Question 1

Proteins that undergo reversible changes in conformation when bound to another molecule are called

Accepted Answer

A) reversible peptides. 
B) allosteric proteins. 
C) inducer proteins. 
D) repressor proteins. 
E) allostatic proteins. 
A) reversible peptides. 
B) allosteric proteins. 
C) inducer proteins. 
D) repressor proteins. 
E) allostatic proteins. B

Question 2

The sigma factor that mediates a global heat shock response in E.coli is

Accepted Answer

A) sigma 70. 
B) sigma 32. 
C) sigma 34. 
D) sigma 72. 
E) sigma 36. 
A) sigma 70. 
B) sigma 32. 
C) sigma 34. 
D) sigma 72. 
E) sigma 36. B

Question 3

The transition from transcriptional initiation to elongation involves

Accepted Answer

A) binding of sigma factor. 
B) release of sigma factor. 
C) release of RNA polymerase from DNA. 
D) binding of RNA polymerase to DNA. 
E) binding of rho factor. 
A) binding of sigma factor. 
B) release of sigma factor. 
C) release of RNA polymerase from DNA. 
D) binding of RNA polymerase to DNA. 
E) binding of rho factor. B

Question 4

In the regulation of the trp operon, tryptophan acts as a

Accepted Answer

A) repressor. 
B) attenuator. 
C) activator. 
D) corepressor. 
E) operator. 
A) repressor. 
B) attenuator. 
C) activator. 
D) corepressor. 
E) operator.

Question 5

What is one possible advantage for targeting quorum sensing mechanisms for antibiotic delveopment?

Accepted Answer

A) There is no selective advantage for cells lacking quorum sensing mechanisms 
B) There is a selective advantage to those cells lacking a quorum sensing system. 
C) Quorum sensing mechanisms are found in all pathogenic bacteria. 
D) Quorum sensing mechanisms are only found in human pathogens. 
A) There is no selective advantage for cells lacking quorum sensing mechanisms 
B) There is a selective advantage to those cells lacking a quorum sensing system. 
C) Quorum sensing mechanisms are found in all pathogenic bacteria. 
D) Quorum sensing mechanisms are only found in human pathogens.

Question 6

In

Accepted Answer

A) inactivation of certain repressor proteins by elevated temperature. 
B) denaturing of DNA in the promoters in the genes of heat sensitive proteins. 
C) synthesis of alternative sigma factors at high temperatures, which then regulate transcription of heat shock genes. 
D) increasing the promoter affinity of already existing polymerase sigma factors at high temperatures. 
E) coli, the heat shock response, switching off the synthesis of some proteins and switching on of different proteins, is mediated by 
A) inactivation of certain repressor proteins by elevated temperature. 
B) denaturing of DNA in the promoters in the genes of heat sensitive proteins. 
C) synthesis of alternative sigma factors at high temperatures, which then regulate transcription of heat shock genes. 
D) increasing the promoter affinity of already existing polymerase sigma factors at high temperatures. 
E) coli, the heat shock response, switching off the synthesis of some proteins and switching on of different proteins, is mediated by

Question 7

How do positive regulators stimulate initiation of transcription by RNA polymerase?

Accepted Answer

A) by allowing passage of the polymerase through the operator 
B) by binding to the polymerase and increasing its ability to initiate transcription 
C) by causing the helix to unwind in the operator allowing easier initiation 
D) by making the transcription start site more exposed to the polymerase 
A) by allowing passage of the polymerase through the operator 
B) by binding to the polymerase and increasing its ability to initiate transcription 
C) by causing the helix to unwind in the operator allowing easier initiation 
D) by making the transcription start site more exposed to the polymerase

Question 8

The scientists who proposed the operon theory are

Accepted Answer

A) Monod and Jacob. 
B) Watson and Crick. 
C) Hardy and Weinberg. 
D) Darwin and Mendel. 
E) Hershey and Chase. 
A) Monod and Jacob. 
B) Watson and Crick. 
C) Hardy and Weinberg. 
D) Darwin and Mendel. 
E) Hershey and Chase.

Question 9

How do negative regulators such as the lac repressor prevent RNA polymerase from initiating transcription?

Accepted Answer

A) by blocking passage of the polymerase through the operator 
B) by forming a loop in the operator that restricts the passage of the polymerase 
C) by physically blocking the DNA binding site of RNA polymerase 
D) by binding to the polymerase, thus preventing its binding 
A) by blocking passage of the polymerase through the operator 
B) by forming a loop in the operator that restricts the passage of the polymerase 
C) by physically blocking the DNA binding site of RNA polymerase 
D) by binding to the polymerase, thus preventing its binding

Question 10

A single DNA unit that enables the simultaneous regulation of more than one coding region in response to environmental changes is called

Accepted Answer

A) promoter. 
B) operator. 
C) regulator. 
D) inducer. 
E) operon. 
A) promoter. 
B) operator. 
C) regulator. 
D) inducer. 
E) operon.

Question 11

Which statement is true regarding global gene regulation in bacteria?

Accepted Answer

A) Sigma factors are not involved in gene regulation, just attachment of the polymerase to the promoter. 
B) Alternative sigma factors recognizing different promoters carry out global gene regulation. 
C) All promoters are recognized by all sigma factors. 
D) coli cells devote more energy to the production of ribosomes during stress so that global gene regulation can occur. 
A) Sigma factors are not involved in gene regulation, just attachment of the polymerase to the promoter. 
B) Alternative sigma factors recognizing different promoters carry out global gene regulation. 
C) All promoters are recognized by all sigma factors. 
D) coli cells devote more energy to the production of ribosomes during stress so that global gene regulation can occur.

Question 12

The critical step in the regulation of most bacterial genes occurs _____________.

Accepted Answer

A) during transcription. 
B) at release of mRNA. 
C) during ranslation. 
D) post-translationally. 
A) during transcription. 
B) at release of mRNA. 
C) during ranslation. 
D) post-translationally.

Question 13

V.fischeri senses the desity of V.fisheri cells in the environment by

Accepted Answer

A) secretion of a molecule that can then internalized and bound to a receptor in the cytoplasm. 
B) cell surface receptors that interact with other cell surface receptors on other cells. 
C) a cell surface receptor that binds to a molecule secreted by other cells present in the external environment. 
D) a cytoplasmic receptor that binds to the lux C protein. 
A) secretion of a molecule that can then internalized and bound to a receptor in the cytoplasm. 
B) cell surface receptors that interact with other cell surface receptors on other cells. 
C) a cell surface receptor that binds to a molecule secreted by other cells present in the external environment. 
D) a cytoplasmic receptor that binds to the lux C protein.

Question 14

What is an advantage of using the lac regulatory regions to control the expression of exogenous proteins in bacterial cells?

Accepted Answer

A) Expression can be controlled by the addition of an inducer to the media. 
B) It works in both bacterial and mammalian cells. 
C) By using this regulatory region eukaryotic genomic DNA can be used for expression in bacterial cells. 
D) Host regulatory mechanisms are not effective when this regulatory regions are cloned into a plasmid. 
A) Expression can be controlled by the addition of an inducer to the media. 
B) It works in both bacterial and mammalian cells. 
C) By using this regulatory region eukaryotic genomic DNA can be used for expression in bacterial cells. 
D) Host regulatory mechanisms are not effective when this regulatory regions are cloned into a plasmid.

Question 15

Proteins that have their activity regulated by binding of another molecule at a site other than the active site are termed

Accepted Answer

A) enzymes. 
B) allosteric proteins. 
C) regulatory proteins. 
D) activator proteins. 
E) inhibitory proteins. 
A) enzymes. 
B) allosteric proteins. 
C) regulatory proteins. 
D) activator proteins. 
E) inhibitory proteins.

Question 16

What is the relationship between the promoters for anti-sense RNA and their targets?

Accepted Answer

A) The promoters are oriented so that they transcribe opposite strands of the same piece of DNA. 
B) The promoters are found in diffferent chromosomal regions but are controlled by the same sigma factors. 
C) The sense and antisense RNAs use the same promoter, both RNAs are produced from a single transcript that is processed.. 
D) The sense and antisense RNAs are trasncribed using the same promoter that is bidirectional. 
A) The promoters are oriented so that they transcribe opposite strands of the same piece of DNA. 
B) The promoters are found in diffferent chromosomal regions but are controlled by the same sigma factors. 
C) The sense and antisense RNAs use the same promoter, both RNAs are produced from a single transcript that is processed.. 
D) The sense and antisense RNAs are trasncribed using the same promoter that is bidirectional.

Question 17

Which of the following choices is NOT a correct description of a reporter gene?

Accepted Answer

A) a protein-encoding gene whose expression is easily quantifiable. 
B) often fused to another protein to assess its activity. 
C) often fused to another gene's regulatory region to determine control. 
D) None of the choices is correct. 
A) a protein-encoding gene whose expression is easily quantifiable. 
B) often fused to another protein to assess its activity. 
C) often fused to another gene's regulatory region to determine control. 
D) None of the choices is correct.

Question 18

Anabolic pathways involved in the synthesis of essential molecules are usually regulated by

Accepted Answer

A) end product of the pathway. 
B) substrate of the pathway. 
C) other metabolites that are limiting. 
D) None of the choices is correct. 
A) end product of the pathway. 
B) substrate of the pathway. 
C) other metabolites that are limiting. 
D) None of the choices is correct.

Question 19

A common amino acid motif found in many of the polypeptides that function as repressors is

Accepted Answer

A) Leucine zipper motif. 
B) Zinc finger motif. 
C) Helix-turn-helix motif. 
D) Helix-loop-helix motif. 
A) Leucine zipper motif. 
B) Zinc finger motif. 
C) Helix-turn-helix motif. 
D) Helix-loop-helix motif.

Question 20

Initiation of transcription by RNA polymerase involves the binding of which of the following subunits to the core enzyme?

Accepted Answer

A) delta 
B) sigma 
C) gamma 
D) alpha 
E) zeta 
A) delta 
B) sigma 
C) gamma 
D) alpha 
E) zeta

Exam 15: Gene Regulation in Prokaryotes

Proteins that undergo reversible changes in conformation when bound to another molecule are called

The sigma factor that mediates a global heat shock response in E.coli is

The transition from transcriptional initiation to elongation involves

In the regulation of the trp operon, tryptophan acts as a

What is one possible advantage for targeting quorum sensing mechanisms for antibiotic delveopment?

In

How do positive regulators stimulate initiation of transcription by RNA polymerase?

The scientists who proposed the operon theory are

How do negative regulators such as the lac repressor prevent RNA polymerase from initiating transcription?

A single DNA unit that enables the simultaneous regulation of more than one coding region in response to environmental changes is called

Which statement is true regarding global gene regulation in bacteria?

The critical step in the regulation of most bacterial genes occurs _____________.

V.fischeri senses the desity of V.fisheri cells in the environment by

What is an advantage of using the lac regulatory regions to control the expression of exogenous proteins in bacterial cells?

Proteins that have their activity regulated by binding of another molecule at a site other than the active site are termed

What is the relationship between the promoters for anti-sense RNA and their targets?

Which of the following choices is NOT a correct description of a reporter gene?

Anabolic pathways involved in the synthesis of essential molecules are usually regulated by

A common amino acid motif found in many of the polypeptides that function as repressors is

Initiation of transcription by RNA polymerase involves the binding of which of the following subunits to the core enzyme?

Genetics: The Study of Biological Information

Mendels Principles of Heredity

Extensions to Mendels Laws

The Chromosome Theory of Inheritance

Linkage, Recombination, and the Mapping of Genes on Chromosomes

DNA Structure, Replication, and Recombination

Anatomy and Function of a Gene: Dissection Through Mutation

Gene Expression: The Flow of Information From DNA to RNA to Protein

Digital Analysis of Genomes

Analyzing Genomic Information

The Eukaryotic Chromosome

Chromosomal Rearrangements and Changes in Chromosome Number

Bacterial Genetics

Organellar Inheritance

Gene Regulation in Eukaryotes

Manipulating the Genomes of Eukaryotes

The Genetic Analysis of Development

The Genetics of Cancer

Variation and Selection in Populations

Genetics of Complex Traits

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