Question 1

How do operon fusions reveal transcriptional control of a target gene?

Accepted Answer

Operon (transcriptional) fusions have a

Question 2

Describe the use of a primary and secondary antibody in a Western blot.

Accepted Answer

The primary antibody is directed against

Question 3

What variation of PCR methods can be used in directed evolution?

Accepted Answer

A)  quantitative PCR 
B)  error-prone PCR 
C)  real-time PCR 
D)  multiplex PCR 
E)  none of the above 
A)  quantitative PCR 
B)  error-prone PCR 
C)  real-time PCR 
D)  multiplex PCR 
E)  none of the above

Question 4

The migration of linear DNA molecules in agarose or polyacrylamide gels occurs at a rate that is:

Accepted Answer

A)  independent of their size 
B)  directly proportional to their size 
C)  inversely proportional to their size 
D)  dependent on the AT content 
E)  none of the above 
A)  independent of their size 
B)  directly proportional to their size 
C)  inversely proportional to their size 
D)  dependent on the AT content 
E)  none of the above

Question 5

For the reporter protein to be synthesized, the reporter gene must be fused in the proper codon reading frame with respect to:

Accepted Answer

A)  the ribosome binding site 
B)  the promoter 
C)  the vector origin of replication 
D)  the target gene 
E)  none of the above 
A)  the ribosome binding site 
B)  the promoter 
C)  the vector origin of replication 
D)  the target gene 
E)  none of the above

Question 6

What is the biochemical mechanism of acid resistance in E. coli O157:H7?

Accepted Answer

Internal pH falls when E. coli cells are

Question 7

His6-fusion-tagged proteins can be isolated by affinity chromatography because the histidine tag tightly binds to:

Accepted Answer

A)  copper 
B)  magnesium 
C)  iron 
D)  calcium 
E)  nickel 
A)  copper 
B)  magnesium 
C)  iron 
D)  calcium 
E)  nickel

Question 8

Which field of science involves the use of living organisms or their products to improve human health and the human environment?

Accepted Answer

A)  bacteriology 
B)  systematics 
C)  genomics 
D)  proteomics 
E)  biotechnology 
A)  bacteriology 
B)  systematics 
C)  genomics 
D)  proteomics 
E)  biotechnology

Question 9

The amplified product of a PCR reaction is called:

Accepted Answer

A)  replicon 
B)  operon 
C)  amplicon 
D)  cistron 
E)  none of the above 
A)  replicon 
B)  operon 
C)  amplicon 
D)  cistron 
E)  none of the above

Question 10

Describe the difference between autoradiography and phosphorimaging.

Accepted Answer

In autoradiography emissions from a radi

How do operon fusions reveal transcriptional control of a target gene?

Describe the use of a primary and secondary antibody in a Western blot.

What variation of PCR methods can be used in directed evolution?

The migration of linear DNA molecules in agarose or polyacrylamide gels occurs at a rate that is:

For the reporter protein to be synthesized, the reporter gene must be fused in the proper codon reading frame with respect to:

What is the biochemical mechanism of acid resistance in E. coli O157:H7?

His₆-fusion-tagged proteins can be isolated by affinity chromatography because the histidine tag tightly binds to:

Which field of science involves the use of living organisms or their products to improve human health and the human environment?

The amplified product of a PCR reaction is called:

Describe the difference between autoradiography and phosphorimaging.

Microbial Life: Origin and Discovery

Observing the Microbial Cell

Cell Structure and Function

Bacterial Culture, Growth, and Development

Environmental Influences and Control of Microbial Growth

Virus Structure and Function

Genomes and Chromosomes

Transcription, Translation, and Bioinformatics

Gene Transfer, Mutations, and Genome Evolution

Molecular Regulation

Viral Molecular Biology

Energetics and Catabolism

Respiration, Lithotrophy, and Photolysis

Biosynthesis

Food and Industrial Microbiology

Origins and Evolution

Bacterial Diversity

Archaeal Diversity

Eukaryotic Diversity

Microbial Ecology

Microbes and the Global Environment

Human Microbiota and Nonspecific Host Defenses

The Adaptive Immune Response

Microbial Pathogenesis

Microbial Diseases

Antimicrobial Chemotherapy

Clinical Microbiology and Epidemiology

Filters

Exam 12: Molecular Techniques and Biotechnology

How do operon fusions reveal transcriptional control of a target gene?

Describe the use of a primary and secondary antibody in a Western blot.

What variation of PCR methods can be used in directed evolution?

The migration of linear DNA molecules in agarose or polyacrylamide gels occurs at a rate that is:

For the reporter protein to be synthesized, the reporter gene must be fused in the proper codon reading frame with respect to:

What is the biochemical mechanism of acid resistance in E. coli O157:H7?

His6-fusion-tagged proteins can be isolated by affinity chromatography because the histidine tag tightly binds to:

Which field of science involves the use of living organisms or their products to improve human health and the human environment?

The amplified product of a PCR reaction is called:

Describe the difference between autoradiography and phosphorimaging.

Microbial Life: Origin and Discovery

Observing the Microbial Cell

Cell Structure and Function

Bacterial Culture, Growth, and Development

Environmental Influences and Control of Microbial Growth

Virus Structure and Function

Genomes and Chromosomes

Transcription, Translation, and Bioinformatics

Gene Transfer, Mutations, and Genome Evolution

Molecular Regulation

Viral Molecular Biology

Energetics and Catabolism

Respiration, Lithotrophy, and Photolysis

Biosynthesis

Food and Industrial Microbiology

Origins and Evolution

Bacterial Diversity

Archaeal Diversity

Eukaryotic Diversity

Microbial Ecology

Microbes and the Global Environment

Human Microbiota and Nonspecific Host Defenses

The Adaptive Immune Response

Microbial Pathogenesis

Microbial Diseases

Antimicrobial Chemotherapy

Clinical Microbiology and Epidemiology

Filters

His₆-fusion-tagged proteins can be isolated by affinity chromatography because the histidine tag tightly binds to: