Question 1

Southern blotting is a technique that can be used to identify microbes that cannot be cultured.

Accepted Answer

A) True 
 B)False

Question 2

Which of the following is generally NOT considered an ethical issue regarding recombinant DNA technology?

Accepted Answer

A)  the modification of crop plants 
B)  screening of humans for genes that predispose them to disease 
C)  unforeseen impact on the environment 
D)  the modification of animals to produce pharmaceuticals for humans 
E)  answering basic research questions 
A)  the modification of crop plants 
B)  screening of humans for genes that predispose them to disease 
C)  unforeseen impact on the environment 
D)  the modification of animals to produce pharmaceuticals for humans 
E)  answering basic research questions

Question 3

Compare and contrast the two major categories of restriction enzymes.

Accepted Answer

All restriction enzymes recognize specif

Question 4

Outline a procedure for producing a useful new recombinant product.

Accepted Answer

Many variations are possible.The answer

Question 5

Reverse transcriptase synthesizes a DNA molecule from an RNA template.

Accepted Answer

A) True 
 B)False

Question 6

Which of the following procedures would be used to introduce DNA into a single mouse cell?

Accepted Answer

A)  Southern blotting 
B)  electroporation 
C)  gene gun 
D)  microinjection 
E)  protoplast fusion 
A)  Southern blotting 
B)  electroporation 
C)  gene gun 
D)  microinjection 
E)  protoplast fusion

Question 7

Which of the following is NOT a goal of recombinant DNA technology?

Accepted Answer

A)  combining genetic material from more than one organism to produce new useful organisms 
B)  making it possible to clone humans 
C)  eliminating undesirable traits from livestock or crops 
D)  being able to remove or correct damaging traits in humans 
E)  creating organisms capable of producing useful products 
A)  combining genetic material from more than one organism to produce new useful organisms 
B)  making it possible to clone humans 
C)  eliminating undesirable traits from livestock or crops 
D)  being able to remove or correct damaging traits in humans 
E)  creating organisms capable of producing useful products

Question 8

Small single strand DNA molecules with a fluorescent molecule attached that are used to identify a clone containing a specific gene fragment is called a

Accepted Answer

A)  primer. 
B)  probe. 
C)  plasmid. 
D)  promoter. 
E)  peptide. 
A)  primer. 
B)  probe. 
C)  plasmid. 
D)  promoter. 
E)  peptide.

Question 9

If all the following DNA fragments were analyzed on an electrophoresis gel, which one would migrate farthest from the negative electrode?

Accepted Answer

A)  5000 base pairs 
B)  750 base pairs 
C)  1000 base pairs 
D)  250 base pairs 
E)  2500 base pairs 
A)  5000 base pairs 
B)  750 base pairs 
C)  1000 base pairs 
D)  250 base pairs 
E)  2500 base pairs

Question 10

Synthetic nucleic acids are useful as

Accepted Answer

A)  DNA probes. 
B)  primers for PCR. 
C)  antisense RNAs. 
D)  DNA probes and antisense RNAs. 
E)  DNA probes, primers, and antisense RNAs. 
A)  DNA probes. 
B)  primers for PCR. 
C)  antisense RNAs. 
D)  DNA probes and antisense RNAs. 
E)  DNA probes, primers, and antisense RNAs.

Question 11

Replacing a patient's defective gene with a fragment containing a functional gene is

Accepted Answer

A)  gene therapy. 
B)  genomics. 
C)  gene cloning. 
D)  gene mapping. 
E)  gene selection. 
A)  gene therapy. 
B)  genomics. 
C)  gene cloning. 
D)  gene mapping. 
E)  gene selection.

Question 12

Single-strand DNA molecules are fixed to a glass slide or nylon membrane for the production of

Accepted Answer

A)  microarrays. 
B)  DNA clones. 
C)  real time PCR probes. 
D)  FISH assays. 
E)  templates for Sanger sequencing. 
A)  microarrays. 
B)  DNA clones. 
C)  real time PCR probes. 
D)  FISH assays. 
E)  templates for Sanger sequencing.

Question 13

Double strand DNA fragments are separated by size using

Accepted Answer

A)  restriction analysis. 
B)  FISH. 
C)  PCR. 
D)  northern blotting. 
E)  gel electrophoresis. 
A)  restriction analysis. 
B)  FISH. 
C)  PCR. 
D)  northern blotting. 
E)  gel electrophoresis.

Question 14

Which of the following restriction enzyme sites would produce blunt-ended fragments? (The arrow represents the cutting site of the enzyme.)

Accepted Answer

A)  C↓CGG 
B)  G↓GATCC 
C)  G↓AATTC 
D)  CCC↓GGG 
E)  A↓AGCTT 
A)  C↓CGG 
B)  G↓GATCC 
C)  G↓AATTC 
D)  CCC↓GGG 
E)  A↓AGCTT

Question 15

Restriction enzymes are useful only on synthetic DNAs.

Accepted Answer

A) True 
 B)False

Question 16

Transgenic organisms

Accepted Answer

A)  contain cells from other organisms. 
B)  contain genetically engineered microbes. 
C)  are the same as clones. 
D)  contain genes from other organisms. 
E)  have genomes that have been sequenced completely. 
A)  contain cells from other organisms. 
B)  contain genetically engineered microbes. 
C)  are the same as clones. 
D)  contain genes from other organisms. 
E)  have genomes that have been sequenced completely.

Question 17

Sticky-end fragments generated by EcoRI will hydrogen bond to any other sticky-end sequence.

Accepted Answer

A) True 
 B)False

Question 18

Which of the following devices is used for PCR?

Accepted Answer

A)  an electrophoresis chamber 
B)  a gene gun 
C)  a DNA sequencer 
D)  a thermocycler 
E)  a nucleic acid synthesis machine 
A)  an electrophoresis chamber 
B)  a gene gun 
C)  a DNA sequencer 
D)  a thermocycler 
E)  a nucleic acid synthesis machine

Question 19

Discuss some of the impacts that tools and techniques of recombinant DNA technology have had on medicine.

Accepted Answer

Human gene libraries and DNA sequencing

Question 20

Nucleic acid molecules used to deliver new genes to cells are called (plasmids/vectors/clones).

Accepted Answer

The answer of Nucleic acid molecules used to deliver new...

Exam 8: Recombinant DNA Technology

Southern blotting is a technique that can be used to identify microbes that cannot be cultured.

Which of the following is generally NOT considered an ethical issue regarding recombinant DNA technology?

Compare and contrast the two major categories of restriction enzymes.

Outline a procedure for producing a useful new recombinant product.

Reverse transcriptase synthesizes a DNA molecule from an RNA template.

Which of the following procedures would be used to introduce DNA into a single mouse cell?

Which of the following is NOT a goal of recombinant DNA technology?

Small single strand DNA molecules with a fluorescent molecule attached that are used to identify a clone containing a specific gene fragment is called a

If all the following DNA fragments were analyzed on an electrophoresis gel, which one would migrate farthest from the negative electrode?

Synthetic nucleic acids are useful as

Replacing a patient's defective gene with a fragment containing a functional gene is

Single-strand DNA molecules are fixed to a glass slide or nylon membrane for the production of

Double strand DNA fragments are separated by size using

Which of the following restriction enzyme sites would produce blunt-ended fragments? (The arrow represents the cutting site of the enzyme.)

Restriction enzymes are useful only on synthetic DNAs.

Transgenic organisms

Sticky-end fragments generated by EcoRI will hydrogen bond to any other sticky-end sequence.

Which of the following devices is used for PCR?

Discuss some of the impacts that tools and techniques of recombinant DNA technology have had on medicine.

Nucleic acid molecules used to deliver new genes to cells are called (plasmids/vectors/clones).

A Brief History of Microbiology

The Chemistry of Microbiology

Cell Structure and Function

Microscopy, Staining, and Classification

Microbial Metabolism

Microbial Nutrition and Growth

Microbial Genetics

Controlling Microbial Growth in the Environment

Controlling Microbial Growth in the Body: Antimicrobial Drugs

Characterizing and Classifying Prokaryotes

Characterizing and Classifying Eukaryotes

Characterizing and Classifying Viruses, Viroids, and Prions

Infection, Infectious Diseases, and Epidemiology

Innate Immunity

Adaptive Immunity

Immunization and Immune Testing

Immune Disorders

Microbial Diseases of the Skin and Wounds

Microbial Diseases of the Nervous System and Eyes

Microbial Cardiovascular and Systemic Diseases

Microbial Diseases of the Respiratory System

Microbial Diseases of the Digestive System

Microbial Diseases of the Urinary and Reproductive Systems

Applied and Industrial Microbiology

Microbial Ecology and Microbiomes

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