Exam 20: DNA Tools and Biotechnology

One successful form of gene therapy has involved delivery of an allele for the enzyme adenosine deaminase (ADA)to bone marrow cells of a child with severe combined immunodeficiency (SCID),and delivery of these engineered cells back to the bone marrow of the affected child.What is one major reason for the success of this procedure as opposed to many other efforts at gene therapy?

Which of the following is most closely identical to the formation of twins?

Modern forensic investigation uses ________ to identify criminals.

To introduce a particular piece of DNA into an animal cell,such as that of a mouse,you would find more probable success with which of the following methods?

What is the most logical sequence of steps for splicing foreign DNA into a plasmid and inserting the plasmid into a bacterium? I.Transform bacteria with a recombinant DNA molecule. II.Cut the plasmid DNA using restriction enzymes. III.Extract plasmid DNA from bacterial cells. IV.Hydrogen-bond the plasmid DNA to nonplasmid DNA fragments. V.Use ligase to seal plasmid DNA to nonplasmid DNA.

A paleontologist has recovered a bit of tissue from the 400-year-old preserved skin of an extinct dodo (a bird).To compare a specific region of the DNA from the sample with DNA from living birds,which of the following would be most useful for increasing the amount of dodo DNA available for testing?

An unspecialized cell that can reproduce and differentiate is a(n)________ cell.

Which of the following techniques used to analyze gene function depends on the specificity of DNA base complementarity?

Which of the following is true of embryonic stem cells but not of adult stem cells?

Use the following information to answer the next few questions. A group of six students has taken samples of their own cheek cells,purified the DNA,and used a restriction enzyme known to cut at zero,one,or two sites in a particular gene of interest. -Their next two steps,in order,should be

Why is it so important to be able to amplify DNA fragments when studying genes?

Which of the following best describes the complete sequence of steps occurring during every cycle of PCR? 1)The primers hybridize to the target DNA. 2)The mixture is heated to a high temperature to denature the double-stranded target DNA. 3)Fresh DNA polymerase is added. 4)DNA polymerase extends the primers to make a copy of the target DNA.

Which of the following statements is (are)correct about restriction enzymes? I.They are used by bacteria to cut up foreign DNA. II.They cut DNA unevenly to produce "sticky ends." III.They are non-specific and cut anywhere along a sequence. IV.They are involved with heterochromatin production.

Reproductive cloning of human embryos is generally considered unethical.However,on the subject of therapeutic cloning there is a wider divergence of opinion.Which of the following is a likely explanation?

Which of the following would not be true of cDNA produced using human brain tissue as the starting material?

Pax-6 is a gene that is involved in eye formation in many invertebrates,such as Drosophila.Pax-6 is found as well in vertebrates.A Pax-6 gene from a mouse can be expressed in a fly and the protein (PAX-6)leads to a compound fly eye.This information suggests which of the following?

In an experiment,the bacteriophage Lambda genome was cut into fragments using the restriction enzyme Hind III.Hind III cuts the Lambda producing eight different size fragments when separated by gel electrophoresis.Select an observation that best describes a correct aspect of the two processes of restriction digest and gel electrophoresis:

Which of the following is one of the technical reasons why gene therapy is problematic?