Exam 14: Enzyme and Rate Analyses

The interaction of the amino acid side chains with the arrangement of the $\alpha$ -helices and $\beta$ -sheets to form a three-dimensional protein structure is called the _____ structure of the protein.

An example of a posttranslational modification of an enzyme that produces an enzyme isoform would be:

The reciprocal plot pictured below indicates a decrease in V_max and a decrease in K_m.The solid line indicates a normal enzymatic reaction.What type of enzymatic inhibition is this?

Varying different factors and studying their effects on an enzymatic reaction rate in the assessment of most favorable reaction conditions for an enzyme assay is referred to as:

In a continuous-monitoring assessment of an enzyme reaction rate,which one of the following is the preferable measurement?

In regard to the expression of enzyme activity,a katal is:

Zero-order kinetics occurs during the beginning of an enzyme-catalyzed reaction when a substrate concentration is high and the rate of the reaction is _____ on the _____ concentration.

Activators increase the rates of enzyme-catalyzed reactions.In some cases,these activators interact with the nonenzymatic component of the reaction such as the substrate.However,in most cases the activator:

Immobilized enzymes are used analytically in various electrochemical techniques.The use of an ion-selective electrode that is coated with an enzyme that produces ions when placed in a substrate solution is a type of:

How does pH alter an enzymatic reaction rate?

Which one of the following is a correct statement describing a property of an enzyme?

In the reciprocal plot pictured below,the solid line indicates a normal enzyme reaction with no inhibition,and the dotted line indicates a decrease in V_max and no change in K_m.This plot is an example of which type of inhibition?

All enzymes are classified to one of six classes based on the reaction they catalyze.Based on this classification,creatine kinase is a member of which one of the following enzyme classes?

During embryonic and fetal development,changes in isoenzyme distribution patterns are common.These changes are thought to result from:

Regarding enzyme kinetics,the substrate concentration at which the reaction velocity is equal to 0.5 V_max is referred to as:

When determining the activity of an enzyme in serum as in a bisubstrate reaction,measurement of two different substances can be made.One measurement determines the decrease in substrate concentration acted upon by the enzyme and the other:

Regarding enzyme kinetics,the Michaelis-Menten plot of the relationship between reaction velocity and substrate concentration is correctly expressed as which one of the following formulae?

In regard to factors that govern the rate of an enzymatic reaction,first-order reaction kinetics occur at that part of the reaction during which the rate of the reaction is:

True isoenzymes,which are multiple forms of an enzyme that possesses the ability to catalyze an enzyme's characteristic reaction,are formed by:

In an enzyme immunoassay such as ELISA,the specificity of the labeled enzyme is the most important aspect of the measurement.