Exam 3: Tools of the Laboratory: Methods for the Culturing of Microscopic Analysis of Microorganisms

Humans can see objects as small as 0.1 mm.Leeuwenhoek's best microscope could magnify objects to 300X.Which of the following would be invisible using Leeuwenhoek's microscope?

Who invented the first crude microscope by grinding glass?

Which of the following is produced by adding 1% to 5% agar to nutrient broth that is then boiled and cooled?

The _____ of the microscope holds and allows selection of the objective lenses.

Newly inoculated cultures must be _____ at a specific temperature and time to encourage growth.

What is the term for a culture made from one isolated colony?

Some microbes are not capable of growing on artificial media as pure cultures.

Comparing transmission electron microscopy with scanning electron microscopy,the following statement is true.

A nutrient medium that contains at least one ingredient that is not chemically defined is termed ______.

Robert Koch and his colleagues first used agar in bacteriological media.Why was this ingredient an improvement on previous materials used to culture bacteria?

The specimen preparation technique that is best for viewing cell motility is the ______.

A microbiologist inoculates Staphylococcus aureus into a culture medium.Following incubation,both Staphylococcus aureus and Staphylococcus epidermidis are determined to be growing in this culture.What is the most likely explanation?

A common medium for culturing fastidious bacteria is ______.

A selective medium contains one or more substances that inhibit growth of some microbes to facilitate the growth of other desired microbes.

What do the Gram stain,the acid-fast stain,and the endospore stain have in common?

The wavelength of light used and the numerical aperture governs ______.

Cells of Staphylococcus aureus are cocci measuring about 1 $\mu$ m in diameter.In a textbook image of S. aureus,each cell measures about 1 cm.The magnification of the image on the page is ______.

The bacterium Neisseria gonorrhoeae causes the sexually-transmitted infection gonorrhea.Although it can be isolated from the vagina,there are numerous normal biota in that location as well.To generate a pure culture of this pathogenic bacterium,the best choice is a(n)______.

Which type of microscope achieves the greatest resolution and highest magnification?

A nutrient medium that has all of its chemical components identified and their precise concentrations known and reproducible is termed ______.