Question 1

What is the relative centrifugal force (RCF)for a centrifuge spinning at 1200 rpm with a rotor head radius of 8 inches?

Accepted Answer

A)  32.7 g 
B)  3200 g 
C)  130 g 
D)  1300 g 
A)  32.7 g 
B)  3200 g 
C)  130 g 
D)  1300 g A

Question 2

The National Institute for Occupational Safety and Health of the CDC distributes a document that specifies how U.S.clinical laboratories should handle infectious agents.This document is referred to as:

Accepted Answer

A)  the Chemical Hygiene Plan. 
B)  the Exposure Control Plan. 
C)  HAZMAT. 
D)  Universal Precautions. 
A)  the Chemical Hygiene Plan. 
B)  the Exposure Control Plan. 
C)  HAZMAT. 
D)  Universal Precautions. D

Question 3

You have seen another laboratorian collecting blood from a patient without using any barrier protection.What safety mandate is this employee ignoring?

Accepted Answer

A)  Chemical hygiene plan 
B)  Universal Precautions 
C)  Clinical laboratory plan 
D)  Tuberculosis exposure plan 
A)  Chemical hygiene plan 
B)  Universal Precautions 
C)  Clinical laboratory plan 
D)  Tuberculosis exposure plan B

Question 4

What is the Clinical and Laboratory Standards Institute (CLSI)recommendation for the most appropriate RCF and time for centrifugation of blood samples to obtain sufficient separation of cellular and liquid components?

Accepted Answer

A)  2000 g for 20 min 
B)  1200 g for 10 min 
C)  500 g for 10 min 
D)  100 g for 15 min 
A)  2000 g for 20 min 
B)  1200 g for 10 min 
C)  500 g for 10 min 
D)  100 g for 15 min

Question 5

How many grams of NaCl (MW = 58.5 g)are required to prepare 250 mL of a 6 mol/L solution of NaCl?

Accepted Answer

A)  87.75 g 
B)  58.5 g 
C)  585 g 
D)  877.5 g 
A)  87.75 g 
B)  58.5 g 
C)  585 g 
D)  877.5 g

Question 6

To make 20 mL of a 0.1 g/L solution from a 10 mg/mL stock solution,you should add _____ mL of the stock solution to _____ mL of solvent.

Accepted Answer

A)  2; 18 
B)  10; 10 
C)  1; 19 
D)  0.2; 19.8 
A)  2; 18 
B)  10; 10 
C)  1; 19 
D)  0.2; 19.8

Question 7

Reverse osmosis is:

Accepted Answer

A)  the process of vaporizing and condensing a liquid to purify or concentrate a substance or to separate a volatile substance from less volatile substances. 
B)  a process by which water is forced through a semipermeable membrane that acts as a molecular filter. 
C)  a process that removes ions to produce mineral-free deionized water. 
D)  a process used to convert a liquid or a volatile solid into vapor. 
A)  the process of vaporizing and condensing a liquid to purify or concentrate a substance or to separate a volatile substance from less volatile substances. 
B)  a process by which water is forced through a semipermeable membrane that acts as a molecular filter. 
C)  a process that removes ions to produce mineral-free deionized water. 
D)  a process used to convert a liquid or a volatile solid into vapor.

Question 8

The class of fire that involves flammable liquids and gases is class and the recommended extinguisher agent is:

Accepted Answer

A)  A; water. 
B)  B; water. 
C)  B; dry chemical or carbon dioxide. 
D)  C; Halon 1211 foam. 
A)  A; water. 
B)  B; water. 
C)  B; dry chemical or carbon dioxide. 
D)  C; Halon 1211 foam.

Question 9

Which one of the following statements concerning type I water is not correct?

Accepted Answer

A)  Type I water is acceptable for analytical purposes. 
B)  Testing that requires minimal interferences (such as iron or enzyme analysis)requires the use of type I water. 
C)  Type I water is obtained from distillation only and contains a few impurities. 
D)  Type I water results in part from ion exchange purification and reverse osmosis. 
A)  Type I water is acceptable for analytical purposes. 
B)  Testing that requires minimal interferences (such as iron or enzyme analysis)requires the use of type I water. 
C)  Type I water is obtained from distillation only and contains a few impurities. 
D)  Type I water results in part from ion exchange purification and reverse osmosis.

Question 10

The maximum amount of working volume of a flammable solvent allowed outside a storage cabinet is 1 gallon per room.

Accepted Answer

A) True 
 B)False

Question 11

Chemicals with the designation _____ are suitable for use in most clinical chemistry laboratory analyses.

Accepted Answer

A)  technical grade 
B)  analytical reagent grade 
C)  USP (United States Pharmacopeia) 
D)  NF (National Formulary) 
A)  technical grade 
B)  analytical reagent grade 
C)  USP (United States Pharmacopeia) 
D)  NF (National Formulary)

Question 12

Universal Precautions:

Accepted Answer

A)  consist of shipping standards that divide potentially infectious specimens or substances into risk groups. 
B)  state that only HIV,hepatitis,and sexually transmitted disease-positive specimens should be considered and handled as infectious material. 
C)  state that hand washing is only necessary before entering a clinical laboratory. 
D)  consist of guidelines that in part necessitate the wearing of barrier protection when handling potentially infectious material. 
A)  consist of shipping standards that divide potentially infectious specimens or substances into risk groups. 
B)  state that only HIV,hepatitis,and sexually transmitted disease-positive specimens should be considered and handled as infectious material. 
C)  state that hand washing is only necessary before entering a clinical laboratory. 
D)  consist of guidelines that in part necessitate the wearing of barrier protection when handling potentially infectious material.

Question 13

What is the normality of a solution containing 20 g of H2SO4 (MW = 98)in 500 mL of buffer?

Accepted Answer

A)  8.2 N 
B)  2.8 N 
C)  0.82 N 
D)  176.8 N 
A)  8.2 N 
B)  2.8 N 
C)  0.82 N 
D)  176.8 N

Question 14

The increased use of electronic health record (EHR)systems has amplified the need for standardized communication between EHR and other systems.The newest universal code system that has standardized terms for all kinds of laboratory observations and measurements and includes a code number,a long formal name,a shorter name,and synonyms is referred to as the:

Accepted Answer

A)  Occupational Safety and Health Act. 
B)  Clinical and Laboratory Standards Institute system. 
C)  Logical Observation Identifier Names and Codes system. 
D)  National Fire Protection Association system. 
A)  Occupational Safety and Health Act. 
B)  Clinical and Laboratory Standards Institute system. 
C)  Logical Observation Identifier Names and Codes system. 
D)  National Fire Protection Association system.

Question 15

Regarding centrifugation,what is the formula for calculating relative centrifugal force (RCF)?

Accepted Answer

A)  RCF = 1.118 * r (in cm) * rpm2 
B)  RCF = 1.118 * 10-5 * r (in cm) * rpm2 
C)  RCF = 1.118 * 10-5 * r2 (in cm) * rpm 
D)  RCF = 1.118 * r (in inches)* rpm2 
A)  RCF = 1.118 * r (in cm) * rpm2 
B)  RCF = 1.118 * 10-5 * r (in cm) * rpm2 
C)  RCF = 1.118 * 10-5 * r2 (in cm) * rpm 
D)  RCF = 1.118 * r (in inches)* rpm2

Question 16

The one technical aspect of a clinical laboratory that has received considerable attention in the need for ergonomic design to decrease musculoskeletal stress would be:

Accepted Answer

A)  pipetting. 
B)  chair design. 
C)  lighting. 
D)  flooring. 
A)  pipetting. 
B)  chair design. 
C)  lighting. 
D)  flooring.

Question 17

A highly purified chemical that is directly weighed or measured to produce a solution whose concentration is exactly known is referred to as a:

Accepted Answer

A)  calibrator. 
B)  primary reference material. 
C)  secondary standard. 
D)  working solution. 
A)  calibrator. 
B)  primary reference material. 
C)  secondary standard. 
D)  working solution.

Question 18

Fifty milligrams (mg)per 1000 mL is equal to:

Accepted Answer

A)  0.005 g/mL. 
B)  0.050 g/mL. 
C)  0.050 g/L. 
D)  0.50 mg/mL. 
A)  0.005 g/mL. 
B)  0.050 g/mL. 
C)  0.050 g/L. 
D)  0.50 mg/mL.

Question 19

The type of pipette that is a piece of glass tubing drawn out to a tip and graduated uniformly along its length and is used for reagent measurement is referred to as a:

Accepted Answer

A)  transfer pipette. 
B)  volumetric pipette. 
C)  micropipette. 
D)  measuring pipette. 
A)  transfer pipette. 
B)  volumetric pipette. 
C)  micropipette. 
D)  measuring pipette.

Question 20

What is the molarity (mol/L)of a solution containing 25 g of H2SO4 (MW= 98 g)in 150 mL of deionized water?

Accepted Answer

A)  1.7 mol/L 
B)  0.59 mol/L 
C)  0.002 mol/L 
D)  16.3 mol/L 
A)  1.7 mol/L 
B)  0.59 mol/L 
C)  0.002 mol/L 
D)  16.3 mol/L

Exam 8: Principles of Basic Techniques and Laboratory Safety

What is the relative centrifugal force (RCF)for a centrifuge spinning at 1200 rpm with a rotor head radius of 8 inches?

The National Institute for Occupational Safety and Health of the CDC distributes a document that specifies how U.S.clinical laboratories should handle infectious agents.This document is referred to as:

You have seen another laboratorian collecting blood from a patient without using any barrier protection.What safety mandate is this employee ignoring?

What is the Clinical and Laboratory Standards Institute (CLSI)recommendation for the most appropriate RCF and time for centrifugation of blood samples to obtain sufficient separation of cellular and liquid components?

How many grams of NaCl (MW = 58.5 g)are required to prepare 250 mL of a 6 mol/L solution of NaCl?

To make 20 mL of a 0.1 g/L solution from a 10 mg/mL stock solution,you should add _____ mL of the stock solution to _____ mL of solvent.

Reverse osmosis is:

The class of fire that involves flammable liquids and gases is class and the recommended extinguisher agent is:

Which one of the following statements concerning type I water is not correct?

The maximum amount of working volume of a flammable solvent allowed outside a storage cabinet is 1 gallon per room.

Chemicals with the designation _____ are suitable for use in most clinical chemistry laboratory analyses.

Universal Precautions:

What is the normality of a solution containing 20 g of H2SO4 (MW = 98)in 500 mL of buffer?

Regarding centrifugation,what is the formula for calculating relative centrifugal force (RCF)?

The one technical aspect of a clinical laboratory that has received considerable attention in the need for ergonomic design to decrease musculoskeletal stress would be:

A highly purified chemical that is directly weighed or measured to produce a solution whose concentration is exactly known is referred to as a:

Fifty milligrams (mg)per 1000 mL is equal to:

The type of pipette that is a piece of glass tubing drawn out to a tip and graduated uniformly along its length and is used for reagent measurement is referred to as a:

What is the molarity (mol/L)of a solution containing 25 g of H2SO4 (MW= 98 g)in 150 mL of deionized water?

Clinical Chemistry, molecular Diagnostics, and Laboratory Medicine

Selection and Analytical Evaluation of Methodswith Statistical Techniques

Clinical Evaluation of Methods

Evidence-Based Laboratory Medicine

Establishment and Use of Reference Values

Specimen Collection, processing, and Other Preanalytical Variables

Quality Management

Optical Techniques

Electrochemistry and Chemical Sensors

Electrophoresis

Chromatography

Mass Spectrometry

Enzyme and Rate Analyses

Immunochemical Techniques

Automation

Point-Of-Care Instrumentation

Amino Acids, peptides, and Proteins

Serum Enzymes

Tumor Markers and Cancer Genes

Kidney Function Test-Screatinine, Urea, and Uric Acid

Carbohydrates

Lipids, lip-Oproteins, apolipoproteins, and Other Cardiac Risk Factors

Electrolytes and Blood Gases

Hormones

Catecholamines and Serotonin

Vitamins, Trace Elements, and Nutritional Assessment

Hemoglobin, Iron, and Bilirubin

Porphyrins and Porphyrias

Therapeutic Drugs and Their Management

Clinical Toxicology

Toxic Metals

Diabetes

Cardiovascular Disease

Kidney Disease

Physiology and Disorders of Water,Electrolyte,and Acid-Base Metabolism

Liver Disease

Gastrointestinal and Pancreatic Diseases

Disorders of Bone and Mineral Metabolism

Disorders of the Pituitary

Disorders of the Adrenal Cortex

Thyroid Disorders

Reproduction-Related Disorders

Pregnancy and Prenatal Testing

Newborn Screening and Inborn Errors of Metabolism

Pharmacogenetics

Principles of Molecular Biology

Nucleic Acid Techniques and Applications

Genomes and Nucleic Acid Alterations

Filters

To make 20 mL of a 0.1 g/L solution from a 10 mg/mL stock solution,you should add _ mL of the stock solution to _ mL of solvent.

What is the normality of a solution containing 20 g of H₂SO₄ (MW = 98)in 500 mL of buffer?

What is the molarity (mol/L)of a solution containing 25 g of H₂SO₄ (MW= 98 g)in 150 mL of deionized water?