Question 1

Mixed cultures are also referred to as contaminated cultures.

Accepted Answer

A) True 
 B)False

Question 2

Mannitol salt agar is selective for organisms in which bacterial genus?

Accepted Answer

A)  Salmonella 
B)  Staphylococcus 
C)  Escherichia 
D)  Neisseria 
E)  Streptococcus 
A)  Salmonella 
B)  Staphylococcus 
C)  Escherichia 
D)  Neisseria 
E)  Streptococcus

Question 3

Which type of microscope cannot image live specimens?

Accepted Answer

A)  Differential interference 
B)  Bright-field 
C)  Electron 
D)  Dark-field 
E)  Phase-contrast 
A)  Differential interference 
B)  Bright-field 
C)  Electron 
D)  Dark-field 
E)  Phase-contrast

Question 4

Table 3.6 in your textbook uses Paramecium to illustrate several types of light microscopy.Each image is magnified 230X and measures about 4 cm.The actual size of a Paramecium is about ______.

Accepted Answer

A)  920 $\mu$m 
B)  0.2 $\mu$m 
C) 175 $\mu$m 
D)  57.5 $\mu$m 
E)  4,000 $\mu$m 
A)  920 $\mu$m 
B)  0.2 $\mu$m 
C) 175 $\mu$m 
D)  57.5 $\mu$m 
E)  4,000 $\mu$m

Question 5

All of the following are diameters of cells that would be resolved in a microscope with a limit of resolution of 0.2 µm except ______.

Accepted Answer

A)  0.2 mm 
B)  0.1 µm 
C)  2.0 µm 
D)  0.2 µm 
A)  0.2 mm 
B)  0.1 µm 
C)  2.0 µm 
D)  0.2 µm

Question 6

All of the following pertain to the fluorescence microscope except

Accepted Answer

A)  it is a type of compound microscope. 
B)  it is used to diagnose certain infections. 
C)  it uses electrons to produce a specimen image. 
D)  it requires the use of dyes like acridine or fluorescein. 
E)  it requires an ultraviolet radiation source. 
A)  it is a type of compound microscope. 
B)  it is used to diagnose certain infections. 
C)  it uses electrons to produce a specimen image. 
D)  it requires the use of dyes like acridine or fluorescein. 
E)  it requires an ultraviolet radiation source.

Question 7

The purpose of staining cells on a microscope slide is to ______.

Accepted Answer

A)  secure them to the slide 
B)  kill them 
C)  observe motility 
D)  enlarge the cells 
E)  add contrast to see them better 
A)  secure them to the slide 
B)  kill them 
C)  observe motility 
D)  enlarge the cells 
E)  add contrast to see them better

Question 8

The Five I's of studying microorganisms include all of the following except ______.

Accepted Answer

A)  identification 
B)  isolation 
C)  incubation 
D)  infection 
E)  inoculation 
A)  identification 
B)  isolation 
C)  incubation 
D)  infection 
E)  inoculation

Question 9

Which type of microscope bombards a whole,metal-coated specimen with electrons moving back and forth over it?

Accepted Answer

A)  Transmission electron 
B)  Differential interference contrast 
C)  Scanning electron 
D)  Phase-contrast 
E)  Fluorescence 
A)  Transmission electron 
B)  Differential interference contrast 
C)  Scanning electron 
D)  Phase-contrast 
E)  Fluorescence

Question 10

A microbiology student with a visual disability is viewing a sample of Bacillus endospores in a phase contrast microscope that is linked to an iPad.By "stretching" the image on the iPad screen,the student can increase the apparent size of the endospores.How does this manipulation affect resolution and contrast? (No software is being used to alter the image!)

Accepted Answer

A)  Only the magnification can be increased; resolution and contrast are unchanged. 
B)  Contrast will improve because the microscope is phase-contrast. 
C)  Both magnification and resolution increase; contrast is unchanged. 
D)  Resolution and magnification will decrease because the screen is not as good as the human eye. 
A)  Only the magnification can be increased; resolution and contrast are unchanged. 
B)  Contrast will improve because the microscope is phase-contrast. 
C)  Both magnification and resolution increase; contrast is unchanged. 
D)  Resolution and magnification will decrease because the screen is not as good as the human eye.

Question 11

Which of the following is not a benefit of agar as a solid medium?

Accepted Answer

A)  Holds moisture 
B)  Flexibility 
C)  Solid at room temperature 
D)  Can be inoculated and poured at a temperature that is not harmful 
E)  Easily digested by most microbes 
A)  Holds moisture 
B)  Flexibility 
C)  Solid at room temperature 
D)  Can be inoculated and poured at a temperature that is not harmful 
E)  Easily digested by most microbes

Question 12

Bacteria that require special growth factors and complex nutrients are termed ______.

Accepted Answer

A)  fermentative 
B)  hemolytic 
C)  fastidious 
D)  aerobic 
E)  anaerobic 
A)  fermentative 
B)  hemolytic 
C)  fastidious 
D)  aerobic 
E)  anaerobic

Question 13

The procedures for culturing a microorganism require the use of a microscope.

Accepted Answer

A) True 
 B)False

Question 14

The term that refers to the purposeful addition of microorganisms into a laboratory nutrient medium is ______.

Accepted Answer

A)  inoculation 
B)  contamination 
C)  infection 
D)  immunization 
E)  isolation 
A)  inoculation 
B)  contamination 
C)  infection 
D)  immunization 
E)  isolation

Question 15

A microbiologist must culture a patient's feces for intestinal pathogens.Which of the following components would likely be present in a selective medium for this purpose?

Accepted Answer

A)  Thioglycolic acid 
B)  Sheep red blood cells 
C)  Bile salts 
D)  Peptone 
E)  NaCl 
A)  Thioglycolic acid 
B)  Sheep red blood cells 
C)  Bile salts 
D)  Peptone 
E)  NaCl

Question 16

Which type of medium distinguishes different types of microorganisms based on an observable change in the colonies or in the medium?

Accepted Answer

A)  Enriched 
B)  Enumeration 
C)  Selective 
D)  Reducing 
E)  Differential 
A)  Enriched 
B)  Enumeration 
C)  Selective 
D)  Reducing 
E)  Differential

Question 17

Magnification is achieved in a compound microscope through the initial magnification of the specimen by the _____ lens.This image is then projected to the _____ lens that will further magnify the specimen to form a virtual image received by the eye.

Accepted Answer

A)  ocular; oil immersion 
B)  scanning; objective 
C)  ocular; objective 
D)  objective; ocular 
A)  ocular; oil immersion 
B)  scanning; objective 
C)  ocular; objective 
D)  objective; ocular

Question 18

Eosin-methylene blue agar,or EMB,is a commonly used bacteriological medium for growing gram-negative bacteria from complex environments.It contains two dyes,eosin and methylene blue,as well as the sugar lactose.Eosin is a pH indicator that changes color when the medium is acidic,while methylene blue inhibits the growth of most gram-positive bacteria.If an organism consumes lactose,acid will be produced,causing the bacterial colonies to change color. Based upon this description,this medium is ______.

Accepted Answer

A)  reducing 
B)  for enrichment 
C)  selective 
D)  differential and selective 
E)  differential 
A)  reducing 
B)  for enrichment 
C)  selective 
D)  differential and selective 
E)  differential

Question 19

If a microbiologist is studying a specimen at a total magnification of 950X,what is the magnifying power of the objective lens if the ocular lens is 10X?

Accepted Answer

A)  95X 
B)  940X 
C)  950X 
D)  9500X 
A)  95X 
B)  940X 
C)  950X 
D)  9500X

Question 20

Which term refers to the microscope's ability to show two separate objects as discrete and distinct?

Accepted Answer

A)  Contrast 
B)  Magnification 
C)  Refraction 
D)  Resolution 
A)  Contrast 
B)  Magnification 
C)  Refraction 
D)  Resolution

Exam 3: Tools of the Laboratory: Methods for the Culturing of Microscopic Analysis of Microorganisms

Mixed cultures are also referred to as contaminated cultures.

Mannitol salt agar is selective for organisms in which bacterial genus?

Which type of microscope cannot image live specimens?

Table 3.6 in your textbook uses Paramecium to illustrate several types of light microscopy.Each image is magnified 230X and measures about 4 cm.The actual size of a Paramecium is about ______.

All of the following are diameters of cells that would be resolved in a microscope with a limit of resolution of 0.2 µm except ______.

All of the following pertain to the fluorescence microscope except

The purpose of staining cells on a microscope slide is to ______.

The Five I's of studying microorganisms include all of the following except ______.

Which type of microscope bombards a whole,metal-coated specimen with electrons moving back and forth over it?

Which of the following is not a benefit of agar as a solid medium?

Bacteria that require special growth factors and complex nutrients are termed ______.

The procedures for culturing a microorganism require the use of a microscope.

The term that refers to the purposeful addition of microorganisms into a laboratory nutrient medium is ______.

A microbiologist must culture a patient's feces for intestinal pathogens.Which of the following components would likely be present in a selective medium for this purpose?

Which type of medium distinguishes different types of microorganisms based on an observable change in the colonies or in the medium?

Magnification is achieved in a compound microscope through the initial magnification of the specimen by the _____ lens.This image is then projected to the _____ lens that will further magnify the specimen to form a virtual image received by the eye.

If a microbiologist is studying a specimen at a total magnification of 950X,what is the magnifying power of the objective lens if the ocular lens is 10X?

Which term refers to the microscope's ability to show two separate objects as discrete and distinct?

The Main Themes of Microbiology

The Chemistry of Biology

Bacteria and Archaea

Eukaryotic Cells and Microorganisms

An Introduction to the Viruses

Host Defenses II: Specific Immunity and Immunization

Microbial Metabolism: The Chemical Crossroads of Life

Microbial Genetics

Genetic Engineering and Recombinant Dna

Physical and Chemical Control of Microbes

Antimicrobial Treatment

Microbe-Human Interactions: Health and Disease

Host Defenses I: Overview and Nonspecific Defenses

Host Defenses II: Specific Immunity and Immunization

Disorders in Immunity

Diagnosing Infections

Infectious Diseases Affecting the Skin and Eyes

Infectious Diseases Affecting the Nervous System

Infectious Diseases Affecting the Cardiovascular and Lymphatic Systems

Infectious Diseases Affecting the Respiratory System

Infectious Diseases Affecting the Gastrointestinal Tract

Infectious Diseases Affecting the Genitourinary System

Microbes and the Environment

Applied Microbiology and Food and Water Safety

Filters

Magnification is achieved in a compound microscope through the initial magnification of the specimen by the _ lens.This image is then projected to the _ lens that will further magnify the specimen to form a virtual image received by the eye.