Question 1

Genetic distances associated with genetic maps are generally obtained

Accepted Answer

A)  by determining the number of chromosomes in the genome. 
B)  by assessing the location of chromosomes in the nucleus. 
C)  by an analysis of the frequency of recombination between genes. 
D)  by counting the number of base pairs between restriction sites. 
E)  by sequencing the genome. 
A)  by determining the number of chromosomes in the genome. 
B)  by assessing the location of chromosomes in the nucleus. 
C)  by an analysis of the frequency of recombination between genes. 
D)  by counting the number of base pairs between restriction sites. 
E)  by sequencing the genome.

Question 2

Announcement of the first draft sequence of the complete human genome was a monumental moment in the history of genetics. This announcement was made

Accepted Answer

A)  April 2003. 
B)  February 1997. 
C)  February 2001. 
D)  April 1953. 
E)  August 2000. 
A)  April 2003. 
B)  February 1997. 
C)  February 2001. 
D)  April 1953. 
E)  August 2000.

Question 3

Sequencing involves PCR reactions using Taq polymerase, which we learned in the previous chapter can introduce errors because it lacks a mismatch repair mechanism. What precaution can be taken in genomic sequencing projects, or other sequencing projects, to minimize the possibility of erroneous sequence?

Accepted Answer

The most common precaution tak

Question 4

Genome analysis has confirmed findings based on DNA sequences of single genes that prokaryotes are divided into two great lineages: the eubacteria and archaea. The latter may be more closely related to the Eukarya. Recount the lines of evidence these statements are based on.

Accepted Answer

Sequence divergence between archaea and

Question 5

Explain the basic differences in goal and approach of functional, structural, and comparative genomics.

Accepted Answer

Structural genomics considers physical a

Question 6

List the steps involved in constructing a knock-out mutant in yeast.

Accepted Answer

Most commonly, a "deletion module" is co

Question 7

Expressed sequence tags, or ESTs,

Accepted Answer

A)  are genetic markers generated from cDNA. 
B)  oligonucleotides derived from mRNA. 
C)  pertain to expressed genes only. 
D)  A and C 
E)  A, B, and C 
A)  are genetic markers generated from cDNA. 
B)  oligonucleotides derived from mRNA. 
C)  pertain to expressed genes only. 
D)  A and C 
E)  A, B, and C

Question 8

Which of the following sequences is considered an STR?

Accepted Answer

A)  TACGTACGTACG 
B)  TATTACGCCG 
C)  CGTATTATGC 
D)  CCCCCGGGGG 
E)  A and B 
A)  TACGTACGTACG 
B)  TATTACGCCG 
C)  CGTATTATGC 
D)  CCCCCGGGGG 
E)  A and B

Question 9

Clone contigs are a set of contiguous, partially overlapping sequences that collectively cover a whole chromosome or chromosome region without gaps.

Accepted Answer

A) True 
 B)False

Question 10

Highly variable (polymorphic) short tandem repeats are considered the best kind of markers for constructing sequence tagged site (STS) maps. Why?

Accepted Answer

Recombination can make it diff

Question 11

The first eukaryote to have its genome completely sequenced is a fungus.

Accepted Answer

A) True 
 B)False

Question 12

"Knock-out" mutants involve eliminating gene activity, a useful strategy to see what effect the gene has in physiology, development, etc.

Accepted Answer

A) True 
 B)False

Question 13

A key issue being considered by the bioethics program ELSI is

Accepted Answer

A)  the fair use of genetic information. 
B)  maintaining the privacy of genetic information. 
C)  effective use of genetic information in a clinical setting. 
D)  professional and public education. 
E)  all of the above. 
A)  the fair use of genetic information. 
B)  maintaining the privacy of genetic information. 
C)  effective use of genetic information in a clinical setting. 
D)  professional and public education. 
E)  all of the above.

Question 14

In a sequenced genome, candidate protein-encoding genes are identified by searching for

Accepted Answer

A)  ESTs. 
B)  ORFs. 
C)  SNPs. 
D)  STRs. 
E)  RFLPs. 
A)  ESTs. 
B)  ORFs. 
C)  SNPs. 
D)  STRs. 
E)  RFLPs.

Question 15

In a clone-contig mapping experiment, four clones were obtained with the following STS markers: Clone 1: O P G E Clone 2: A C D M Clone 3: X O P Clone 4: D M N X O The order of the markers in this part of the genome is:

Accepted Answer

A)  X O P A C G E D M N. 
B)  O P G E A C D M X P. 
C)  A C D M N X O P G E. 
D)  A C D M O P G E N X. 
E)  D M N X O A C D P G E. 
A)  X O P A C G E D M N. 
B)  O P G E A C D M X P. 
C)  A C D M N X O P G E. 
D)  A C D M O P G E N X. 
E)  D M N X O A C D P G E.

Question 16

The human genome contains approximately ________ genes.

Accepted Answer

A)  10,000 
B)  20,000 
C)  40,000 
D)  100,000 
E)  80,000 
A)  10,000 
B)  20,000 
C)  40,000 
D)  100,000 
E)  80,000

Question 17

Genome projects are usually justified by scientists to the public on the basis of providing knowledge of (and potential cures for) human disease. Do you think this argument is necessary? Do genome projects have to be justified in terms of curing human disease? Is the promise too ambitious?

Accepted Answer

While taxpayers and legislators may ofte

Question 18

Which of the following statements about genomes is false?

Accepted Answer

A)  Genes make up most of the DNA of prokaryotes. 
B)  The human genome contains several genes of bacterial origin. 
C)  Most of the human genome consists of coding DNA. 
D)  Eukaryotes generally have far lower gene density than prokaryotes. 
E)  Genomes show a general trend of increasing complexity with complexity of organism. 
A)  Genes make up most of the DNA of prokaryotes. 
B)  The human genome contains several genes of bacterial origin. 
C)  Most of the human genome consists of coding DNA. 
D)  Eukaryotes generally have far lower gene density than prokaryotes. 
E)  Genomes show a general trend of increasing complexity with complexity of organism.

Question 19

Which type of cloning vector is commonly used in the construction of contig maps?

Accepted Answer

A)  Plasmids 
B)  BACs 
C)  Viruses 
D)  YACs 
E)  B and D 
A)  Plasmids 
B)  BACs 
C)  Viruses 
D)  YACs 
E)  B and D

Question 20

Describe the basic approach to shotgun genome sequencing.

Accepted Answer

Shotgun sequencing involves cutting the

Exam 18: Recombinant DNA Technology

Genetic distances associated with genetic maps are generally obtained

Announcement of the first draft sequence of the complete human genome was a monumental moment in the history of genetics. This announcement was made

Genome analysis has confirmed findings based on DNA sequences of single genes that prokaryotes are divided into two great lineages: the eubacteria and archaea. The latter may be more closely related to the Eukarya. Recount the lines of evidence these statements are based on.

Explain the basic differences in goal and approach of functional, structural, and comparative genomics.

List the steps involved in constructing a knock-out mutant in yeast.

Expressed sequence tags, or ESTs,

Which of the following sequences is considered an STR?

Clone contigs are a set of contiguous, partially overlapping sequences that collectively cover a whole chromosome or chromosome region without gaps.

Highly variable (polymorphic) short tandem repeats are considered the best kind of markers for constructing sequence tagged site (STS) maps. Why?

The first eukaryote to have its genome completely sequenced is a fungus.

"Knock-out" mutants involve eliminating gene activity, a useful strategy to see what effect the gene has in physiology, development, etc.

A key issue being considered by the bioethics program ELSI is

In a sequenced genome, candidate protein-encoding genes are identified by searching for

In a clone-contig mapping experiment, four clones were obtained with the following STS markers: Clone 1: O P G E Clone 2: A C D M Clone 3: X O P Clone 4: D M N X O The order of the markers in this part of the genome is:

The human genome contains approximately ________ genes.

Genome projects are usually justified by scientists to the public on the basis of providing knowledge of (and potential cures for) human disease. Do you think this argument is necessary? Do genome projects have to be justified in terms of curing human disease? Is the promise too ambitious?

Which of the following statements about genomes is false?

Which type of cloning vector is commonly used in the construction of contig maps?

Describe the basic approach to shotgun genome sequencing.

Genetics of Bacteria and Bacteriophages

Variations in Chromosome Structure and Number

Advanced Gene Mapping in Eukaryotes

Gene Mapping in Eukaryotes

Quantitative Genetics

Extensions of Mendelian Genetic Principles

Chromosomal Basis of Inheritance

Molecular Evolution

Population Genetics

Non-Mendelian Inheritance

Genetics of Cancer

Genetic Analysis of Development

Regulation of Gene Expression in Eukaryotes

Mendelian Genetics

Regulation of Gene Expression in Bacteria and Bacteriophages

Genomics

Applications of Recombinant DNA Technology

DNA Mutation, DNA Repair, and Transposable Elements

Gene Expression: Translation

Gene Expression: Transcription

Gene Control of Proteins

DNA Replication

DNA: The Genetic Material

Genetics: an Introduction

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