Question 1

The enzyme required to attach the sticky ends of DNA and used when splicing DNA fragments into other DNA is ______.

Accepted Answer

A)  endonuclease 
B)  ligase 
C)  reverse transcriptase 
D)  helicase 
A)  endonuclease 
B)  ligase 
C)  reverse transcriptase 
D)  helicase B

Question 2

The study of genomes of a particular community is called ______.

Accepted Answer

A)  metabolomics 
B)  proteomics 
C)  genomology 
D)  genomics 
E)  metagenomics 
A)  metabolomics 
B)  proteomics 
C)  genomology 
D)  genomics 
E)  metagenomics E

Question 3

DNA polymerases used in PCR

Accepted Answer

A)  use an RNA template to make complementary DNA. 
B)  must remain active at very cold temperatures. 
C)  include Taq polymerases and Vent polymerase. 
D)  are labeled with fluorescent dyes. 
A)  use an RNA template to make complementary DNA. 
B)  must remain active at very cold temperatures. 
C)  include Taq polymerases and Vent polymerase. 
D)  are labeled with fluorescent dyes. C

Question 4

The commercial product Frostban consists of a genetically altered bacterium which prevents ice crystals from forming on plants,thereby reducing freezing of plants and financial distress to the farmers as a result of freezing weather.This product contains a strain of ______.

Accepted Answer

A)  Escherichia coli 
B)  Saccharomyces cerevisiae 
C)  Thermus aquaticus 
D)  Pseudomonas fluorescens 
E)  Pseudomonas syringae 
A)  Escherichia coli 
B)  Saccharomyces cerevisiae 
C)  Thermus aquaticus 
D)  Pseudomonas fluorescens 
E)  Pseudomonas syringae

Question 5

The process of introducing a needed,normal gene into human cells is called DNA mapping.

Accepted Answer

A) True 
 B)False

Question 6

Identification of unique DNA fingerprints relies on the presence of single nucleotide polymorphisms among samples.

Accepted Answer

A) True 
 B)False

Question 7

Fluorescent in situ hybridization (FISH)probes are applied to intact cells and observed microscopically for the presence and location of

Accepted Answer

A)  DNA. 
B)  proteins. 
C)  a specific genetic marker sequence. 
D)  recombinant DNA. 
E)  RNA. 
A)  DNA. 
B)  proteins. 
C)  a specific genetic marker sequence. 
D)  recombinant DNA. 
E)  RNA.

Question 8

Which of the following is a list of the materials required for PCR?

Accepted Answer

A)  Primers, Taq DNA polymerase, nucleotides 
B)  Primers, Taq RNA polymerase, nucleotides 
C)  Reverse transcriptase, Taq RNA polymerase, nucleotides 
D)  Reverse transcriptase, Taq DNA polymerase, nucleotides 
A)  Primers, Taq DNA polymerase, nucleotides 
B)  Primers, Taq RNA polymerase, nucleotides 
C)  Reverse transcriptase, Taq RNA polymerase, nucleotides 
D)  Reverse transcriptase, Taq DNA polymerase, nucleotides

Question 9

One reason the field of proteomics is continually evolving following the systematic study of an organism's genome is because

Accepted Answer

A)  it became apparent that while the organism's genome is relatively stable, protein expression in an organism is constantly changing. 
B)  the expression of proteins is fixed in each organism so sequencing the genome is essential to understanding phenotypes. 
C)  it became apparent that protein expression is relatively uniform, whereas the genome is constantly changing. 
D)  once the genome had been sequenced, all protein expression was understood. 
A)  it became apparent that while the organism's genome is relatively stable, protein expression in an organism is constantly changing. 
B)  the expression of proteins is fixed in each organism so sequencing the genome is essential to understanding phenotypes. 
C)  it became apparent that protein expression is relatively uniform, whereas the genome is constantly changing. 
D)  once the genome had been sequenced, all protein expression was understood.

Question 10

A technique that separates a readable pattern of DNA fragments is ______.

Accepted Answer

A)  genetic engineering 
B)  biotechnology 
C)  recombinant DNA 
D)  gel electrophoresis 
E)  gene probes 
A)  genetic engineering 
B)  biotechnology 
C)  recombinant DNA 
D)  gel electrophoresis 
E)  gene probes

Question 11

Which step in gene mapping involves transformation?

Accepted Answer

A)  Target DNA removed from cells and isolated 
B)  Cloning host receives plasmid 
C)  Target DNA and plasmid treated with the same restriction endonuclease 
D)  Desired protein is produced by cloning host 
E)  Gene is amplified by multiplication of cloning host 
A)  Target DNA removed from cells and isolated 
B)  Cloning host receives plasmid 
C)  Target DNA and plasmid treated with the same restriction endonuclease 
D)  Desired protein is produced by cloning host 
E)  Gene is amplified by multiplication of cloning host

Question 12

Which of the following is not true of vectors?

Accepted Answer

A)  They are easy to manipulate. 
B)  They can detect RNA in cells. 
C)  An origin of replication (ORI) is present. 
D)  They contain a gene for drug resistance. 
E)  They must accept DNA of desired size. 
A)  They are easy to manipulate. 
B)  They can detect RNA in cells. 
C)  An origin of replication (ORI) is present. 
D)  They contain a gene for drug resistance. 
E)  They must accept DNA of desired size.

Question 13

You want to identify Mycobacterium tuberculosis in a patient sputum sample.Which procedure would be most useful in this case?

Accepted Answer

A)  Whole genome sequencing 
B)  Polymerase chain reaction 
C)  DNA probe analysis 
D)  Microarray analysis 
A)  Whole genome sequencing 
B)  Polymerase chain reaction 
C)  DNA probe analysis 
D)  Microarray analysis

Question 14

EcoRI and HindIII are ______.

Accepted Answer

A)  palindromes 
B)  reverse transcriptases 
C)  restriction endonucleases 
D)  ligases 
E)  DNA polymerases 
A)  palindromes 
B)  reverse transcriptases 
C)  restriction endonucleases 
D)  ligases 
E)  DNA polymerases

Question 15

You have made a specific DNA probe that will bind to a key sequence on the DNA of Neisseria gonorrhoeae,allowing your company to market a gonorrhea test kit that can be used to identify the bacterium in genital tract specimens.However,upon testing it out against a known Neisseria gonorrhoeae culture,you find that it does not work.Which of the following is a possible explanation for this negative result?

Accepted Answer

A)  You forgot to label the probe sequence with a reporter molecule. 
B)  You forgot to add the mRNA to the DNA probe sample. 
C)  You forgot to digest the probe with restriction endonucleases. 
D)  You forgot to incubate the test at the 75oC temperature required for hybridization. 
A)  You forgot to label the probe sequence with a reporter molecule. 
B)  You forgot to add the mRNA to the DNA probe sample. 
C)  You forgot to digest the probe with restriction endonucleases. 
D)  You forgot to incubate the test at the 75oC temperature required for hybridization.

Question 16

Before the advent of biotechnology,diabetics would use insulin harvested from cows.Now they receive human insulin produced through ______.

Accepted Answer

A)  gel electrophoresis 
B)  gene therapy 
C)  PCR 
D)  recombinant DNA technology 
A)  gel electrophoresis 
B)  gene therapy 
C)  PCR 
D)  recombinant DNA technology

Question 17

What type of DNA map gives locations and sizes of DNA sections?

Accepted Answer

A)  Linkage 
B)  Sequence 
C)  Physical 
D)  Geographical 
E)  Chromosomal 
A)  Linkage 
B)  Sequence 
C)  Physical 
D)  Geographical 
E)  Chromosomal

Question 18

Each of the following are features of a cloning vector except

Accepted Answer

A)  origin of replication. 
B)  reverse transcriptases. 
C)  genetic markers used to screen for recombinants. 
D)  capacity for large inserts. 
E)  multiple cloning sites. 
A)  origin of replication. 
B)  reverse transcriptases. 
C)  genetic markers used to screen for recombinants. 
D)  capacity for large inserts. 
E)  multiple cloning sites.

Question 19

Any use of an organism's biochemical processes to create a product is referred to as ______.

Accepted Answer

A)  genetic engineering 
B)  biotechnology 
C)  recombinant DNA 
D)  gel electrophoresis 
E)  gene probes 
A)  genetic engineering 
B)  biotechnology 
C)  recombinant DNA 
D)  gel electrophoresis 
E)  gene probes

Question 20

Each of the following are features of a cloning host except

Accepted Answer

A)  quick generation time. 
B)  minimal growth requirements. 
C)  mapped genome. 
D)  pathogenicity. 
E)  transformable. 
A)  quick generation time. 
B)  minimal growth requirements. 
C)  mapped genome. 
D)  pathogenicity. 
E)  transformable.

Exam 10: Genetic Engineering and Recombinant Dna

The enzyme required to attach the sticky ends of DNA and used when splicing DNA fragments into other DNA is ______.

The study of genomes of a particular community is called ______.

DNA polymerases used in PCR

The commercial product Frostban consists of a genetically altered bacterium which prevents ice crystals from forming on plants,thereby reducing freezing of plants and financial distress to the farmers as a result of freezing weather.This product contains a strain of ______.

The process of introducing a needed,normal gene into human cells is called DNA mapping.

Identification of unique DNA fingerprints relies on the presence of single nucleotide polymorphisms among samples.

Fluorescent in situ hybridization (FISH)probes are applied to intact cells and observed microscopically for the presence and location of

Which of the following is a list of the materials required for PCR?

One reason the field of proteomics is continually evolving following the systematic study of an organism's genome is because

A technique that separates a readable pattern of DNA fragments is ______.

Which step in gene mapping involves transformation?

Which of the following is not true of vectors?

You want to identify Mycobacterium tuberculosis in a patient sputum sample.Which procedure would be most useful in this case?

EcoRI and HindIII are ______.

Before the advent of biotechnology,diabetics would use insulin harvested from cows.Now they receive human insulin produced through ______.

What type of DNA map gives locations and sizes of DNA sections?

Each of the following are features of a cloning vector except

Any use of an organism's biochemical processes to create a product is referred to as ______.

Each of the following are features of a cloning host except

The Main Themes of Microbiology

The Chemistry of Biology

Tools of the Laboratory: Methods for the Culturing of Microscopic Analysis of Microorganisms

Bacteria and Archaea

Eukaryotic Cells and Microorganisms

An Introduction to the Viruses

Host Defenses II: Specific Immunity and Immunization

Microbial Metabolism: The Chemical Crossroads of Life

Microbial Genetics

Physical and Chemical Control of Microbes

Antimicrobial Treatment

Microbe-Human Interactions: Health and Disease

Host Defenses I: Overview and Nonspecific Defenses

Host Defenses II: Specific Immunity and Immunization

Disorders in Immunity

Diagnosing Infections

Infectious Diseases Affecting the Skin and Eyes

Infectious Diseases Affecting the Nervous System

Infectious Diseases Affecting the Cardiovascular and Lymphatic Systems

Infectious Diseases Affecting the Respiratory System

Infectious Diseases Affecting the Gastrointestinal Tract

Infectious Diseases Affecting the Genitourinary System

Microbes and the Environment

Applied Microbiology and Food and Water Safety

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