Question 1

Which technique used to diagnose a disease is also called the double diffusion method?

Accepted Answer

A) Ouchterlony test 
B) VDRL test 
C) immunoelectrophoresis 
D) Western blot 
E) ELISA 
A) Ouchterlony test 
B) VDRL test 
C) immunoelectrophoresis 
D) Western blot 
E) ELISA

Question 2

Broad general characteristics of bacteria on flowcharts can be misleading for a diagnosis of disease.

Accepted Answer

A) True 
 B)False

Question 3

The Widal test is used for diagnosing salmonellosis.

Accepted Answer

A) True 
 B)False

Question 4

Mary had an accidental needlestick one night during her shift as an emergency medical technician.Initially, she tested positive for HIV by the ELISA test.Which test would be done next to confirm an HIV infection?

Accepted Answer

A) immunoelectrophoresis 
B) Ouchterlony 
C) complement fixation 
D) Western blot 
E) radioimmunoassay 
A) immunoelectrophoresis 
B) Ouchterlony 
C) complement fixation 
D) Western blot 
E) radioimmunoassay

Question 5

A simple method to detect _____ cells involves mixing them with untreated sheep red blood cells that will bind and form rosettes.

Accepted Answer

A) True 
 B)False

Question 6

Serological tests should have low sensitivity and specificity.

Accepted Answer

A) True 
 B)False

Question 7

Which test is used to measure the IgE present in allergic patients?

Accepted Answer

A) fluorescent antibody tests 
B) Rapid Plasma Reagin (RPR) test 
C) Weil-Felix reaction 
D) RIST 
E) ELISA 
A) fluorescent antibody tests 
B) Rapid Plasma Reagin (RPR) test 
C) Weil-Felix reaction 
D) RIST 
E) ELISA

Question 8

Identify, describe, and discuss the objectives of the three main categories of identifying infectious agents using specific examples.

Accepted Answer

The three main categories of identifying

Question 9

When antibodies or other substances in serum cross-react with the test reagents, a false positive result can occur.

Accepted Answer

A) True 
 B)False

Question 10

Affixing antigen to an inert particle such as a latex bead is sometimes used in which type of test?

Accepted Answer

A) cross-reactions 
B) agglutination 
C) precipitation 
D) specificity 
E) sensitivity 
A) cross-reactions 
B) agglutination 
C) precipitation 
D) specificity 
E) sensitivity

Question 11

In lab, a student observes some dull, wrinkled brown colonies growing on their agar.They gram stain the sample and observe single bacilli.Their blood agar does not reveal any hemolysis while their MSA has growth and fermentation.Using the following table and the given observations, determine which microorganism this would most likely be.

Accepted Answer

A) E. coli 
B) Pseudomonas aeruginosa 
C) B. subtilis 
D) B. cereus 
A) E. coli 
B) Pseudomonas aeruginosa 
C) B. subtilis 
D) B. cereus

Question 12

Serological testing always involves reactions between specific antibody and antigen.

Accepted Answer

A) True 
 B)False

Question 13

Soluble antigens are detected in which type of test?

Accepted Answer

A) cross-reactions 
B) agglutination 
C) precipitation 
D) specificity 
E) sensitivity 
A) cross-reactions 
B) agglutination 
C) precipitation 
D) specificity 
E) sensitivity

Question 14

Why do many physicians feel that stool cultures are not necessary, except in certain circumstances when diagnosing GI tract infections?

Accepted Answer

Many physicians feel that stool cultures

Question 15

In direct antigen testing, the reaction is generally seen with the:

Accepted Answer

A) electron microscope. 
B) naked eye. 
C) light microscope. 
D) API 20E miniature identification system. 
E) PCR reaction. 
A) electron microscope. 
B) naked eye. 
C) light microscope. 
D) API 20E miniature identification system. 
E) PCR reaction.

Question 16

When collecting __________, a "clean catch" method is often used.

Accepted Answer

The answer of When collecting __________, a "clean catch" method...

Question 17

The indirect ELISA test detects ______ in a patient's blood.

Accepted Answer

A) antigen 
B) antibody 
C) microorganisms 
D) IgE only 
E) complement 
A) antigen 
B) antibody 
C) microorganisms 
D) IgE only 
E) complement

Question 18

Serotyping of the pneumococcus based on capsular polysaccharide is performed in:

Accepted Answer

A) Ouchterlony double diffusion. 
B) Western blot. 
C) immunelectrophoresis. 
D) radioimmunoassay (RI
A) . 
E) the Quellung test. 
A) Ouchterlony double diffusion. 
B) Western blot. 
C) immunelectrophoresis. 
D) radioimmunoassay (RI
A) . 
E) the Quellung test.

Question 19

The more a sample can be diluted and yet still react with antigen, the ______ the concentration of antibodies in that sample and the _______ is its titer.

Accepted Answer

A) lower, lower 
B) higher, lower 
C) lower, higher 
D) higher, higher 
A) lower, lower 
B) higher, lower 
C) lower, higher 
D) higher, higher

Question 20

Which of the following choices are true about viral hemagglutination testing?

Accepted Answer

A) It uses a red blood cell that naturally reacts with viral antigens. 
B) It analyzes patient serum for specific antibodies to a virus. 
C) It has hemagglutination if the patient serum lacks virus specific antibodies. 
D) it is used to diagnosis viral diseases such as rubella and mononucleosis. 
E) All of the choices are true. 
A) It uses a red blood cell that naturally reacts with viral antigens. 
B) It analyzes patient serum for specific antibodies to a virus. 
C) It has hemagglutination if the patient serum lacks virus specific antibodies. 
D) it is used to diagnosis viral diseases such as rubella and mononucleosis. 
E) All of the choices are true.

Exam 17: Procedures for Identifying Pathogens and Diagnosing Infections

Which technique used to diagnose a disease is also called the double diffusion method?

Broad general characteristics of bacteria on flowcharts can be misleading for a diagnosis of disease.

The Widal test is used for diagnosing salmonellosis.

Mary had an accidental needlestick one night during her shift as an emergency medical technician.Initially, she tested positive for HIV by the ELISA test.Which test would be done next to confirm an HIV infection?

A simple method to detect _____ cells involves mixing them with untreated sheep red blood cells that will bind and form rosettes.

Serological tests should have low sensitivity and specificity.

Which test is used to measure the IgE present in allergic patients?

Identify, describe, and discuss the objectives of the three main categories of identifying infectious agents using specific examples.

When antibodies or other substances in serum cross-react with the test reagents, a false positive result can occur.

Affixing antigen to an inert particle such as a latex bead is sometimes used in which type of test?

Serological testing always involves reactions between specific antibody and antigen.

Soluble antigens are detected in which type of test?

Why do many physicians feel that stool cultures are not necessary, except in certain circumstances when diagnosing GI tract infections?

In direct antigen testing, the reaction is generally seen with the:

When collecting __________, a "clean catch" method is often used.

The indirect ELISA test detects ______ in a patient's blood.

Serotyping of the pneumococcus based on capsular polysaccharide is performed in:

The more a sample can be diluted and yet still react with antigen, the ______ the concentration of antibodies in that sample and the _______ is its titer.

Which of the following choices are true about viral hemagglutination testing?

The Main Themes of Microbiology

The Chemistry of Biology

Tools of the Laboratory: Methods of Studying Microorganisms

A Survey of Prokaryotic Cells and Microorganisms

A Survey of Eukaryotic Cells and Microorganisms

An Introduction to Viruses

Microbial Nutrition, Ecology, and Growth

An Introduction to Microbial Metabolism: the Chemical Crossroads of Life

An Introduction to Microbial Genetics

Genetic Engineering: a Revolution in Molecular Biology

Physical and Chemical Agents for Microbial Control

Drugs, Microbes, Host-The Elements of Chemotherapy

Microbe-Human Interactions: Infection, Disease, and Epidemiology

An Introduction to Host Defenses and Innate Immunities

Adaptive, Specific Immunity and Immunization

Disorders in Immunity

The Gram-Positive and Gram-Negative Cocci of Medical Importance

The Gram-Positive Bacilli of Medical Importance

The Gram-Negative Bacilli of Medical Importance

Miscellaneous Bacterial Agents of Disease

The Fungi of Medical Importance

The Parasites of Medical Importance

Introduction to Viruses That Infect Humans: the Dna Viruses

The Rna Viruses That Infect Humans

Environmental Microbiology

Applied and Industrial Microbiology

Filters

The more a sample can be diluted and yet still react with antigen, the the concentration of antibodies in that sample and the _ is its titer.