Exam 6: Genetic Analysis and Mapping in Bacteria and Bacteriophages

In general,what two methods are used to grow bacteria in the laboratory?

How does an auxotroph differ from a prototroph?

Bacteriophages engage in two interactive cycles with bacteria.What are these cycles?

Bacteriophages that cannot undergo lysogeny but can infect bacteria are called ________.

What are prototrophs?

What is a form of recombination in bacteria that involves the F plasmid?

When a bacteriophage genome incorporates itself into the chromosome of the host,that phage genome is referred to as a(n)________.

Viral mutations and variants are often categorized by changes in host range and/or plaque morphology.

Name two forms of recombination in bacteria.

Assume that the gene trpA in an auxotrophic strain of E.coli is located at 27 minutes,whereas the gene pyrE is located at 81 minutes. a) How are minutes arrived at in this context? b) Present an experimental scheme that would allow you to convert one of the auxotrophic strains to a prototrophic strain.

A plaque is a substance that causes mutation in bacteria.

Explain what is meant by the term heteroduplex in the context of bacterial transformation.

Jacob,Wollman,and others developed a linkage map of E.coli that is based on time.What form of recombination is involved in generating a linkage map based on time?

Cotransduction of genes is an indication that the genes are linked.

An Hfr cell can initiate chromosome transfer from one E.coli to another.

Lysogeny is most likely associated with transduction.

What is the consequence of a mutation in the recA gene in bacteria?

In what way was the interrupted mating technique used to generate a genetic map in E.coli based on time?

Present the general structural features of a plasmid and give an example.

Name the typical phases of the bacterial growth cycle in liquid culture medium.