Exam 1: An Overview of Cells and Cell Research

With appropriate stimulation, mammalian stem cells in culture can give rise to individual mammalian cell types and hence may provide a source of patient-specific replacement cells.

What technique would be best for separating rough endoplasmic reticulum derived membrane vesicles from smooth endoplasmic reticulum membrane vesicles?

RNA is believed to have been the original genetic system because it can

Which fish is proving to be a useful model organism for the study of vertebrate development?

_______ is a light microscope technique for studying the interaction of proteins.

Immortal cell lines are cells that can continue to proliferate in culture for an indefinite number of generations.

Studies on Arabidopsis thaliana have indicated that the mechanisms that control development in plants are completely different from those that control development in animals.

The frog Xenopus laevis is useful for studying early development because its eggs are small and clear.

Imagine you are studying an inducible transcription factor called X. You make a cellular lysate and carry out a series of centrifugation steps at increasing speeds to localize X. You find that prior to induction, X is in the supernatant after a fourth, very high speed ultracentrifugation step; however, after induction it is found in the pellet after only one, relatively low speed ultracentrifugation step. What might this tell you about how X is regulated? What other techniques might you carry out to confirm your results?

Similarities in basic metabolic mechanisms indicate that all present-day cells on Earth descended from a single primordial ancestor.

What technique would be best for observing the detailed structure of the surface of single-celled eukaryotes like Paramecium?

Scanning electron microscopy is usually used for a three-dimensional view of the surface of cells.

Which of the following is a high-resolution light microscope technique for detecting interactions between proteins?

Cultures grown from cells of a dissociated tissue are called

How many genes does an E. coli have?

In transmission electron microscopy, objects are imaged by their differences in _______ density.

It is generally believed that Earth's original cell enclosed a self-replicating _______ molecule.

Xenopus laevis, an amphibian vertebrate, is useful for developmental studies because when its eggs are fertilized, the embryos develop outside its body.

Approximately how many doublings can normal human fibroblasts undergo in culture?

An E. coli cell under well-defined laboratory conditions divides about every