Question 1

A 30* objective and a 20* ocular produce a total magnification of

Accepted Answer

A) 230*. 
B) 320*. 
C) 50*. 
D) 600*. 
A) 230*. 
B) 320*. 
C) 50*. 
D) 600*. D

Question 2

The Gram-staining procedure is an example of ________________________.

Accepted Answer

A) simple staining 
B) negative staining 
C) differential staining 
D) fluorescent staining 
A) simple staining 
B) negative staining 
C) differential staining 
D) fluorescent staining C

Question 3

An instrument that magnifies slight differences in the refractive index of cell structures is called a (n)__________ microscope.

Accepted Answer

A) phase-contrast 
B) electron 
C) fluorescence 
D) densitometric 
A) phase-contrast 
B) electron 
C) fluorescence 
D) densitometric A

Question 4

You are researching the structure of a transmembrane protein.Which type of microscopy would provide you the best view of this protein?

Accepted Answer

A) Bright field microscopy 
B) Scanning electron microscopy 
C) Transmission electron microscopy 
D) Atomic force microscopy 
A) Bright field microscopy 
B) Scanning electron microscopy 
C) Transmission electron microscopy 
D) Atomic force microscopy

Question 5

The __________ is the distance between the center of a lens and the point at which it focuses parallel beams of light.

Accepted Answer

The answer of The __________ is the distance between the...

Question 6

Mordants increase the binding between a stain and specimen.

Accepted Answer

A) True 
 B)False

Question 7

Thin films of bacteria that have been air-dried onto a glass microscope slide are called __________.

Accepted Answer

The answer of Thin films of bacteria that have been...

Question 8

Negative staining with India ink can be used to reveal the presence of capsules that surround bacterial cells.

Accepted Answer

A) True 
 B)False

Question 9

The _________________ microscope is capable of atomic resolution of specimens,even when they are immersed in water.

Accepted Answer

The answer of The _________________ microscope is capable of atomic...

Question 10

A light microscope with an objective lens numerical aperture of 0.65 is capable of allowing two objects 400 nm apart to be distinguished when using light with a wavelength of 420 nm.

Accepted Answer

A) True 
 B)False

Question 11

The instrument that produces a bright image of the specimen against a dark background is called a (n)__________ microscope.

Accepted Answer

A) phase-contrast 
B) electron 
C) bright-field 
D) dark-field 
A) phase-contrast 
B) electron 
C) bright-field 
D) dark-field

Question 12

It was possible to view viruses only after the invention of the electron microscope because they are too small to be seen with a light microscope.

Accepted Answer

A) True 
 B)False

Question 13

Immersion oil can be used to increase the resolution achieved with some microscope lenses because it increases the __________ between the specimen and the objective lens.

Accepted Answer

A) optical density 
B) refractive index 
C) optical density and refractive index 
D) neither optical density nor refractive index 
A) optical density 
B) refractive index 
C) optical density and refractive index 
D) neither optical density nor refractive index

Question 14

Confocal microscopes,in combination with specialized computer software,can be used to create three-dimensional images of cell structures.

Accepted Answer

A) True 
 B)False

Question 15

In the Gram-staining procedure,the counterstain is

Accepted Answer

A) iodine. 
B) safranin. 
C) crystal violet. 
D) alcohol. 
A) iodine. 
B) safranin. 
C) crystal violet. 
D) alcohol.

Question 16

In order to stain flagella so that they may be readily observed by light microscopy,it is usually necessary to increase their thickness.

Accepted Answer

A) True 
 B)False

Question 17

Regions of a specimen with higher electron density scatter ___________ electrons and,therefore,appear __________ in the image projected onto the screen of a transmission electron microscope.

Accepted Answer

A) more; lighter 
B) more; darker 
C) fewer; darker 
D) fewer; lighter 
A) more; lighter 
B) more; darker 
C) fewer; darker 
D) fewer; lighter

Question 18

It is possible to build a light microscope capable of 10,000* magnification,but the image would not be sharp because resolution is independent of magnification.

Accepted Answer

A) True 
 B)False

Question 19

The __________ is the point at which a lens focuses parallel beams of light.

Accepted Answer

The answer of The __________ is the point at which...

Question 20

Prior to staining,smears of microorganisms are heat-fixed in order to

Accepted Answer

A) allow eventual visualization of internal structures. 
B) ensure removal of dust particles from the slide surface. 
C) attach it firmly to the slide. 
D) create small pores in cells that facilitates binding of stain to cell structures. 
A) allow eventual visualization of internal structures. 
B) ensure removal of dust particles from the slide surface. 
C) attach it firmly to the slide. 
D) create small pores in cells that facilitates binding of stain to cell structures.

Exam 2: Microscopy

A 30* objective and a 20* ocular produce a total magnification of

The Gram-staining procedure is an example of ________________________.

An instrument that magnifies slight differences in the refractive index of cell structures is called a (n)__________ microscope.

You are researching the structure of a transmembrane protein.Which type of microscopy would provide you the best view of this protein?

The __________ is the distance between the center of a lens and the point at which it focuses parallel beams of light.

Mordants increase the binding between a stain and specimen.

Thin films of bacteria that have been air-dried onto a glass microscope slide are called __________.

Negative staining with India ink can be used to reveal the presence of capsules that surround bacterial cells.

The _________________ microscope is capable of atomic resolution of specimens,even when they are immersed in water.

A light microscope with an objective lens numerical aperture of 0.65 is capable of allowing two objects 400 nm apart to be distinguished when using light with a wavelength of 420 nm.

The instrument that produces a bright image of the specimen against a dark background is called a (n)__________ microscope.

It was possible to view viruses only after the invention of the electron microscope because they are too small to be seen with a light microscope.

Immersion oil can be used to increase the resolution achieved with some microscope lenses because it increases the __________ between the specimen and the objective lens.

Confocal microscopes,in combination with specialized computer software,can be used to create three-dimensional images of cell structures.

In the Gram-staining procedure,the counterstain is

In order to stain flagella so that they may be readily observed by light microscopy,it is usually necessary to increase their thickness.

Regions of a specimen with higher electron density scatter ___________ electrons and,therefore,appear __________ in the image projected onto the screen of a transmission electron microscope.

It is possible to build a light microscope capable of 10,000* magnification,but the image would not be sharp because resolution is independent of magnification.

The __________ is the point at which a lens focuses parallel beams of light.

Prior to staining,smears of microorganisms are heat-fixed in order to

The Evolution of Microorganisms and Microbiology

Bacterial Cell Structure

Archaeal Cell Structure

Eukaryotic Cell Structure

Viruses and Other Acellular Infectious Agents

Microbial Growth

Control of Microorganisms in the Environment

Antimicrobial Chemotherapy

Introduction to Metabolism

Catabolism: Energy Release and Conservation

Anabolism: the Use of Energy in Biosynthesis

Bacterial Genome Replication and Expression

Regulation of Bacterial Cellular Processes

Eukaryotic and Archaeal Genome Replication and Expression

Mechanisms of Genetic Variation

Recombinant DNA Technology

Microbial Genomics

Microbial Taxonomy and the Evolution of Diversity

Archaea

The Deinococci, Mollicutes, and Nonproteobacterial Gram-Negative Bacteria

The Proteobacteria

Firmicutes: The Low G-C Gram-Positive Bacteria

Actinobacteria: the High G-C Gram-Positive Bacteria

Protists

Fungi Eumycota

Viruses

Biogeochemical Cycling and Global Climate Change

Methods in Microbial Ecology

Microorganisms in Marine and Freshwater Ecosystems

Microorganisms in Terrestrial Ecosystems

Microbial Interactions

Innate Host Resistance

Adaptive Immunity

Pathogenicity and Infection

Clinical Microbiology and Immunology

Epidemiology and Public Health Microbiology

Human Diseases Caused by Viruses and Prions

Human Diseases Caused by Bacteria

Human Diseases Caused by Fungi and Protists

Microbiology of Food

Biotechnology and Industrial Microbiology

Applied Environmental Microbiology

Filters

Regions of a specimen with higher electron density scatter _ electrons and,therefore,appear in the image projected onto the screen of a transmission electron microscope.