Exam 6: Genetic Analysis and Mapping in Bacteria and Bacteriophages

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What are the roles of the recBCD genes?

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They function in bacterial recombination.

Compare and contrast bacteriophage lysis and lysogeny.

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Both lysis and lysogeny involve bacterial/phage interactions related to the production of phage progeny.Lysis occurs when progeny phage burst from the bacterial cell; lysogeny involves the incorporation of the phage chromosome into the bacterial chromosome.

Name the general category into which double-stranded circular extrachromosomal DNA elements such as F factors,ColE1,and R would fall.

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E

What is meant by the term cotransformation?

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What is a significant difference between a lytic and a lysogenic cycle?

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Distinguish between F⁺ and F⁻ bacteria.

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Describe how different strains of E.coli can reveal different linkage arrangements of genes in Hfr crosses.

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R plasmids often contain genes for antibody production.

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The clearing made by bacteriophages in a "lawn" of bacteria on an agar plate is called a ________.

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A form of bacterial recombination that involves a viral intermediate is called ________.

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What experimental observation demonstrated that genes are involved in the integration of foreign DNA into the bacterial chromosome?

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Lysogeny is a process that occurs during transformation and conjugation.

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To produce recombinants in bacteria,one crossover is better than two.

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Explain the composition and use of minimal medium in the study of bacterial genetics.

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Temperate phages are those that can enter either the ________ or ________ cycle.

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The "interrupted mating technique" provides a genetic map in Drosophila.

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Lysogeny is an important phenomenon in bacteria and phages.Briefly describe lysogeny (using labeled diagrams if helpful).

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Assume that one counted 67 plaques on a bacterial plate where 0.1 ml of a 10⁻⁵ dilution of phage was added to bacterial culture.What is the initial concentration of the undiluted phage?

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Name three forms of recombination in bacteria.

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Name the term used to describe the phenomenon in which a bacteriophage genome incorporates its genome into the chromosome of the host.

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