Exam 2: Tools of the Laboratory: Methods for the Culturing and Microscopic Analysis of Microorganisms

The relationship between a micrometer and a millimeter is ________.

Which of the following magnifies the specimen to produce its virtual image?

Which type of medium is able to distinguish different species or types of microorganisms based on an observable change in the colonies or in the medium?

Which of the following is essential for development of discrete,isolated colonies?

The correct microbiological term for the tiny sample of specimen that is put into a nutrient medium in order to produce a culture is the ________.

At the end of the Gram stain,gram-positive bacteria will be seen as red/pink cells.

All of the following are diameters of cells that would be resolved in a microscope with a 0.2 µm limit of resolution except ________.

A pure culture contains ________.

Bacteria that require special growth factors and complex nutrients are termed ________.

Which type of microscope shows cells against a bright background but also differentiates intracellular structures of unstained cells based on their varying densities?

An enveloped virus measures 0.02 micrometers (μm)in diameter. What is the diameter of this virus in nanometers (nm)?

Which type of media would be the best choice when shipping a sample of bacteria to a laboratory to be tested from a satellite office site?

An RN is working at a public health clinic that sees many patients with infectious disease. Ms. Hungh, a Burmese immigrant, presents to the clinic with an interpreter, complaining of fatigue, weight loss, persistent cough, and rust-colored sputum. The interpreter explains that Ms. Hungh has had this cough for many months in her home country and, now that she is in America, is seeking treatment for her condition. -A sputum sample is ordered for microbial analysis in order to rule out the diagnosis of tuberculosis.Suspecting Mycobacterium tuberculosis may be the pathogen,the RN knows the laboratory technicians will perform which stain on the sample?  

One colony typically develops from the growth of several parent bacterial cells.

The diameter of field for a 4x lens is measured at 4.6 mm.  How many bacterial cells,each measuring 4 μm,could be lined up along the diameter?

An RN is working at a public health clinic that sees many patients with infectious disease. Ms. Hungh, a Burmese immigrant, presents to the clinic with an interpreter, complaining of fatigue, weight loss, persistent cough, and rust-colored sputum. The interpreter explains that Ms. Hungh has had this cough for many months in her home country and, now that she is in America, is seeking treatment for her condition. -Ms.Hungh's acid-fast stain results resulted as inconclusive for the presence of acid-fast bacilli.Culturing of the sputum is performed in order to isolate microbial growth for further analysis.Lowenstein-Jensen medium is utilized to select for the growth of Mycobacterium species if present in the sample,while suppressing unwanted background organisms.What is the proper term for this type of medium?  

The three physical forms of laboratory media are ________.

A nutrient medium that has all of its chemical components identified,and their precise concentrations known and reproducible,would be termed ________.

A rod-shaped bacterium is measured as 0.3 micrometers (μm)in length using an ocular micrometer. Your instructor wants you to report the length in millimeters (mm)to test your understanding of metric conversions. What is the length of the organism in millimeters?

A microbiologist inoculates Staphylococcus aureus into a culture medium.Following incubation,both Staphylococcus aureus and Staphylococcus epidermidis are determined to be growing in this culture.What is the most likely explanation?