Question 1

A DNA (library/microarray/screen) may be used to study the complex, changing patterns of mRNA production in an organism.

Accepted Answer

The answer of A DNA (library/microarray/screen) may be used to...

Question 2

A procedure using both reverse transcriptase and real time PCR can be used to

Accepted Answer

A)  determine the number of copies of a DNA sequence in a sample. 
B)  determine the number of copies of an RNA sequence in a sample. 
C)  produce DNA fragments for cDNA cloning. 
D)  both determine the number of copies of an RNA sequence in a sample and produce fragments for cDNA cloning. 
E)  produce RNA fragments for cloning. 
A)  determine the number of copies of a DNA sequence in a sample. 
B)  determine the number of copies of an RNA sequence in a sample. 
C)  produce DNA fragments for cDNA cloning. 
D)  both determine the number of copies of an RNA sequence in a sample and produce fragments for cDNA cloning. 
E)  produce RNA fragments for cloning.

Question 3

Gene therapy for human genetic diseases has not been successful yet.

Accepted Answer

A) True 
 B)False

Question 4

Synthesis of cDNA requires the use of

Accepted Answer

A)  DNA ligase. 
B)  agarose. 
C)  reverse transcriptase. 
D)  restriction enzymes. 
E)  fluorescent synthetic nucleotides. 
A)  DNA ligase. 
B)  agarose. 
C)  reverse transcriptase. 
D)  restriction enzymes. 
E)  fluorescent synthetic nucleotides.

Question 5

Which method of inserting DNA into cells is illustrated in Figure 8.1?

Accepted Answer

A)  protoplast fusion 
B)  injection 
C)  electroporation 
D)  a gene gun 
E)  transduction 
A)  protoplast fusion 
B)  injection 
C)  electroporation 
D)  a gene gun 
E)  transduction

Question 6

Crop plants modified to be resistant to insect damage produce Bt toxin from a (bacterial/fungal/synthetic) gene.

Accepted Answer

The answer of Crop plants modified to be resistant to...

Question 7

Which of the following items is NOT a part of the name of a restriction enzyme?

Accepted Answer

A)  the Gram reaction of the source bacterium 
B)  the specific epithet of the source bacterium 
C)  Roman numerals to indicate its order of discovery 
D)  the strain of the source bacterium 
E)  the genus of the source bacterium 
A)  the Gram reaction of the source bacterium 
B)  the specific epithet of the source bacterium 
C)  Roman numerals to indicate its order of discovery 
D)  the strain of the source bacterium 
E)  the genus of the source bacterium

Question 8

A northern blot differs from a Southern blot in the

Accepted Answer

A)  type of probe used. 
B)  presence or absence of a nitrocellulose membrane. 
C)  size of the genetic sequences involved. 
D)  number of genetic sequences detected. 
E)  type of nucleic acid being isolated. 
A)  type of probe used. 
B)  presence or absence of a nitrocellulose membrane. 
C)  size of the genetic sequences involved. 
D)  number of genetic sequences detected. 
E)  type of nucleic acid being isolated.

Question 9

The microbes residing on human skin are part of the human microbiome.

Accepted Answer

A) True 
 B)False

Question 10

Which of the following is essential in PCR?

Accepted Answer

A)  DNA polymerase 
B)  antisense RNAs 
C)  reverse transcriptase 
D)  DNA primers 
E)  both DNA primers and DNA polymerase 
A)  DNA polymerase 
B)  antisense RNAs 
C)  reverse transcriptase 
D)  DNA primers 
E)  both DNA primers and DNA polymerase

Question 11

In Southern blotting, DNA molecules are immobilized on

Accepted Answer

A)  silicon chips. 
B)  nitrocellulose membranes. 
C)  agarose. 
D)  gold beads. 
E)  paper. 
A)  silicon chips. 
B)  nitrocellulose membranes. 
C)  agarose. 
D)  gold beads. 
E)  paper.

Question 12

Which of the following is a product of recombinant DNA technology?

Accepted Answer

A)  rice that produces vitamin C 
B)  bacteria that produce insulin 
C)  yeast that produces alcohol 
D)  bacteria that produce acetone 
E)  microbes that digest cellulose 
A)  rice that produces vitamin C 
B)  bacteria that produce insulin 
C)  yeast that produces alcohol 
D)  bacteria that produce acetone 
E)  microbes that digest cellulose

Question 13

Restriction (enzymes/proteases/polymerases), first isolated from bacterial cells, cut DNA molecules at specific sites.

Accepted Answer

The answer of Restriction (enzymes/proteases/polymerases), first isolated from bacterial cells,...

Question 14

What is the function of the high temperature step in PCR (polymerase chain reaction)?

Accepted Answer

A)  activation of the DNA polymerase 
B)  allowing primers to bind complementary sequences 
C)  facilitate DNA synthesis 
D)  deactivate DNA polymerase to pause synthesis 
E)  break hydrogen bonds for strand separation 
A)  activation of the DNA polymerase 
B)  allowing primers to bind complementary sequences 
C)  facilitate DNA synthesis 
D)  deactivate DNA polymerase to pause synthesis 
E)  break hydrogen bonds for strand separation

Question 15

Subunit vaccines are safer than traditional vaccines because they

Accepted Answer

A)  are acellular. 
B)  are administered in food. 
C)  do not pose a risk for causing the disease. 
D)  are acellular and do not pose a risk for causing the disease. 
E)  are acellular and can be administered in food. 
A)  are acellular. 
B)  are administered in food. 
C)  do not pose a risk for causing the disease. 
D)  are acellular and do not pose a risk for causing the disease. 
E)  are acellular and can be administered in food.

Exam 8: Recombinant DNA Technology

A DNA (library/microarray/screen) may be used to study the complex, changing patterns of mRNA production in an organism.

A procedure using both reverse transcriptase and real time PCR can be used to

Gene therapy for human genetic diseases has not been successful yet.

Synthesis of cDNA requires the use of

Which method of inserting DNA into cells is illustrated in Figure 8.1?

Crop plants modified to be resistant to insect damage produce Bt toxin from a (bacterial/fungal/synthetic) gene.

Which of the following items is NOT a part of the name of a restriction enzyme?

A northern blot differs from a Southern blot in the

The microbes residing on human skin are part of the human microbiome.

Which of the following is essential in PCR?

In Southern blotting, DNA molecules are immobilized on

Which of the following is a product of recombinant DNA technology?

Restriction (enzymes/proteases/polymerases), first isolated from bacterial cells, cut DNA molecules at specific sites.

What is the function of the high temperature step in PCR (polymerase chain reaction)?

Subunit vaccines are safer than traditional vaccines because they

A Brief History of Microbiology

The Chemistry of Microbiology

Cell Structure and Function

Microscopy, Staining, and Classification

Microbial Metabolism

Microbial Nutrition and Growth

Microbial Genetics

Controlling Microbial Growth in the Environment

Controlling Microbial Growth in the Body: Antimicrobial Drugs

Characterizing and Classifying Prokaryotes

Characterizing and Classifying Eukaryotes

Characterizing and Classifying Viruses, Viroids, and Prions

Infection, Infectious Diseases, and Epidemiology

Innate Immunity

Adaptive Immunity

Immunization and Immune Testing

Immune Disorders

Microbial Diseases of the Skin and Wounds

Microbial Diseases of the Nervous System and Eyes

Microbial Cardiovascular and Systemic Diseases

Microbial Diseases of the Respiratory System

Microbial Diseases of the Digestive System

Microbial Diseases of the Urinary and Reproductive Systems

Applied and Industrial Microbiology

Microbial Ecology and Microbiomes

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