Exam 3: Cell Structure and Function

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How could you use green fluorescent protein as a reporter gene to compare production of a specific protein in a cell culture when the culture is growing under different environmental conditions?

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Question 1

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The gene for green fluorescent protein could be fused to the gene that encodes the protein of interest. If that protein is produced when the cells are growing under specific conditions, the cells will also produce the green fluorescent protein. If that protein is not produced under certain growth conditions, the cells will not appear fluorescent green. The cell cultures could be grown using the different test conditions and the degree of fluorescence compared.

Which of the following is described as an attachment organelle that is a membrane-bound extension of the cytoplasm?

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Question 2

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__________ is impractical for proteins of a flexible nature.

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Question 3

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Describe how and why magnetotactic bacteria might be used in wastewater management.

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Question 4

The lipopolysaccharide of the outer membrane is of medical importance because it acts as a/an __________.

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Question 5

Two-dimensional polyacrylamide gel electrophoresis involves all of these EXCEPT:

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Question 6

The capsule polysaccharides form a slippery mucous layer that inhibits __________.

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Question 7

The bacterial flagellum is a helical protein filament whose __________ motor moves the cell in search of a more favorable environment.

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Question 8

Studying ribosome structure helps us to design new __________.

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Question 9

Caulobacter crescentus exists in two forms. What are they? Describe replication and what determines which form a cell takes. Why is the ability to exist in two forms advantageous?

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Question 10

All of the following are true of supercoiling in chromosomal DNA EXCEPT:

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Question 11

When designing an antibiotic, one should target what aspect of a prokaryote? Give at least two specific examples of drugs and their target.

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Question 12

The most highly expressed proteins in a cell are the proteins and the factors.

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Question 13

Specific membrane components, particularly __________, determine which substances are transported across the membrane.

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Question 14

If a prokaryotic intact ribosome is 70S, why are its subunits 30S and 50S, and not 30S and 40S?

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Question 15

The 70S prokaryotic ribosome is composed of a 30S and a __________ subunit.

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Question 16

Functions of the cytoplasmic membrane include all of the following EXCEPT:

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Question 17

Lipopolysaccharides are found in:

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Question 18

Describe four ways cells can be broken open in order to isolate the cellular components.

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Question 19

Polyamines are __________ charged when the pH is near neutral.

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Question 20

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Exam 3: Cell Structure and Function

How could you use green fluorescent protein as a reporter gene to compare production of a specific protein in a cell culture when the culture is growing under different environmental conditions?

Which of the following is described as an attachment organelle that is a membrane-bound extension of the cytoplasm?

__________ is impractical for proteins of a flexible nature.

Describe how and why magnetotactic bacteria might be used in wastewater management.

The lipopolysaccharide of the outer membrane is of medical importance because it acts as a/an __________.

Two-dimensional polyacrylamide gel electrophoresis involves all of these EXCEPT:

The capsule polysaccharides form a slippery mucous layer that inhibits __________.

The bacterial flagellum is a helical protein filament whose __________ motor moves the cell in search of a more favorable environment.

Studying ribosome structure helps us to design new __________.

Caulobacter crescentus exists in two forms. What are they? Describe replication and what determines which form a cell takes. Why is the ability to exist in two forms advantageous?

All of the following are true of supercoiling in chromosomal DNA EXCEPT:

When designing an antibiotic, one should target what aspect of a prokaryote? Give at least two specific examples of drugs and their target.

The most highly expressed proteins in a cell are the __________ proteins and the __________ factors.

Specific membrane components, particularly __________, determine which substances are transported across the membrane.

If a prokaryotic intact ribosome is 70S, why are its subunits 30S and 50S, and not 30S and 40S?

The 70S prokaryotic ribosome is composed of a 30S and a __________ subunit.

Functions of the cytoplasmic membrane include all of the following EXCEPT:

Lipopolysaccharides are found in:

Describe four ways cells can be broken open in order to isolate the cellular components.

Polyamines are __________ charged when the pH is near neutral.

Microbial Life: Origin and Discovery

Observing the Microbial Cell

Bacterial Culture, Growth, and Development

Environmental Influences and Control of Microbial Growth

Virus Structure and Function

Genomes and Chromosomes

Transcription, Translation, and Bioinformatics

Gene Transfer, Mutations, and Genome Evolution

Molecular Regulation

Viral Molecular Biology

Molecular Techniques and Biotechnology

Energetics and Catabolism

Respiration, Lithotrophy, and Photolysis

Biosynthesis

Food and Industrial Microbiology

Origins and Evolution

Bacterial Diversity

Archaeal Diversity

Eukaryotic Diversity

Microbial Ecology

Microbes and the Global Environment

Human Microbiota and Nonspecific Host Defenses

The Adaptive Immune Response

Microbial Pathogenesis

Microbial Diseases

Antimicrobial Chemotherapy

Clinical Microbiology and Epidemiology

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The most highly expressed proteins in a cell are the proteins and the factors.