Exam 3: Tools of the Laboratory: Methods for the Culturing of Microscopic Analysis of Microorganisms

The type of microscopy in which you would observe brightly illuminated specimens against a black background is ______.

Following a properly-performed Gram stain,gram-positive bacteria will appear as pink or red cells.

Which method often results in colonies developing down throughout the agar along with some colonies on the surface?

Which type of microscopy transforms the subtle variations in cell density into differences in light intensity,allowing for increased internal detail without staining?

A medium that is gel-like has less agar in it compared to a solid medium.

Your microscope has a resolving power of 0.15 $\mu$ m.The specimen that you will be observing has bacterial cocci the size of 0.1 $\mu$ m in diameter.Which of the following statements is correct?

Scattering of peripheral light rays through the glass slide under very high magnification can be prevented by ______.

A differential interference contrast microscope uses dyes to give colored,three-dimensional images.

Thioglycolate medium contains the following: yeast extract,pancreatic digest of casein,glucose,L-cysteine,sodium chloride,sodium thioglycolate,and agar.It is considered a complex medium because

Which of the following is essential for the development of discrete,isolated colonies?

Which type of medium would be the best choice when shipping a sample of bacteria from a clinic to a laboratory for testing?

Fixed smears of specimens are required to perform the Gram stain or the endospore stain.

A scanning confocal microscope

The Gram staining procedure is best described as a ______ staining technique.

Basic dyes are

Differential media result in which of the following observable characteristics?

Which type of microscope shows cells against a white background?

Which of the following is not an inoculating tool?

A reducing medium contains

A microbiologist makes a fixed smear of bacterial cells and stains it with methylene blue.All cells appear blue under the oil immersion lens.This is an example of ______.