Question 1

Which of the following types of cloning vector can carry the largest amount of foreign DNA?

Accepted Answer

A) bacterial artificial chromosome 
B) bacteriophage 
C) cosmid 
D) plasmid 
A) bacterial artificial chromosome 
B) bacteriophage 
C) cosmid 
D) plasmid

Question 2

If all of the PCR products of a DNA sequence are 4000 bp,then the DNA sequence that was amplified must have been 8000 bp.

Accepted Answer

A) True 
 B)False

Question 3

Plasmid DNA having one EcoRI recognition sequence is treated with EcoRI restriction endonuclease.Following gel electrophoresis,how many bands should be visible on the gel?

Accepted Answer

A) One 
B) Two 
C) Four 
D) Zero 
A) One 
B) Two 
C) Four 
D) Zero

Question 4

___________ is a bacterial plasmid vector.

Accepted Answer

A) Lambda 
B) T4 DNA ligase 
C) pUC19 
D) SV40 
A) Lambda 
B) T4 DNA ligase 
C) pUC19 
D) SV40

Question 5

Cosmids are plasmids that can be packaged into capsids of the bacteriophage lambda; therefore,they can be transmitted like phages,but they can exist and replicate in a cell like plasmids.

Accepted Answer

A) True 
 B)False

Question 6

Which of the following bacterial hosts would should be used to avoid degradation of DNA that is introduced via a cloning vector?

Accepted Answer

A) Escherichia coli that lack endonucleases 
B) RecA-expressing Escherchia coli 
C) Escherchia coli RecA mutants 
D) Saccharomyces cerevisiae wild type cells 
A) Escherichia coli that lack endonucleases 
B) RecA-expressing Escherchia coli 
C) Escherchia coli RecA mutants 
D) Saccharomyces cerevisiae wild type cells

Question 7

A (n)__________ vector contains promoters that result in high-level transcription of the gene cloned within a multicloning site.

Accepted Answer

A) shuttle 
B) chimeric 
C) expression 
D) phage 
A) shuttle 
B) chimeric 
C) expression 
D) phage

Question 8

A molecular biologist is interested in purify a recombinant protein by His-tagging,but the protein and vector both lack histidine residues.In this case,the molecular biologist could use which of the following technique(s)to acheive purification?

Accepted Answer

A) Add a 6xHis-tag to the C-terminus of the protein. 
B) Add a series of histidine residues to the N-terminus of the protein. 
C) Amplify the histidine-encoding sequence by PCR and add it to the gene of interest. 
D) All of the choices are correct. 
A) Add a 6xHis-tag to the C-terminus of the protein. 
B) Add a series of histidine residues to the N-terminus of the protein. 
C) Amplify the histidine-encoding sequence by PCR and add it to the gene of interest. 
D) All of the choices are correct.

Question 9

As a newly hired molecular biologist for a company that produces genetically modified seeds,your first project is to ensure that a GM cotton plant carrying a bacterial insecticide gene is expressed in tissues of seedlings as well as mature plants.To better understand regulation of the gene,you decide to use GFP fusion to detect activity of the promoter and also use GFP fusion to determine the location of the protein in plants tissues at various stages of growth.

Accepted Answer

A) transcriptional; translational 
B) translational; transcriptional 
C) transcriptional; transcriptional 
D) translational; translational 
A) transcriptional; translational 
B) translational; transcriptional 
C) transcriptional; transcriptional 
D) translational; translational

Question 10

Cosmids are so named because they can be used to express foreign genes in a variety of different hosts.

Accepted Answer

A) True 
 B)False

Question 11

Which cloning vector should be used to express a 500-kb DNA fragment in Saccharomyces cerevisiae?

Accepted Answer

A) YAC 
B) BAC 
C) pUC19 
D) $\lambda$1059 
A) YAC 
B) BAC 
C) pUC19 
D) $\lambda$1059

Question 12

Which is most analogous to the role of GFP in recombinant DNA technology?

Accepted Answer

A) Using dyes to stain and detect bacteria under light microscopy from the sputum of a patient diagnosed with tuberculosis. 
B) Comparing the RFLP patterns of DNA from various suspects to DNA isolated from the scene of a crime using gel electrophoresis. 
C) Creating a metagenomic library representing archaea bacteria that thrive in the hot springs of Yellowstone National Park. 
D) Monitoring the spread of multi-drug resistant pathogens on a hospital floor by comparing antibiotic susceptibility patterns. 
A) Using dyes to stain and detect bacteria under light microscopy from the sputum of a patient diagnosed with tuberculosis. 
B) Comparing the RFLP patterns of DNA from various suspects to DNA isolated from the scene of a crime using gel electrophoresis. 
C) Creating a metagenomic library representing archaea bacteria that thrive in the hot springs of Yellowstone National Park. 
D) Monitoring the spread of multi-drug resistant pathogens on a hospital floor by comparing antibiotic susceptibility patterns.

Question 13

Genetic engineering methods have been used to produce vaccines.

Accepted Answer

A) True 
 B)False

Question 14

During the investigation of a convenience store robbery,witnesses report that the perpetrator exited through a main entry door without wearing gloves.The police are holding a prime suspect in custody while samples from the door are collected and analyzed for a DNA match.Since the door was handled by many people before the robbery,how will the forensics department distinguish the suspect's DNA from the DNA of others using PCR?

Accepted Answer

A) Sequence-specific primers to the suspect's DNA will be used for PCR amplification. 
B) Sequence-specific primers to the suspect's DNA will be added following PCR amplification. 
C) PCR will be used to make sequence-specific primers that are complementary to the suspect's DNA. 
D) Since multiple people touched the doorknob without gloves,it is impossible to use PCR to distinguish the suspect's DNA. 
A) Sequence-specific primers to the suspect's DNA will be used for PCR amplification. 
B) Sequence-specific primers to the suspect's DNA will be added following PCR amplification. 
C) PCR will be used to make sequence-specific primers that are complementary to the suspect's DNA. 
D) Since multiple people touched the doorknob without gloves,it is impossible to use PCR to distinguish the suspect's DNA.

Question 15

The Southern blotting technique for transferring DNA from an agarose gel to a piece of nitrocellulose membrane is named for

Accepted Answer

A) True 
 B)False

Question 16

Which is a true statement regarding the size of PCR products?

Accepted Answer

A) Since the number of DNA products ending exactly between both primers increases with each cycle,when PCR is completed,the majority of products are of similar size. 
B) Since the number of DNA products extends beyond each primer with each cycle,when PCR is completed,the majority of products are of various sizes. 
C) Since the length of primers increases with each cycle,when PCR is completed,the majority of products are of various sizes. 
D) None of the choices are correct. 
A) Since the number of DNA products ending exactly between both primers increases with each cycle,when PCR is completed,the majority of products are of similar size. 
B) Since the number of DNA products extends beyond each primer with each cycle,when PCR is completed,the majority of products are of various sizes. 
C) Since the length of primers increases with each cycle,when PCR is completed,the majority of products are of various sizes. 
D) None of the choices are correct.

Question 17

Recombinant DNA technology does not rely on which of the following enzymes?

Accepted Answer

A) restriction endonucleases 
B) RNA methylase 
C) DNA ligase 
D) reverse transcriptase 
A) restriction endonucleases 
B) RNA methylase 
C) DNA ligase 
D) reverse transcriptase

Question 18

Antibiotics incorporated into the culture medium can

Accepted Answer

A) select against organisms that have not incorporated the plasmid. 
B) select against organisms that have incorporated a plasmid not containing the desired gene. 
C) enhance production of recombinant proteins. 
D) select against organisms that have not incorporated the plasmid and select against organisms that have incorporated a plasmid not containing the desired gene 
A) select against organisms that have not incorporated the plasmid. 
B) select against organisms that have incorporated a plasmid not containing the desired gene. 
C) enhance production of recombinant proteins. 
D) select against organisms that have not incorporated the plasmid and select against organisms that have incorporated a plasmid not containing the desired gene

Question 19

One of the major advantages to using plasmids as cloning vectors is that very high copy numbers can be achieved with many types of plasmid vectors.

Accepted Answer

A) True 
 B)False

Question 20

The PCR method was developed by

Accepted Answer

A) Boyer. 
B) Mullis. 
C) Cohen. 
D) Sanger. 
A) Boyer. 
B) Mullis. 
C) Cohen. 
D) Sanger.

Exam 17: Recombinant DNA Technology

Which of the following types of cloning vector can carry the largest amount of foreign DNA?

If all of the PCR products of a DNA sequence are 4000 bp,then the DNA sequence that was amplified must have been 8000 bp.

Plasmid DNA having one EcoRI recognition sequence is treated with EcoRI restriction endonuclease.Following gel electrophoresis,how many bands should be visible on the gel?

___________ is a bacterial plasmid vector.

Cosmids are plasmids that can be packaged into capsids of the bacteriophage lambda; therefore,they can be transmitted like phages,but they can exist and replicate in a cell like plasmids.

Which of the following bacterial hosts would should be used to avoid degradation of DNA that is introduced via a cloning vector?

A (n)__________ vector contains promoters that result in high-level transcription of the gene cloned within a multicloning site.

A molecular biologist is interested in purify a recombinant protein by His-tagging,but the protein and vector both lack histidine residues.In this case,the molecular biologist could use which of the following technique(s)to acheive purification?

Cosmids are so named because they can be used to express foreign genes in a variety of different hosts.

Which cloning vector should be used to express a 500-kb DNA fragment in Saccharomyces cerevisiae?

Which is most analogous to the role of GFP in recombinant DNA technology?

Genetic engineering methods have been used to produce vaccines.

The Southern blotting technique for transferring DNA from an agarose gel to a piece of nitrocellulose membrane is named for

Which is a true statement regarding the size of PCR products?

Recombinant DNA technology does not rely on which of the following enzymes?

Antibiotics incorporated into the culture medium can

One of the major advantages to using plasmids as cloning vectors is that very high copy numbers can be achieved with many types of plasmid vectors.

The PCR method was developed by

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