Question 1

In the classic experiment conducted by Hershey and Chase,why was the pellet radioactive in the centrifuge tube that contained bacteria with viruses?

Accepted Answer

A)  The bacteria were in the pellet, and they had incorporated radioactive proteins into their cell membranes. 
B)  The radioactive viruses (coats plus DN
A)  were in the pellet. 
C)  The bacteria were in the pellet, and many contained the radioactive viral DNA. 
D)  The radioactive protein coats of the viruses were in the pellet. 
E)  The radioactive viruses were in the pellet, and the bacteria were in the supernatant. 
A)  The bacteria were in the pellet, and they had incorporated radioactive proteins into their cell membranes. 
B)  The radioactive viruses (coats plus DN
A)  were in the pellet. 
C)  The bacteria were in the pellet, and many contained the radioactive viral DNA. 
D)  The radioactive protein coats of the viruses were in the pellet. 
E)  The radioactive viruses were in the pellet, and the bacteria were in the supernatant.

Question 2

What does it mean to say that double-stranded nucleic acids are antiparallel?

Accepted Answer

The C-5′ to C-3′ ori

Question 3

Consider the structure of double-stranded DNA.When DNA is placed into distilled water,it denatures; however,by adding NaCl,the DNA renatures.Why?

Accepted Answer

The negatively charged phosphates repel

Question 4

Reverse transcriptase is an enzyme found in association with retroviral activity.It has the property of ________.

Accepted Answer

A)  synthesis of DNA from an RNA template 
B)  synthesis of RNA from a DNA template 
C)  requiring no template 
D)  translation 
E)  most lysozymes 
A)  synthesis of DNA from an RNA template 
B)  synthesis of RNA from a DNA template 
C)  requiring no template 
D)  translation 
E)  most lysozymes

Question 5

Present an overview of two classical experiments that demonstrated that DNA is the genetic material.Can RNA be the genetic material? Explain.

Accepted Answer

(1)Transformation in bacteria (Griffith

Question 6

G and C are present in both DNA and RNA.

Accepted Answer

A) True 
 B)False

Question 7

Avery et al.(1944)determined that DNA is the genetic material in T2 bacteriophage.

Accepted Answer

A) True 
 B)False

Question 8

List two major differences between RNA and DNA at the level of the nucleotide.

Accepted Answer

ribose in RNA,deoxyr

Question 9

In an analysis of the nucleotide composition of double-stranded DNA to see which bases are equivalent in concentration,which of the following would be true?

Accepted Answer

A)  A = C 
B)  A = G and C = T 
C)  A + C = G + T 
D)  A + T = G + C 
E)  A = G and C = T and A + C = G + T are both true. 
A)  A = C 
B)  A = G and C = T 
C)  A + C = G + T 
D)  A + T = G + C 
E)  A = G and C = T and A + C = G + T are both true.

Question 10

When considering the structure of DNA,we would say that complementary strands are antiparallel.

Accepted Answer

A) True 
 B)False

Question 11

What type of chemical bond holds the two strands together in double-stranded DNA?

Accepted Answer

The answer of What type of chemical bond holds the...

Question 12

The transforming principle discovered by Griffith is RNA.

Accepted Answer

A) True 
 B)False

Question 13

Is the accompanying figure DNA or RNA? Is the circle closer to the 5′ or 3′ end?

Accepted Answer

The answer of Is the accompanying figure DNA or RNA?...

Question 14

Name the pyrimidines and the purines in DNA.

Accepted Answer

pyrimidines: cytosin

Question 15

What is the name of the precursor molecule used in nucleic acid synthesis?

Accepted Answer

triphospha

Question 16

During the polymerization of nucleic acids,covalent bonds are formed between neighboring nucleotides.Which carbons are involved in such bonds?

Accepted Answer

The answer of During the polymerization of nucleic acids,covalent bonds...

Question 17

Explain how and why the following circumstances influence characteristics of temperature-induced DNA melting.
a) Percentage of GC base pairs
b) Urea (forms hydrogen bonds with bases)
c) Sodium chloride (neutralizes negatively charged phosphates)

Accepted Answer

GC pairs are composed of three hydrogen

Question 18

Which of the following clusters of terms accurately describes DNA as it is generally viewed to exist in prokaryotes and eukaryotes?

Accepted Answer

A)  double-stranded, parallel, (A + T)/C + G) = variable, (A + G)/(C + T) = 1.0 
B)  double-stranded, antiparallel, (A + T)/C + G) = variable, (A + G)/(C+ T) = 1.0 
C)  single-stranded, antiparallel, (A + T)/C + G) = 1.0, (A + G)/(C + T) = 1.0 
D)  double-stranded, parallel, (A + T)/C + G) = 1.0, (A + G)/(C + T) = 1.0 
E)  double-stranded, antiparallel, (A + T)/C + G) = variable, (A + G)/(C + T) = variable 
A)  double-stranded, parallel, (A + T)/C + G) = variable, (A + G)/(C + T) = 1.0 
B)  double-stranded, antiparallel, (A + T)/C + G) = variable, (A + G)/(C+ T) = 1.0 
C)  single-stranded, antiparallel, (A + T)/C + G) = 1.0, (A + G)/(C + T) = 1.0 
D)  double-stranded, parallel, (A + T)/C + G) = 1.0, (A + G)/(C + T) = 1.0 
E)  double-stranded, antiparallel, (A + T)/C + G) = variable, (A + G)/(C + T) = variable

Question 19

Is the accompanying figure DNA or RNA? Is the circle closer to the 5′ or 3′ end?

Accepted Answer

The answer of Is the accompanying figure DNA or RNA?...

Question 20

What is the difference between a polynucleotide and an oligonucleotide?

Accepted Answer

Polynucleotides are polymers l

Exam 10: DNA Structure and Analysis

In the classic experiment conducted by Hershey and Chase,why was the pellet radioactive in the centrifuge tube that contained bacteria with viruses?

What does it mean to say that double-stranded nucleic acids are antiparallel?

Consider the structure of double-stranded DNA.When DNA is placed into distilled water,it denatures; however,by adding NaCl,the DNA renatures.Why?

Reverse transcriptase is an enzyme found in association with retroviral activity.It has the property of ________.

Present an overview of two classical experiments that demonstrated that DNA is the genetic material.Can RNA be the genetic material? Explain.

G and C are present in both DNA and RNA.

Avery et al.(1944)determined that DNA is the genetic material in T2 bacteriophage.

List two major differences between RNA and DNA at the level of the nucleotide.

In an analysis of the nucleotide composition of double-stranded DNA to see which bases are equivalent in concentration,which of the following would be true?

When considering the structure of DNA,we would say that complementary strands are antiparallel.

What type of chemical bond holds the two strands together in double-stranded DNA?

The transforming principle discovered by Griffith is RNA.

Is the accompanying figure DNA or RNA? Is the circle closer to the 5′ or 3′ end?

Name the pyrimidines and the purines in DNA.

What is the name of the precursor molecule used in nucleic acid synthesis?

During the polymerization of nucleic acids,covalent bonds are formed between neighboring nucleotides.Which carbons are involved in such bonds?

Explain how and why the following circumstances influence characteristics of temperature-induced DNA melting. a) Percentage of GC base pairs b) Urea (forms hydrogen bonds with bases) c) Sodium chloride (neutralizes negatively charged phosphates)

Which of the following clusters of terms accurately describes DNA as it is generally viewed to exist in prokaryotes and eukaryotes?

Is the accompanying figure DNA or RNA? Is the circle closer to the 5′ or 3′ end?

What is the difference between a polynucleotide and an oligonucleotide?

Introduction to Genetics

Mitosis and Meiosis

Mendelian Genetics

Extensions of Mendelian Genetics

Chromosome Mapping in Eukaryotes

Genetic Analysis and Mapping in Bacteria and Bacteriophages

Sex Determination and Sex Chromosomes

Chromosome Mutations: Variation in Number and Arrangement

Extranuclear Inheritance

DNA Replication and Recombination

DNA Organization in Chromosomes

The Genetic Code and Transcription

Translation and Proteins

Gene Mutation,dna Repair,and Transposition

Regulation of Gene Expression in Prokaryotes

Regulation of Gene Expression in Eukaryotes

Developmental Genetics

Cancer and Regulation of the Cell Cycle

Recombinant DNA Technology

Genomics, bioinformatics, and Proteomics

Applications and Ethics of Genetic Engineering and Biotechnology

Quantitative Genetics and Multifactorial Traits

Neurogenetics

Population and Evolutionary Genetics

Filters