Question 1

Small internal cell structures are best visualized with a

Accepted Answer

A) light microscope. 
B) dark-field microscope. 
C) transmission electron microscope. 
D) flagellar microscope. 
A) light microscope. 
B) dark-field microscope. 
C) transmission electron microscope. 
D) flagellar microscope.

Question 2

Negative staining facilitates the visualization of bacterial capsules that are intensely stained by the procedure.

Accepted Answer

A) True 
 B)False

Question 3

Confocal microscopes exhibit improved contrast and resolution by

Accepted Answer

A) illumination of a large area of the specimen. 
B) blocking out stray light with an aperture located above the objective lens. 
C) use of light at longer wavelengths. 
D) use of ultraviolet light to illuminate the specimen. 
A) illumination of a large area of the specimen. 
B) blocking out stray light with an aperture located above the objective lens. 
C) use of light at longer wavelengths. 
D) use of ultraviolet light to illuminate the specimen.

Question 4

If you forgot the decolorization step while performing a Gram stain,which outcome would you expect?

Accepted Answer

A) Gram-positive bacteria would stain pink. 
B) Gram-negative bacteria would stain purple. 
C) Gram-negative bacteria would be unstained. 
D) Gram-positive bacteria would be unstained. 
A) Gram-positive bacteria would stain pink. 
B) Gram-negative bacteria would stain purple. 
C) Gram-negative bacteria would be unstained. 
D) Gram-positive bacteria would be unstained.

Question 5

A specimen has been prepared for viewing with a transmission electron microscope,using uranyl acetate as a negative stain.The area stained by the uranyl acetate will be ________________ electron dense compared to specimen itself.

Accepted Answer

A) more 
B) less 
C) equally 
A) more 
B) less 
C) equally

Question 6

A substage condenser is used to focus light onto the specimen,which increases the resolution of a light microscope.

Accepted Answer

A) True 
 B)False

Question 7

Scanning electron microscopy is most often used to reveal

Accepted Answer

A) surface structures. 
B) internal structures. 
C) both surface and internal structures simultaneously. 
D) either surface or internal structures,but not simultaneously. 
A) surface structures. 
B) internal structures. 
C) both surface and internal structures simultaneously. 
D) either surface or internal structures,but not simultaneously.

Question 8

After the primary stain has been added but before the decolorizer has been used,gram-positive organisms are stained __________ and gram-negative organisms are stained __________.

Accepted Answer

A) purple; purple 
B) purple; colorless 
C) purple; pink 
D) pink; pink 
A) purple; purple 
B) purple; colorless 
C) purple; pink 
D) pink; pink

Question 9

Because transmission electron microscopy uses electrons rather than light,it is not necessary to stain biological specimens before observing them.

Accepted Answer

A) True 
 B)False

Question 10

After the secondary stain has been added,gram-positive organisms are stained __________ and gram-negative organisms are stained __________.

Accepted Answer

A) purple; purple 
B) purple; colorless 
C) purple; pink 
D) pink; pink 
A) purple; purple 
B) purple; colorless 
C) purple; pink 
D) pink; pink

Question 11

The __________ is the distance between the specimen and the objective lens when the specimen is in focus.

Accepted Answer

The answer of The __________ is the distance between the...

Question 12

Immersion oil is used to prevent a specimen from drying out.

Accepted Answer

A) True 
 B)False

Question 13

In order to view a specimen with a total magnification of 400*,a __________ objective must be used if the ocular is *.

Accepted Answer

The answer of In order to view a specimen with...

Question 14

Resolution improves when the wavelength of the illuminating light decreases.

Accepted Answer

A) True 
 B)False

Question 15

__________ is the process by which internal and external structures of cells and organisms are preserved and maintained in position.

Accepted Answer

The answer of __________ is the process by which internal...

Question 16

Basic dyes such as methylene blue bind to cellular molecules that are

Accepted Answer

A) hydrophobic. 
B) negatively charged. 
C) positively charged. 
D) aromatic. 
A) hydrophobic. 
B) negatively charged. 
C) positively charged. 
D) aromatic.

Question 17

The Gram-staining procedure is widely used because it allows rapid identification of a microorganism with little additional testing.

Accepted Answer

A) True 
 B)False

Question 18

__________ breaks frozen specimens along lines of greatest weakness,often down the middle of lipid bilayer membranes so that they may be observed by transmission electron microscopy.

Accepted Answer

The answer of __________ breaks frozen specimens along lines of...

Question 19

If the decolorizer is not left on long enough in the Gram-staining procedure,gram-positive organisms will be stained __________ and gram-negative organisms will be stained __________.

Accepted Answer

A) purple; purple 
B) purple; colorless 
C) purple; pink 
D) pink; pink 
A) purple; purple 
B) purple; colorless 
C) purple; pink 
D) pink; pink

Question 20

Immersion oil increases the amount of light entering the objective lens.

Accepted Answer

A) True 
 B)False

Exam 2: Microscopy

Small internal cell structures are best visualized with a

Negative staining facilitates the visualization of bacterial capsules that are intensely stained by the procedure.

Confocal microscopes exhibit improved contrast and resolution by

If you forgot the decolorization step while performing a Gram stain,which outcome would you expect?

A specimen has been prepared for viewing with a transmission electron microscope,using uranyl acetate as a negative stain.The area stained by the uranyl acetate will be ________________ electron dense compared to specimen itself.

A substage condenser is used to focus light onto the specimen,which increases the resolution of a light microscope.

Scanning electron microscopy is most often used to reveal

After the primary stain has been added but before the decolorizer has been used,gram-positive organisms are stained __________ and gram-negative organisms are stained __________.

Because transmission electron microscopy uses electrons rather than light,it is not necessary to stain biological specimens before observing them.

After the secondary stain has been added,gram-positive organisms are stained __________ and gram-negative organisms are stained __________.

The __________ is the distance between the specimen and the objective lens when the specimen is in focus.

Immersion oil is used to prevent a specimen from drying out.

In order to view a specimen with a total magnification of 400*,a __________ objective must be used if the ocular is *.

Resolution improves when the wavelength of the illuminating light decreases.

__________ is the process by which internal and external structures of cells and organisms are preserved and maintained in position.

Basic dyes such as methylene blue bind to cellular molecules that are

The Gram-staining procedure is widely used because it allows rapid identification of a microorganism with little additional testing.

__________ breaks frozen specimens along lines of greatest weakness,often down the middle of lipid bilayer membranes so that they may be observed by transmission electron microscopy.

If the decolorizer is not left on long enough in the Gram-staining procedure,gram-positive organisms will be stained __________ and gram-negative organisms will be stained __________.

Immersion oil increases the amount of light entering the objective lens.

The Evolution of Microorganisms and Microbiology

Bacterial Cell Structure

Archaeal Cell Structure

Eukaryotic Cell Structure

Viruses and Other Acellular Infectious Agents

Microbial Growth

Control of Microorganisms in the Environment

Antimicrobial Chemotherapy

Introduction to Metabolism

Catabolism: Energy Release and Conservation

Anabolism: the Use of Energy in Biosynthesis

Bacterial Genome Replication and Expression

Regulation of Bacterial Cellular Processes

Eukaryotic and Archaeal Genome Replication and Expression

Mechanisms of Genetic Variation

Recombinant DNA Technology

Microbial Genomics

Microbial Taxonomy and the Evolution of Diversity

Archaea

The Deinococci, Mollicutes, and Nonproteobacterial Gram-Negative Bacteria

The Proteobacteria

Firmicutes: The Low G-C Gram-Positive Bacteria

Actinobacteria: the High G-C Gram-Positive Bacteria

Protists

Fungi Eumycota

Viruses

Biogeochemical Cycling and Global Climate Change

Methods in Microbial Ecology

Microorganisms in Marine and Freshwater Ecosystems

Microorganisms in Terrestrial Ecosystems

Microbial Interactions

Innate Host Resistance

Adaptive Immunity

Pathogenicity and Infection

Clinical Microbiology and Immunology

Epidemiology and Public Health Microbiology

Human Diseases Caused by Viruses and Prions

Human Diseases Caused by Bacteria

Human Diseases Caused by Fungi and Protists

Microbiology of Food

Biotechnology and Industrial Microbiology

Applied Environmental Microbiology

Filters

After the primary stain has been added but before the decolorizer has been used,gram-positive organisms are stained and gram-negative organisms are stained .

After the secondary stain has been added,gram-positive organisms are stained and gram-negative organisms are stained .

In order to view a specimen with a total magnification of 400,a __________ objective must be used if the ocular is .

If the decolorizer is not left on long enough in the Gram-staining procedure,gram-positive organisms will be stained and gram-negative organisms will be stained .