Question 1

The tuberculin test is an example of an in vivo serological test.

Accepted Answer

A) True 
 B)False

Question 2

Which test is the confirming test for people who initially tested antibody-positive in the screening ELISA test?

Accepted Answer

A) Ouchterlony double diffusion 
B) Western blot 
C) immunelectrophoresis 
D) radioimmunoassay (RI
A)  
E) the Quellung test 
A) Ouchterlony double diffusion 
B) Western blot 
C) immunelectrophoresis 
D) radioimmunoassay (RI
A)  
E) the Quellung test

Question 3

Serotyping of the pneumococcus based on capsular polysaccharide is performed in:

Accepted Answer

A) Ouchterlony double diffusion. 
B) Western blot. 
C) immunelectrophoresis. 
D) radioimmunoassay (RI
A) . 
E) the Quellung test. 
A) Ouchterlony double diffusion. 
B) Western blot. 
C) immunelectrophoresis. 
D) radioimmunoassay (RI
A) . 
E) the Quellung test.

Question 4

When serum proteins are separated by electrophoresis and then antibodies specific for the serum proteins are placed in a parallel trough in order to form reaction arcs for each protein, the test is called:

Accepted Answer

A) Ouchterlony double diffusion. 
B) Western blot. 
C) immunelectrophoresis. 
D) radioimmunoassay (RI
A) . 
E) the Quellung test. 
A) Ouchterlony double diffusion. 
B) Western blot. 
C) immunelectrophoresis. 
D) radioimmunoassay (RI
A) . 
E) the Quellung test.

Question 5

When antibodies or other substances in serum cross-react with the test reagents, a false positive result can occur.

Accepted Answer

A) True 
 B)False

Question 6

All of the following are routine biochemical tests to aid in identifying a species, except:

Accepted Answer

A) motility. 
B) carbohydrate fermentation. 
C) catalase test. 
D) oxidase test. 
E) coagulase test. 
A) motility. 
B) carbohydrate fermentation. 
C) catalase test. 
D) oxidase test. 
E) coagulase test.

Question 7

When collecting __________, a "clean catch" method is often used.

Accepted Answer

The answer of When collecting __________, a "clean catch" method...

Question 8

Maggie was told she has a positive titer to measles.Which of the following could explain this?

Accepted Answer

A) She was vaccinated against measles. 
B) She had measles sometime in her life. 
C) She has measles now. 
D) The test was a false positive. 
E) All of the choices are correct. 
A) She was vaccinated against measles. 
B) She had measles sometime in her life. 
C) She has measles now. 
D) The test was a false positive. 
E) All of the choices are correct.

Question 9

In _________ tests, antibody cross-links whole-cell antigens, forming complexes that settle out and form visible clumps in the test chamber.

Accepted Answer

The answer of In _________ tests, antibody cross-links whole-cell antigens,...

Question 10

Precipitation tests involve all of the following, except:

Accepted Answer

A) they rely on formation of visible clumps for detection. 
B) they include the VDRL test for syphilis. 
C) they are often performed in agar gels. 
D) they can be done in a test tube by carefully adding antiserum over antigen solution. 
E) a cloudy or opaque zone developing where antigen and antibody react 
A) they rely on formation of visible clumps for detection. 
B) they include the VDRL test for syphilis. 
C) they are often performed in agar gels. 
D) they can be done in a test tube by carefully adding antiserum over antigen solution. 
E) a cloudy or opaque zone developing where antigen and antibody react

Question 11

A simple method to detect _____ cells involves mixing them with untreated sheep red blood cells that will bind and form rosettes.

Accepted Answer

A) True 
 B)False

Question 12

Soluble antigens are detected in which type of test?

Accepted Answer

A) cross-reactions 
B) agglutination 
C) precipitation 
D) specificity 
E) sensitivity 
A) cross-reactions 
B) agglutination 
C) precipitation 
D) specificity 
E) sensitivity

Question 13

Which of the following is involved in the in vitro diagnostic testing of serum?

Accepted Answer

A) mycology 
B) hematology 
C) serology 
D) histology 
E) virology 
A) mycology 
B) hematology 
C) serology 
D) histology 
E) virology

Question 14

ELISA tests use a fluorescent dye as a label to trace antigen-antibody reactions.

Accepted Answer

A) True 
 B)False

Question 15

Affixing antigen to an inert particle such as a latex bead is sometimes used in which type of test?

Accepted Answer

A) cross-reactions 
B) agglutination 
C) precipitation 
D) specificity 
E) sensitivity 
A) cross-reactions 
B) agglutination 
C) precipitation 
D) specificity 
E) sensitivity

Question 16

Sputum is obtained by:

Accepted Answer

A) swabbing the mouth to sample saliva. 
B) a clean catch urine sample. 
C) the patient coughing up mucus from the lower respiratory system. 
D) a spinal tap above the chest. 
E) a throat swab. 
A) swabbing the mouth to sample saliva. 
B) a clean catch urine sample. 
C) the patient coughing up mucus from the lower respiratory system. 
D) a spinal tap above the chest. 
E) a throat swab.

Question 17

Which of the following is incorrect regarding the complement fixation test?

Accepted Answer

A) First antigen and antibody are allowed to react. 
B) Purified complement proteins are added to the antigen-antibody tube. 
C) Sheep red blood cells are added to the antigen-antibody-complement mixture. 
D) Hemolysis of the sheep red blood cells occurs in a positive test result. 
E) None of the choices are correct. 
A) First antigen and antibody are allowed to react. 
B) Purified complement proteins are added to the antigen-antibody tube. 
C) Sheep red blood cells are added to the antigen-antibody-complement mixture. 
D) Hemolysis of the sheep red blood cells occurs in a positive test result. 
E) None of the choices are correct.

Question 18

The physician orders an antistreptolysin O (ASO) titer test to check for rheumatic fever.A patient serum sample is exposed to known suspensions of streptolysin and then allowed to incubate with RBCs? If the patient does have rheumatic fever, what will happen?

Accepted Answer

A) Not enough information is provided to speculate. 
B) The red blood cells will lyse. 
C) The red blood cells will agglutinate only. 
D) The red blood cells will not lyse. 
E) The red blood cells will fluoresce. 
A) Not enough information is provided to speculate. 
B) The red blood cells will lyse. 
C) The red blood cells will agglutinate only. 
D) The red blood cells will not lyse. 
E) The red blood cells will fluoresce.

Question 19

When antigen-antibody reactions are used to read a _____, they determine the quantity of antibodies in the serum.

Accepted Answer

The answer of When antigen-antibody reactions are used to read...

Question 20

In a __________, antigens or antibodies are labeled with radioactive isotopes and traced.

Accepted Answer

The answer of In a __________, antigens or antibodies are...

Exam 17: Procedures for Identifying Pathogens and Diagnosing Infections

The tuberculin test is an example of an in vivo serological test.

Which test is the confirming test for people who initially tested antibody-positive in the screening ELISA test?

Serotyping of the pneumococcus based on capsular polysaccharide is performed in:

When serum proteins are separated by electrophoresis and then antibodies specific for the serum proteins are placed in a parallel trough in order to form reaction arcs for each protein, the test is called:

When antibodies or other substances in serum cross-react with the test reagents, a false positive result can occur.

All of the following are routine biochemical tests to aid in identifying a species, except:

When collecting __________, a "clean catch" method is often used.

Maggie was told she has a positive titer to measles.Which of the following could explain this?

In _________ tests, antibody cross-links whole-cell antigens, forming complexes that settle out and form visible clumps in the test chamber.

Precipitation tests involve all of the following, except:

A simple method to detect _____ cells involves mixing them with untreated sheep red blood cells that will bind and form rosettes.

Soluble antigens are detected in which type of test?

Which of the following is involved in the in vitro diagnostic testing of serum?

ELISA tests use a fluorescent dye as a label to trace antigen-antibody reactions.

Affixing antigen to an inert particle such as a latex bead is sometimes used in which type of test?

Sputum is obtained by:

Which of the following is incorrect regarding the complement fixation test?

The physician orders an antistreptolysin O (ASO) titer test to check for rheumatic fever.A patient serum sample is exposed to known suspensions of streptolysin and then allowed to incubate with RBCs? If the patient does have rheumatic fever, what will happen?

When antigen-antibody reactions are used to read a _____, they determine the quantity of antibodies in the serum.

In a __________, antigens or antibodies are labeled with radioactive isotopes and traced.

The Main Themes of Microbiology

The Chemistry of Biology

Tools of the Laboratory: Methods of Studying Microorganisms

A Survey of Prokaryotic Cells and Microorganisms

A Survey of Eukaryotic Cells and Microorganisms

An Introduction to Viruses

Microbial Nutrition, Ecology, and Growth

An Introduction to Microbial Metabolism: the Chemical Crossroads of Life

Microbial Genetics

Genetic Engineering: a Revolution in Molecular Biology

Physical and Chemical Agents for Microbial Control

Drugs, Microbes, Host--The Elements of Chemotherapy

Microbe-Human Interactions: Infection and Disease

An Introduction to Host Defenses and Innate Immunities

Adaptive, Specific Immunity and Immunization

Disorders in Immunity

The Gram-Positive and Gram-Negative Cocci of Medical Importance

The Gram-Positive Bacilli of Medical Importance

The Gram-Negative Bacilli of Medical Importance

Miscellaneous Bacterial Agents of Disease

The Fungi of Medical Importance

The Parasites of Medical Importance

Introduction to Viruses That Infect Humans: the Dna Viruses

The Rna Viruses That Infect Humans

Environmental Microbiology

Applied and Industrial Microbiology

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