Question 1

Newly inoculated cultures must be _____ at a specific temperature and time to encourage growth.

Accepted Answer

A) streaked 
B) poured 
C) incubated 
D) spread 
A) streaked 
B) poured 
C) incubated 
D) spread

Question 2

Which type of microscope shows cells against a bright background and also shows intracellular structures of unstained cells based on their varying densities?

Accepted Answer

A) bright-field 
B) dark-field 
C) phase-contrast 
D) differential interference 
E) electron 
A) bright-field 
B) dark-field 
C) phase-contrast 
D) differential interference 
E) electron

Question 3

A nutrient medium that contains at least one ingredient that is not chemically definable is termed

Accepted Answer

A) complex. 
B) reducing. 
C) enriched. 
D) synthetic. 
A) complex. 
B) reducing. 
C) enriched. 
D) synthetic.

Question 4

Robert Koch and his lab workers first used agar in bacteriological media.Why did they find this media so much better than previous materials used to grow bacteria?

Accepted Answer

A) Bacteria,growing on the solidified media,did not degrade or break down the medium. 
B) Agar could convert from liquid to solid form,and back again,very easily. 
C) The food material that Koch was previously using in the lab was much more expensive than agar. 
D) Agar is a great source of nutrients for bacteria. 
A) Bacteria,growing on the solidified media,did not degrade or break down the medium. 
B) Agar could convert from liquid to solid form,and back again,very easily. 
C) The food material that Koch was previously using in the lab was much more expensive than agar. 
D) Agar is a great source of nutrients for bacteria.

Question 5

Who invented the first crude microscope by grinding glass?

Accepted Answer

A) Redi 
B) Lister 
C) Schultz and Schwann 
D) Leeuwenhoek 
E) Pasteur 
A) Redi 
B) Lister 
C) Schultz and Schwann 
D) Leeuwenhoek 
E) Pasteur

Question 6

A microbiologist decides to use a nutrient medium that contains thioglycollic acid.What type of microbe is she attempting to culture?

Accepted Answer

A) fastidious 
B) gram-positive 
C) anaerobic 
D) gram-negative 
E) aerobic 
A) fastidious 
B) gram-positive 
C) anaerobic 
D) gram-negative 
E) aerobic

Question 7

A reducing medium contains

Accepted Answer

A) sugars that can be fermented. 
B) extra oxygen. 
C) hemoglobin,vitamins,or other growth factors. 
D) substances that remove oxygen. 
E) inhibiting agents. 
A) sugars that can be fermented. 
B) extra oxygen. 
C) hemoglobin,vitamins,or other growth factors. 
D) substances that remove oxygen. 
E) inhibiting agents.

Question 8

The procedures for culturing a microorganism require the use of a microscope.

Accepted Answer

A) True 
 B)False

Question 9

A microbiologist inoculates Staphylococcus aureus into a culture medium.Following incubation,both Staphylococcus aureus and Staphylococcus epidermidis are determined to be growing in this culture.What is the most likely explanation?

Accepted Answer

A) The microbiologist used too much inoculum. 
B) The culture is contaminated. 
C) The incubation temperature was incorrect. 
D) The culture medium must be selective. 
E) The culture medium must be differential. 
A) The microbiologist used too much inoculum. 
B) The culture is contaminated. 
C) The incubation temperature was incorrect. 
D) The culture medium must be selective. 
E) The culture medium must be differential.

Question 10

At the end of the Gram stain,gram-positive bacteria will be seen as pink cells.

Accepted Answer

A) True 
 B)False

Question 11

Fixed smears of specimens are required in order to perform the Gram stain and endospore stain on the specimens.

Accepted Answer

A) True 
 B)False

Question 12

Basic dyes are

Accepted Answer

A) attracted to the acidic substances of bacterial cells. 
B) anionic. 
C) used in negative staining. 
D) repelled by cells. 
E) dyes such as India ink and nigrosin. 
A) attracted to the acidic substances of bacterial cells. 
B) anionic. 
C) used in negative staining. 
D) repelled by cells. 
E) dyes such as India ink and nigrosin.

Question 13

The term that refers to the purposeful addition of microorganisms into a laboratory nutrient medium is

Accepted Answer

A) isolation. 
B) inoculation. 
C) immunization. 
D) infection. 
E) contamination. 
A) isolation. 
B) inoculation. 
C) immunization. 
D) infection. 
E) contamination.

Question 14

Agar is a complex polysaccharide that comes from a/an

Accepted Answer

A) green plant. 
B) fungus. 
C) mold. 
D) algae. 
E) Euglena. 
A) green plant. 
B) fungus. 
C) mold. 
D) algae. 
E) Euglena.

Question 15

A common medium used for growing fastidious bacteria is

Accepted Answer

A) blood agar. 
B) trypticase soy agar. 
C) mannitol salt agar. 
D) MacConkey medium. 
E) reducing medium. 
A) blood agar. 
B) trypticase soy agar. 
C) mannitol salt agar. 
D) MacConkey medium. 
E) reducing medium.

Question 16

The specimen preparation that is best for viewing cell motility is

Accepted Answer

A) hanging drop. 
B) fixed,stained smear. 
C) Gram stain. 
D) negative stain. 
E) flagellar stain. 
A) hanging drop. 
B) fixed,stained smear. 
C) Gram stain. 
D) negative stain. 
E) flagellar stain.

Question 17

Which of the following magnifies the specimen to produce the virtual image of the specimen?

Accepted Answer

A) objective lens 
B) ocular lens 
C) condenser 
D) body 
E) iris diaphragm 
A) objective lens 
B) ocular lens 
C) condenser 
D) body 
E) iris diaphragm

Question 18

The type of microscope in which you would see brightly illuminated specimens against a black background is

Accepted Answer

A) bright-field. 
B) dark-field. 
C) phase-contrast. 
D) fluorescence. 
E) electron. 
A) bright-field. 
B) dark-field. 
C) phase-contrast. 
D) fluorescence. 
E) electron.

Question 19

Which type of microscope does not use light in forming the specimen image?

Accepted Answer

A) bright-field 
B) dark-field 
C) phase-contrast 
D) fluorescence 
E) electron 
A) bright-field 
B) dark-field 
C) phase-contrast 
D) fluorescence 
E) electron

Question 20

_____ has the same optical qualities as glass and thus prevents refractive loss of light as it passes from the slide to the objective lens.

Accepted Answer

A) The scanning objective 
B) The oil objective 
C) The slide 
D) Immersion oil 
E) The ocular 
A) The scanning objective 
B) The oil objective 
C) The slide 
D) Immersion oil 
E) The ocular

Exam 3: Tools of the Laboratory: Methods for the Culturing of Microscopic Analysis of Microorganisms

Newly inoculated cultures must be _____ at a specific temperature and time to encourage growth.

Which type of microscope shows cells against a bright background and also shows intracellular structures of unstained cells based on their varying densities?

A nutrient medium that contains at least one ingredient that is not chemically definable is termed

Robert Koch and his lab workers first used agar in bacteriological media.Why did they find this media so much better than previous materials used to grow bacteria?

Who invented the first crude microscope by grinding glass?

A microbiologist decides to use a nutrient medium that contains thioglycollic acid.What type of microbe is she attempting to culture?

A reducing medium contains

The procedures for culturing a microorganism require the use of a microscope.

A microbiologist inoculates Staphylococcus aureus into a culture medium.Following incubation,both Staphylococcus aureus and Staphylococcus epidermidis are determined to be growing in this culture.What is the most likely explanation?

At the end of the Gram stain,gram-positive bacteria will be seen as pink cells.

Fixed smears of specimens are required in order to perform the Gram stain and endospore stain on the specimens.

Basic dyes are

The term that refers to the purposeful addition of microorganisms into a laboratory nutrient medium is

Agar is a complex polysaccharide that comes from a/an

A common medium used for growing fastidious bacteria is

The specimen preparation that is best for viewing cell motility is

Which of the following magnifies the specimen to produce the virtual image of the specimen?

The type of microscope in which you would see brightly illuminated specimens against a black background is

Which type of microscope does not use light in forming the specimen image?

_____ has the same optical qualities as glass and thus prevents refractive loss of light as it passes from the slide to the objective lens.

The Main Themes of Microbiology

The Chemistry of Biology

Prokaryotic Profiles: The Bacteria and Archaea

Eukaryotic Cells and Microorganisms

An Introduction to the Viruses

Microbial Nutrition, Ecology, and Growth

Microbial Metabolism: the Chemical Crossroads of Life

Microbial Genetics

Genetic Engineering and Recombinant Dna

Physical and Chemical Control of Microbes

Drugs, Microbes, host-The Elements of Chemotherapy

Microbe-Human Interactions: Infection and Disease

Host Defenses I: Overview and Nonspecific Defenses

Host Defenses II: Specific Immunity and Immunization

Disorders in Immunity

Diagnosing Infections

Infectious Diseases Affecting the Skin and Eyes

Infectious Diseases Affecting the Nervous System

Infectious Diseases Affecting the Cardiovascular and Lymphatic Systems

Infectious Diseases Affecting the Respiratory System

Infectious Diseases Affecting the Gastrointestinal Tract

Infectious Diseases Affecting the Genitourinary System

Microbes and the Environment

Applied Microbiology and Food and Water Safety

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