis. 
B)  acts as a molecular sieve. 
C)  is a purified sugar component of agar. 
D)  Both B and C are correct. 
E)  A, B, and C are correct.

Question 12

Which of the following microbes has been genetically engineered to contain a protein that helps protect crops from freezing?

Accepted Answer

A)  Bacillus thuringiensis 
B)  Salmonella 
C)  Pseudomonas 
D)  E. coli 
E)  Deinococcus radiodurans 
A)  Bacillus thuringiensis 
B)  Salmonella 
C)  Pseudomonas 
D)  E. coli 
E)  Deinococcus radiodurans

Question 13

Put the following steps of the production of a recombinant vector, containing a human gene, in order:
I. Introduce the recombinant plasmid into a bacterial cell.
II. Isolate the human gene and the vector DNA using restriction enzymes.
III. Ligate the DNA fragments to produce a recombinant plasmid.
IV. Grow bacterial cells on a medium containing a selective agent (such as an antibiotic).

Accepted Answer

A)  III, I, IV, II 
B)  I, III, IV, II 
C)  II, I, III, IV 
D)  II, III, I, IV 
E)  III, I, II, IV 
A)  III, I, IV, II 
B)  I, III, IV, II 
C)  II, I, III, IV 
D)  II, III, I, IV 
E)  III, I, II, IV

Question 14

If all of the following DNA fragments were analyzed on an electrophoresis gel, which one would migrate the slowest?

Accepted Answer

A)  250 base pairs 
B)  2500 base pairs 
C)  5000 base pairs 
D)  1000 base pairs 
E)  750 base pairs 
A)  250 base pairs 
B)  2500 base pairs 
C)  5000 base pairs 
D)  1000 base pairs 
E)  750 base pairs

Question 15

The process of locating genes within the nucleic acid of an organism is called .

Accepted Answer

The answer of The process of locating genes within the...

Question 16

uses an electrical current to puncture holes in a cell's membrane so that DNA can be inserted.

Accepted Answer

The answer of uses an electrical current to puncture holes...

Question 17

Which of the following would be an appropriate temperature for the second step of PCR?

Accepted Answer

A)  100°C 
B)  94°C 
C)  65°C 
D)  37°C 
E)  72°C 
A)  100°C 
B)  94°C 
C)  65°C 
D)  37°C 
E)  72°C

Question 18

molecules have sequences that bind to mRNA and genes to affect their function.

Accepted Answer

The answer of molecules have sequences that bind to mRNA...

Question 19

involves the insertion of foreign DNA directly into a cell's nucleus using a glass micropipet.

Accepted Answer

The answer of involves the insertion of foreign DNA directly...

Question 20

HIV is being considered as a vector for use in humans because

Accepted Answer

A)  it infects only human cells. 
B)  it inserts itself into human chromosomes. 
C)  it is cleaved by restriction enzymes. 
D)  it has a rapid generation time. 
E)  it kills only human cells. 
A)  it infects only human cells. 
B)  it inserts itself into human chromosomes. 
C)  it is cleaved by restriction enzymes. 
D)  it has a rapid generation time. 
E)  it kills only human cells.

Exam 8: Recombinant Dna Technology

consist of single- stranded DNA immobilized on glass slides, silicon chips, or nylon membranes.

The Latin term meaning "within a cell" or "in life" is .

Subunit vaccines are safer than traditional vaccines because

Compare and contrast the types of vectors used in recombinant DNA technology.

Any foreign DNA inserted into a cell will be inherited by that cell's progeny.

The process of introducing animal organs into the human body is

What is the significance of the microbe H. influenzae in genetic technology?

Which of the following procedures might be used to detect the presence of a virus's genetic sequences in a patient's blood?

Protoplast fusion is enhanced by the presence of polyethylene glycol.

Genomics is

Agarose

Which of the following microbes has been genetically engineered to contain a protein that helps protect crops from freezing?

If all of the following DNA fragments were analyzed on an electrophoresis gel, which one would migrate the slowest?

The process of locating genes within the nucleic acid of an organism is called .

uses an electrical current to puncture holes in a cell's membrane so that DNA can be inserted.

Which of the following would be an appropriate temperature for the second step of PCR?

molecules have sequences that bind to mRNA and genes to affect their function.

involves the insertion of foreign DNA directly into a cell's nucleus using a glass micropipet.

HIV is being considered as a vector for use in humans because

A Brief History of Microbiology

The Chemistry of Microbiology

Cell Structure and Function

Microscopy, Staining, and Classification

Microbial Metabolism

Microbial Nutrition and Growth

Microbial Genetics

Controlling Microbial Growth in the Environment

Controlling Microbial Growth in the Body: Antimicrobial Drugs

Characterizing and Classifying Prokaryotes

Characterizing and Classifying Eukaryotes

Characterizing and Classifying Viruses, Viroids, and Prions

Infection, Infectious Diseases, and Epidemiology

Innate Immunity

Adaptive Immunity

Immunization and Immune Testing

Aids and Other Immune Disorders

Microbial Diseases of the Skin and Wounds

Microbial Diseases of the Nervous System and Eyes

Microbial Cardiovascular and Systemic Diseases

Microbial Diseases of the Respiratory System

Microbial Diseases of the Digestive System

Microbial Diseases of the Urinary and Reproductive Systems

Applied and Environmental Microbiology

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