Question 1

A confocal scanning optical microscope

Accepted Answer

A) uses ultraviolet light to form a specimen image. 
B) shows three-dimensional cell images from the cell surface to the middle of the cell. 
C) produces specimen images on electron micrographs. 
D) uses dyes that emit visible light when bombarded by ultraviolet rays. 
E) requires specimens to be stained. 
A) uses ultraviolet light to form a specimen image. 
B) shows three-dimensional cell images from the cell surface to the middle of the cell. 
C) produces specimen images on electron micrographs. 
D) uses dyes that emit visible light when bombarded by ultraviolet rays. 
E) requires specimens to be stained.

Question 2

Which of the following controls the amount of light entering the condenser?

Accepted Answer

A) objective lens 
B) ocular lens 
C) condenser 
D) body 
E) iris diaphragm 
A) objective lens 
B) ocular lens 
C) condenser 
D) body 
E) iris diaphragm

Question 3

What is the term for a culture made from one isolated colony?

Accepted Answer

A) axenic 
B) mixed 
C) pure 
D) Both axenic and mixed are correct. 
E) Both axenic and pure are correct. 
A) axenic 
B) mixed 
C) pure 
D) Both axenic and mixed are correct. 
E) Both axenic and pure are correct.

Question 4

A nutrient medium that has all of its chemical components identified and their precise concentrations known and reproducible,is termed

Accepted Answer

A) complex. 
B) reducing. 
C) enriched. 
D) synthetic. 
A) complex. 
B) reducing. 
C) enriched. 
D) synthetic.

Question 5

The Five I's of studying microorganisms include all of the following except

Accepted Answer

A) inoculation. 
B) incubation. 
C) infection. 
D) isolation. 
E) identification. 
A) inoculation. 
B) incubation. 
C) infection. 
D) isolation. 
E) identification.

Question 6

Some microbes are not capable of growing on artificial media.

Accepted Answer

A) True 
 B)False

Question 7

Differential media result in which of the following growth characteristics?

Accepted Answer

A) different color colonies 
B) different media color post incubation 
C) precipitates 
D) gas bubbles 
E) All of the choices are correct. 
A) different color colonies 
B) different media color post incubation 
C) precipitates 
D) gas bubbles 
E) All of the choices are correct.

Question 8

The purpose of staining cells on a microscope slide is to

Accepted Answer

A) kill them. 
B) secure them to the slide. 
C) enlarge the cells. 
D) add contrast in order to see them better. 
E) see motility. 
A) kill them. 
B) secure them to the slide. 
C) enlarge the cells. 
D) add contrast in order to see them better. 
E) see motility.

Question 9

Eosin-methylene blue agar,or EMB,is a commonly used bacteriological medium in a lab,used to grow gram-negative bacteria.There are two dyes in it,eosin and methylene blue,as well as the sugar lactose.Eosin is a pH indicator that changes color when the medium is acidic,while methylene blue reduces the growth of most gram-positive bacteria.If an organism uses lactose,acid will be produced changing the pH of the medium and subsequently causing the bacterial colonies to change color.
Based upon this description,this medium is:

Accepted Answer

A) reducing 
B) enrichment 
C) selective 
D) differential 
E) differential and selective 
A) reducing 
B) enrichment 
C) selective 
D) differential 
E) differential and selective

Question 10

Bacterial cultures are easily identified from their microscopic appearance.

Accepted Answer

A) True 
 B)False

Question 11

Which type of microscope cannot image live specimens?

Accepted Answer

A) bright-field 
B) phase-contrast 
C) dark-field 
D) electron 
E) differential interference 
A) bright-field 
B) phase-contrast 
C) dark-field 
D) electron 
E) differential interference

Question 12

Let us say that the 100X magnification lens of your microscope has a resolving power of 0.15 microns (micrometers).The specimen that you will be looking at has bacterial cocci the size of 0.1 microns in diameter.Which statement is correct?

Accepted Answer

A) The cocci will be easily seen and very clear. 
B) The cocci will be fuzzy-looking and blend together. 
C) The cocci will appear much smaller than they really are because of the small resolving power. 
D) The cocci will be totally invisible. 
A) The cocci will be easily seen and very clear. 
B) The cocci will be fuzzy-looking and blend together. 
C) The cocci will appear much smaller than they really are because of the small resolving power. 
D) The cocci will be totally invisible.

Question 13

Magnification is achieved in a compound microscope through the initial magnification of the specimen by the _____ lens.This image is then projected to the _____ lens that will further magnify the specimen to form a virtual image received by the eye.

Accepted Answer

A) ocular;objective 
B) scanning;objective 
C) objective;ocular 
D) ocular;oil immersion 
A) ocular;objective 
B) scanning;objective 
C) objective;ocular 
D) ocular;oil immersion

Question 14

A medium that is gel-like has less agar in it compared to a solid medium.

Accepted Answer

A) True 
 B)False

Question 15

A reducing medium is used to culture

Accepted Answer

A) fastidious organisms. 
B) aerobic organisms. 
C) anaerobic organisms. 
D) any pathogenic organisms. 
A) fastidious organisms. 
B) aerobic organisms. 
C) anaerobic organisms. 
D) any pathogenic organisms.

Question 16

One colony typically develops from the growth of several parent bacterial cells.

Accepted Answer

A) True 
 B)False

Question 17

A microbiologist must culture a patient's feces for intestinal pathogens.Which of the following would likely be present in selective media for analyzing this fecal specimen?

Accepted Answer

A) NaCl 
B) sheep red blood cells 
C) bile salts 
D) thioglycollic acid 
E) peptone 
A) NaCl 
B) sheep red blood cells 
C) bile salts 
D) thioglycollic acid 
E) peptone

Question 18

Which type of medium is able to distinguish different species or types of microorganisms based on an observable change in the colonies or in the medium?

Accepted Answer

A) differential 
B) selective 
C) enumeration 
D) enriched 
E) reducing 
A) differential 
B) selective 
C) enumeration 
D) enriched 
E) reducing

Question 19

A pure culture contains

Accepted Answer

A) only one identified species of microorganism. 
B) only bacteria. 
C) a variety of microbes from one source. 
D) a variety of species from the same genus. 
A) only one identified species of microorganism. 
B) only bacteria. 
C) a variety of microbes from one source. 
D) a variety of species from the same genus.

Question 20

The _____ of the microscope holds and allows selection of the objective lenses.

Accepted Answer

A) stage 
B) condenser 
C) objective 
D) ocular 
E) nosepiece 
A) stage 
B) condenser 
C) objective 
D) ocular 
E) nosepiece

Exam 3: Tools of the Laboratory: Methods for the Culturing of Microscopic Analysis of Microorganisms

A confocal scanning optical microscope

Which of the following controls the amount of light entering the condenser?

What is the term for a culture made from one isolated colony?

A nutrient medium that has all of its chemical components identified and their precise concentrations known and reproducible,is termed

The Five I's of studying microorganisms include all of the following except

Some microbes are not capable of growing on artificial media.

Differential media result in which of the following growth characteristics?

The purpose of staining cells on a microscope slide is to

Bacterial cultures are easily identified from their microscopic appearance.

Which type of microscope cannot image live specimens?

Let us say that the 100X magnification lens of your microscope has a resolving power of 0.15 microns (micrometers).The specimen that you will be looking at has bacterial cocci the size of 0.1 microns in diameter.Which statement is correct?

Magnification is achieved in a compound microscope through the initial magnification of the specimen by the _____ lens.This image is then projected to the _____ lens that will further magnify the specimen to form a virtual image received by the eye.

A medium that is gel-like has less agar in it compared to a solid medium.

A reducing medium is used to culture

One colony typically develops from the growth of several parent bacterial cells.

A microbiologist must culture a patient's feces for intestinal pathogens.Which of the following would likely be present in selective media for analyzing this fecal specimen?

Which type of medium is able to distinguish different species or types of microorganisms based on an observable change in the colonies or in the medium?

A pure culture contains

The _____ of the microscope holds and allows selection of the objective lenses.

The Main Themes of Microbiology

The Chemistry of Biology

Prokaryotic Profiles: The Bacteria and Archaea

Eukaryotic Cells and Microorganisms

An Introduction to the Viruses

Microbial Nutrition, Ecology, and Growth

Microbial Metabolism: the Chemical Crossroads of Life

Microbial Genetics

Genetic Engineering and Recombinant Dna

Physical and Chemical Control of Microbes

Drugs, Microbes, host-The Elements of Chemotherapy

Microbe-Human Interactions: Infection and Disease

Host Defenses I: Overview and Nonspecific Defenses

Host Defenses II: Specific Immunity and Immunization

Disorders in Immunity

Diagnosing Infections

Infectious Diseases Affecting the Skin and Eyes

Infectious Diseases Affecting the Nervous System

Infectious Diseases Affecting the Cardiovascular and Lymphatic Systems

Infectious Diseases Affecting the Respiratory System

Infectious Diseases Affecting the Gastrointestinal Tract

Infectious Diseases Affecting the Genitourinary System

Microbes and the Environment

Applied Microbiology and Food and Water Safety

Filters

Magnification is achieved in a compound microscope through the initial magnification of the specimen by the _ lens.This image is then projected to the _ lens that will further magnify the specimen to form a virtual image received by the eye.