Question 1

Which event occurs at the termination of transcription?

Accepted Answer

A)  The RNA chain is released from the DNA. 
B)  RNA polymerase is released from the DNA. 
C)  RNA synthesis stops. 
D)  Both A and C 
E)  All of the above 
A)  The RNA chain is released from the DNA. 
B)  RNA polymerase is released from the DNA. 
C)  RNA synthesis stops. 
D)  Both A and C 
E)  All of the above

Question 2

The function of a ribosome is

Accepted Answer

A)  to hold mRNA and tRNAs in the correct positions to enable translation. 
B)  to attach amino acids onto the appropriate tRNAs. 
C)  to make more mRNA. 
D)  to process pre-mRNAs into mature mRNAs. 
E)  to direct transcription. 
A)  to hold mRNA and tRNAs in the correct positions to enable translation. 
B)  to attach amino acids onto the appropriate tRNAs. 
C)  to make more mRNA. 
D)  to process pre-mRNAs into mature mRNAs. 
E)  to direct transcription.

Question 3

The sequence of bases of a molecule of mRNA is converted into a corresponding amino acid sequence during

Accepted Answer

A)  transformation. 
B)  translation. 
C)  replication. 
D)  transversion. 
E)  transcription. 
A)  transformation. 
B)  translation. 
C)  replication. 
D)  transversion. 
E)  transcription.

Question 4

During the initiation step of transcription, what molecule binds the promoter region of a DNA molecule?

Accepted Answer

A)  DNA polymerase 
B)  Topoisomerase 
C)  DNA primase 
D)  RNA polymerase 
E)  Reverse transcriptase 
A)  DNA polymerase 
B)  Topoisomerase 
C)  DNA primase 
D)  RNA polymerase 
E)  Reverse transcriptase

Question 5

What is the difference between an intervening sequence and an untranslated region in a eukaryotic mRNA?

Accepted Answer

Untranslated regions (UTRs) are found on

Question 6

Molecules of tRNA are

Accepted Answer

A)  cloverleaf in shape. 
B)  synthesized with modified bases. 
C)  single-stranded with secondary structure. 
D)  75 to 90 nucleotides in length. 
E)  all of the above. 
A)  cloverleaf in shape. 
B)  synthesized with modified bases. 
C)  single-stranded with secondary structure. 
D)  75 to 90 nucleotides in length. 
E)  all of the above.

Question 7

What are the three regions of a prokaryotic gene?

Accepted Answer

A promoter region, a

Question 8

Outline the basic differences in function among RNA polymerases in both prokaryotes and eukaryotes.

Accepted Answer

Only one type of RNA polymerase is found

Question 9

Which of the following is not found in a eukaryotic promoter?

Accepted Answer

A)  -10 box 
B)  TATA box 
C)  CAAT box 
D)  GC box 
E)  None of these are found in a eukaryotic promoter. 
A)  -10 box 
B)  TATA box 
C)  CAAT box 
D)  GC box 
E)  None of these are found in a eukaryotic promoter.

Question 10

Which of the following is a characteristic of a rho-dependent terminator?

Accepted Answer

A)  Rich in AT nucleotides 
B)  Hairpin loop 
C)  Termination requires ATP 
D)  Both A and B 
E)  All of the above 
A)  Rich in AT nucleotides 
B)  Hairpin loop 
C)  Termination requires ATP 
D)  Both A and B 
E)  All of the above

Question 11

Which parts of a eukaryotic gene are transcribed?

Accepted Answer

A)  Both exons and introns 
B)  Only the exons 
C)  Only the introns 
D)  Exons, introns, promoter, and terminator sequence 
E)  It depends on the gene. 
A)  Both exons and introns 
B)  Only the exons 
C)  Only the introns 
D)  Exons, introns, promoter, and terminator sequence 
E)  It depends on the gene.

Question 12

What is the function of the inverted repeat in the termination sequence of a rho-independent terminator? What would happen if a mutation occurred in this region?

Accepted Answer

RNA polymerase transcribes the inverted

Question 13

What happens during the elongation step of transcription?

Accepted Answer

A)  An RNA transcript is formed and stays bound to the DNA template. 
B)  RNA polymerase loosely binds the -35 region of the promoter and unwinds the DNA. 
C)  The sigma factor dissociates from the core enzyme. 
D)  An RNA transcript if formed and then drops off the DNA template. 
E)  A polypeptide chain is formed. 
A)  An RNA transcript is formed and stays bound to the DNA template. 
B)  RNA polymerase loosely binds the -35 region of the promoter and unwinds the DNA. 
C)  The sigma factor dissociates from the core enzyme. 
D)  An RNA transcript if formed and then drops off the DNA template. 
E)  A polypeptide chain is formed.

Question 14

What would be the advantage of having multiple sigma factors that each could be produced under different stress conditions in E. coli?

Accepted Answer

Different sigma factors recognize differ

Question 15

All genes encode proteins.

Accepted Answer

A) True 
 B)False

Question 16

RNA polymerase has no proofreading activity during transcription.

Accepted Answer

A) True 
 B)False

Question 17

The single strand of mRNA that is produced during transcription has the same polarity as the ________ strand of DNA.

Accepted Answer

A)  nonsense 
B)  template 
C)  leading 
D)  nontemplate 
E)  lagging 
A)  nonsense 
B)  template 
C)  leading 
D)  nontemplate 
E)  lagging

Question 18

What kind of experiment or analysis could you conduct in order to delineate the promoter elements of protein-coding genes?

Accepted Answer

You could examine the effect of mutation

Question 19

The sigma factor of RNA polymerase is necessary for

Accepted Answer

A)  maximum transcription of the gene. 
B)  elongation of the mRNA chain. 
C)  termination of the mRNA chain. 
D)  specific recognition of promoter elements. 
E)  formation of the transcription bubble. 
A)  maximum transcription of the gene. 
B)  elongation of the mRNA chain. 
C)  termination of the mRNA chain. 
D)  specific recognition of promoter elements. 
E)  formation of the transcription bubble.

Question 20

A prokaryotic mRNA transcript

Accepted Answer

A)  must be further processed before it is transcribed. 
B)  must be transported from the nucleus before it is transcribed. 
C)  Both A and B 
D)  undergoes coupling of transcription and translation. 
E)  is ready to be translated as soon as it is transcribed. 
A)  must be further processed before it is transcribed. 
B)  must be transported from the nucleus before it is transcribed. 
C)  Both A and B 
D)  undergoes coupling of transcription and translation. 
E)  is ready to be translated as soon as it is transcribed.

Exam 12: Genetic Analysis of Development

Which event occurs at the termination of transcription?

The function of a ribosome is

The sequence of bases of a molecule of mRNA is converted into a corresponding amino acid sequence during

During the initiation step of transcription, what molecule binds the promoter region of a DNA molecule?

What is the difference between an intervening sequence and an untranslated region in a eukaryotic mRNA?

Molecules of tRNA are

What are the three regions of a prokaryotic gene?

Outline the basic differences in function among RNA polymerases in both prokaryotes and eukaryotes.

Which of the following is not found in a eukaryotic promoter?

Which of the following is a characteristic of a rho-dependent terminator?

Which parts of a eukaryotic gene are transcribed?

What is the function of the inverted repeat in the termination sequence of a rho-independent terminator? What would happen if a mutation occurred in this region?

What happens during the elongation step of transcription?

What would be the advantage of having multiple sigma factors that each could be produced under different stress conditions in E. coli?

All genes encode proteins.

RNA polymerase has no proofreading activity during transcription.

The single strand of mRNA that is produced during transcription has the same polarity as the ________ strand of DNA.

What kind of experiment or analysis could you conduct in order to delineate the promoter elements of protein-coding genes?

The sigma factor of RNA polymerase is necessary for

A prokaryotic mRNA transcript

Genetics of Bacteria and Bacteriophages

Variations in Chromosome Structure and Number

Advanced Gene Mapping in Eukaryotes

Gene Mapping in Eukaryotes

Quantitative Genetics

Extensions of Mendelian Genetic Principles

Chromosomal Basis of Inheritance

Molecular Evolution

Population Genetics

Non-Mendelian Inheritance

Genetics of Cancer

Regulation of Gene Expression in Eukaryotes

Mendelian Genetics

Regulation of Gene Expression in Bacteria and Bacteriophages

Genomics

Applications of Recombinant DNA Technology

Recombinant DNA Technology

DNA Mutation, DNA Repair, and Transposable Elements

Gene Expression: Translation

Gene Expression: Transcription

Gene Control of Proteins

DNA Replication

DNA: The Genetic Material

Genetics: an Introduction

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