Question 1

Why are E. coli DNA polymerases not used in the polymerase chain reaction (PCR)?

Accepted Answer

E. coli DNA polymerases cannot be used in PCR because they are not stable at the temperatures required to denature the DNA strands in the first step of each PCR cycle-the enzyme would rapidly be inactivated, and polymerization would cease. Instead, polymerases from bacteria that have evolved to live at high temperatures (such as Thermus aquaticus) are used, since they are stable at high temperatures.

Question 2

Mutations may be introduced into selected genes of living cells by

Accepted Answer

A)  homologous recombination with an altered cloned gene. 
B)  hybridization with antisense nucleic acid sequences. 
C)  the polymerase chain reaction. 
D)  ultraviolet laser irradiation. 
A)  homologous recombination with an altered cloned gene. 
B)  hybridization with antisense nucleic acid sequences. 
C)  the polymerase chain reaction. 
D)  ultraviolet laser irradiation. A

Question 3

The three codons that do not specify amino acids serve as _______.

Accepted Answer

stop codons

Question 4

The CRISPR/Cas system can knock out the expression of a gene in vivo and/or introduce a new mutation into a gene.

Accepted Answer

A) True 
 B)False

Question 5

The enzyme discovered by Temin, Mizutani, and Baltimore, which synthesizes DNA from an RNA template, is called _______

Accepted Answer

reverse transcriptas

Question 6

To identify the protein against which a given sample has an antibody, the best technique to use would be _______ blotting.

Accepted Answer

A)  Southern 
B)  Northern 
C)  Western 
D)  Eastern 
A)  Southern 
B)  Northern 
C)  Western 
D)  Eastern

Question 7

DNA replicates

Accepted Answer

A)  conservatively. 
B)  semiconservatively. 
C)  liberally. 
D)  by hybridization. 
A)  conservatively. 
B)  semiconservatively. 
C)  liberally. 
D)  by hybridization.

Question 8

Animal cells and most cells of higher plants are

Accepted Answer

A)  haploid. 
B)  diploid. 
C)  euploid. 
D)  tetraploid. 
A)  haploid. 
B)  diploid. 
C)  euploid. 
D)  tetraploid.

Question 9

Suppose you have a cell line that expresses a protein whose structure you know but not the function. You use RNA interference to block expression of the protein. How could you confirm that the interference was effective?

Accepted Answer

Since you know the structure of the prot

Question 10

In the base pairing within a DNA double helix,

Accepted Answer

A)  purines pair with purines. 
B)  A pairs with U and G pairs with C. 
C)  A pairs with G and C pairs with T. 
D)  A pairs with T and G pairs with C. 
A)  purines pair with purines. 
B)  A pairs with U and G pairs with C. 
C)  A pairs with G and C pairs with T. 
D)  A pairs with T and G pairs with C.

Question 11

A poly-U RNA template codes only for the amino acid _______.

Accepted Answer

The answer of A poly-U RNA template codes only for...

Question 12

A cDNA is especially useful for bacterial expression of a eukaryotic protein.

Accepted Answer

A) True 
 B)False

Question 13

Suppose you are studying a mammalian transcription factor that you would like to express in bacteria so that you can purify large quantities of it to use in biochemical studies. You introduce the cDNA encoding the transcription factor into an expression plasmid and transform E. coli with the new recombinant vector. You find no expression. What might be occurring?

Accepted Answer

Lack of expression could result from a n

Question 14

EcoRI restriction mapping on a sample of a large piece of DNA resulted in six distinct bands following agarose gel electrophoresis. Similar mapping on an identical sample that was subjected to high intensity ultraviolet light resulted in only four distinct bands. What might best explain the reduction in the number of detectable bands in the UV light-treated sample?

Accepted Answer

EcoRI specifically cleaves DNA at GAATTC

Question 15

RNA viruses that replicate via a DNA intermediate are called _______.

Accepted Answer

The answer of RNA viruses that replicate via a DNA...

Question 16

A codon is a

Accepted Answer

A)  region of DNA coding for one protein. 
B)  sequence of three nucleotides on a tRNA that binds to an mRNA. 
C)  sequence of three nucleotides on an mRNA that binds to specific tRNAs. 
D)  sequence of three nucleotides on the coding strand of DNA. 
A)  region of DNA coding for one protein. 
B)  sequence of three nucleotides on a tRNA that binds to an mRNA. 
C)  sequence of three nucleotides on an mRNA that binds to specific tRNAs. 
D)  sequence of three nucleotides on the coding strand of DNA.

Question 17

The genetic code is said to be degenerate. What does "degenerate" mean in this context?

Accepted Answer

The genetic code is based on multiples o

Question 18

Vectors for expressing a eukaryotic protein in a bacterium must contain

Accepted Answer

A)  a eukaryotic promoter. 
B)  antibody recognition sequences. 
C)  an origin of replication. 
D)  EcoRI restriction enzyme recognition sites. 
A)  a eukaryotic promoter. 
B)  antibody recognition sequences. 
C)  an origin of replication. 
D)  EcoRI restriction enzyme recognition sites.

Question 19

DNA contains the base uracil, whereas RNA contains the base thymine.

Accepted Answer

A) True 
 B)False

Question 20

Meiosis results in a change of nuclear DNA from haploid to diploid.

Accepted Answer

A) True 
 B)False

Why are E. coli DNA polymerases not used in the polymerase chain reaction (PCR)?

Mutations may be introduced into selected genes of living cells by

The three codons that do not specify amino acids serve as _______.

The CRISPR/Cas system can knock out the expression of a gene in vivo and/or introduce a new mutation into a gene.

The enzyme discovered by Temin, Mizutani, and Baltimore, which synthesizes DNA from an RNA template, is called _______

To identify the protein against which a given sample has an antibody, the best technique to use would be _______ blotting.

DNA replicates

Animal cells and most cells of higher plants are

Suppose you have a cell line that expresses a protein whose structure you know but not the function. You use RNA interference to block expression of the protein. How could you confirm that the interference was effective?

In the base pairing within a DNA double helix,

A poly-U RNA template codes only for the amino acid _______.

A cDNA is especially useful for bacterial expression of a eukaryotic protein.

RNA viruses that replicate via a DNA intermediate are called _______.

A codon is a

The genetic code is said to be degenerate. What does "degenerate" mean in this context?

Vectors for expressing a eukaryotic protein in a bacterium must contain

DNA contains the base uracil, whereas RNA contains the base thymine.

Meiosis results in a change of nuclear DNA from haploid to diploid.

An Overview of Cells and Cell Research

Molecules and Membranes

Bioenergetics and Metabolism

Genomics, Proteomics, and Systems Biology

Genes and Genomes

Replication, Maintenance, and Rearrangements of Genomic Dna

RNA Synthesis and Processing

Transcriptional Regulation and Epigenetics

Protein Synthesis, Processing, and Regulation

The Nucleus

Protein Sorting and Transport

Mitochondria, Chloroplasts, and Peroxisomes

The Cytoskeleton and Cell Movement

The Plasma Membrane

Cell Walls, the Extracellular Matrix, and Cell Interactions

Cell Signaling

The Cell Cycle

Cell Death and Cell Renewal

Cancer

Filters

Exam 4: Fundamentals of Molecular Biology

Why are E. coli DNA polymerases not used in the polymerase chain reaction (PCR)?

Mutations may be introduced into selected genes of living cells by

The three codons that do not specify amino acids serve as _______.

The CRISPR/Cas system can knock out the expression of a gene in vivo and/or introduce a new mutation into a gene.

The enzyme discovered by Temin, Mizutani, and Baltimore, which synthesizes DNA from an RNA template, is called _______

To identify the protein against which a given sample has an antibody, the best technique to use would be _______ blotting.

DNA replicates

Animal cells and most cells of higher plants are

Suppose you have a cell line that expresses a protein whose structure you know but not the function. You use RNA interference to block expression of the protein. How could you confirm that the interference was effective?

In the base pairing within a DNA double helix,

A poly-U RNA template codes only for the amino acid _______.

A cDNA is especially useful for bacterial expression of a eukaryotic protein.

RNA viruses that replicate via a DNA intermediate are called _______.

A codon is a

The genetic code is said to be degenerate. What does "degenerate" mean in this context?

Vectors for expressing a eukaryotic protein in a bacterium must contain

DNA contains the base uracil, whereas RNA contains the base thymine.

Meiosis results in a change of nuclear DNA from haploid to diploid.

An Overview of Cells and Cell Research

Molecules and Membranes

Bioenergetics and Metabolism

Genomics, Proteomics, and Systems Biology

Genes and Genomes

Replication, Maintenance, and Rearrangements of Genomic Dna

RNA Synthesis and Processing

Transcriptional Regulation and Epigenetics

Protein Synthesis, Processing, and Regulation

The Nucleus

Protein Sorting and Transport

Mitochondria, Chloroplasts, and Peroxisomes

The Cytoskeleton and Cell Movement

The Plasma Membrane

Cell Walls, the Extracellular Matrix, and Cell Interactions

Cell Signaling

The Cell Cycle

Cell Death and Cell Renewal

Cancer

Filters