Exam 8: Microbial Genetics and Genetic Engineering

A 59-year-old female has been diagnosed with Escherichia coli infection following the consumption of contaminated meat. The patient is exhibiting a high fever, severe abdominal cramping, and diarrhea. The RN provides supportive care and fluid resuscitation as ordered. The nurse and medical team provide education to the patient and her family regarding the genetics of the E. coli bacterium. -In gram-negative bacteria,like E.coli,the fertility factor is a plasmid possessed by the bacterium that will donate its DNA to a recipient cell.Which of the following statements by the patient demonstrates an understanding of this plasmid?  

Eukaryotic chromosomes differ from bacterial chromosomes because only eukaryotes have ________.

DNA photolyase,in the presence of visible light,can repair DNA damage due to ultraviolet radiation.

Vectors often contain a gene conferring drug resistance to their cloning host,in order to detect cells harboring the plasmid.

Which of the following is not associated with a bacterial ribosome?

Okazaki fragments are attached to the growing end of the lagging strand by ________.

The transfer of DNA fragments from a dead cell to a live,competent recipient cell that results in a changed recipient cell is ________.

The expression of genetic traits is the ________.

Viruses are often used as cloning hosts in recombinant DNA methods.

Which of the following is not true of conjugation?

Split genes ________.

The various techniques by which scientists manipulate DNA in the lab are called ________.

A recombinant organism is one that ________.

Which is incorrect about inducible operons?

Replication of DNA begins at a(n)________ rich area.

Which is incorrect about purines?

Base pairs in DNA are held together by ________ bonds.

During replication,each parent DNA strand serves as a ________ for synthesis of new DNA strands.

When bacteriophages invade bacterial cells,they leave behind DNA that is recognized by the bacteria as foreign and can be eliminated in the event of another attack,reminiscent of adaptive immunity.This natural process can be adapted in the lab so that a combination of guide DNA and nucleases facilitate the cutting of DNA at a precise location in any organism.  Here we are describing ________.

The primers in PCR are ________.