Question 1

Which of the following was used to create many of today's plasmid cloning vectors?

Accepted Answer

A)  pBR322 
B)  pGLO 
C)  pBAC 
D)  pGFP 
E)  pBLU 
A)  pBR322 
B)  pGLO 
C)  pBAC 
D)  pGFP 
E)  pBLU

Question 2

Which of the following can be determined using a cloned gene? 1.A gene's nucleotide sequence
2)A gene's function
3)A gene's amino acid sequence

Accepted Answer

A)  1 
B)  2 
C)  3 
D)  1 and 2 
E)  All of these 
A)  1 
B)  2 
C)  3 
D)  1 and 2 
E)  All of these

Question 3

What method allows bacteria to protect endogenous restriction sites from being cleaved by restriction enzymes?

Accepted Answer

A)  Methylation 
B)  Amylation 
C)  Glycosylation 
D)  Methylation and Amylation 
E)  None of these 
A)  Methylation 
B)  Amylation 
C)  Glycosylation 
D)  Methylation and Amylation 
E)  None of these

Question 4

In a gene cloning procedure the GFP gene is inserted into a plasmid and the plasmid is then taken up through transformation into an E.coli cell where it replicates.In which portion of this procedure is gene cloning actually taking place? 1.The GFP is inserted into the plasmid
2)The plasmid is taken up via transformation
3)The plasmid replicates in the E.coli cell

Accepted Answer

A)  1 
B)  2 
C)  3 
D)  1 and 2 
E)  2 and 3 
A)  1 
B)  2 
C)  3 
D)  1 and 2 
E)  2 and 3

Question 5

Which type of vector would be most effective for inserting a foreign piece of DNA that is 13kb in size into E.coli?

Accepted Answer

A)  Plasmid 
B)  Bacteriophage 
C)  BAC 
D)  Cosmid 
E)  YAC 
A)  Plasmid 
B)  Bacteriophage 
C)  BAC 
D)  Cosmid 
E)  YAC

Question 6

Which enzyme is used to join two complementary DNA fragments after a cut has been made?

Accepted Answer

A)  restriction endocnucleases 
B)  RNA polymerase 
C)  DNA gyrase 
D)  DNA ligase 
E)  Helicase 
A)  restriction endocnucleases 
B)  RNA polymerase 
C)  DNA gyrase 
D)  DNA ligase 
E)  Helicase

Question 7

Explain how genomic libraries are constructed and the two methods that are used to insert the DNA into the cloning vector.

Accepted Answer

Genomic DNA libraries are usually prepar

Question 8

Which of the following is true regarding restriction enzymes?
1)Methylation protects an organism's DNA from cutting by restriction enzymes
2)Restriction enzymes are exonucleases
3)Restriction enzymes can make staggered or blunt cuts

Accepted Answer

A)  1 
B)  2 
C)  3 
D)  1 and 2 
E)  1 and 3 
A)  1 
B)  2 
C)  3 
D)  1 and 2 
E)  1 and 3

Question 9

Which of the following statements about phagemid vectors is incorrect?

Accepted Answer

A)  They contain components from both phage chromosomes and plasmids 
B)  They require a helper phage to replicate and package single stranded DNA particles in phage heads 
C)  An example of a phagemid vector is pUC118 
D)  Can allow researchers to perform directional cloning 
E)  All of these 
A)  They contain components from both phage chromosomes and plasmids 
B)  They require a helper phage to replicate and package single stranded DNA particles in phage heads 
C)  An example of a phagemid vector is pUC118 
D)  Can allow researchers to perform directional cloning 
E)  All of these

Question 10

Which portion of the lambda phage can be excised and used for the insertion of foreign DNA?

Accepted Answer

A)  The external portions required for lytic growth 
B)  The central portion required for lysogenic growth 
C)  The external portions required for lysogenic growth 
D)  The central portion required for lytic growth 
E)  No part can be excised, but extra DNA can be added in 
A)  The external portions required for lytic growth 
B)  The central portion required for lysogenic growth 
C)  The external portions required for lysogenic growth 
D)  The central portion required for lytic growth 
E)  No part can be excised, but extra DNA can be added in

Question 11

Filamentous single-stranded DNA phages infect cells by absorbing to which of the following structures?

Accepted Answer

A)  Cell membrane 
B)  F pili 
C)  Cell wall 
D)  Nucleus 
E)  Mitochondria 
A)  Cell membrane 
B)  F pili 
C)  Cell wall 
D)  Nucleus 
E)  Mitochondria

Question 12

Site specific mutagenesis is commonly and efficiently performed by which procedure?

Accepted Answer

A)  PCR 
B)  Southern Blotting 
C)  Nothern Blotting 
D)  Western Blotting 
E)  Complementation Testing 
A)  PCR 
B)  Southern Blotting 
C)  Nothern Blotting 
D)  Western Blotting 
E)  Complementation Testing

Question 13

When mutations are available in a gene of interest,the wild-type allele of the gene can be identified by a process called:

Accepted Answer

A)  In vitro hybridization 
B)  Plaque hybridization 
C)  Southern hybridization 
D)  Complementation screening 
E)  In vitro packaging 
A)  In vitro hybridization 
B)  Plaque hybridization 
C)  Southern hybridization 
D)  Complementation screening 
E)  In vitro packaging

Question 14

Why is it possible to join a piece of DNA from a human that has been digested using EcoR1 and a piece of DNA from a bacterium that has been digested using EcoR1?

Accepted Answer

A)  They have been cut with the same restriction enzyme therefore the staggered ends are the same and are complementary 
B)  Bacteria and humans exist in a symbiotic relationship and therefore share DNA and can be joined together 
C)  They can join together only in the presence of high heat and pressure, such as is found in an autoclave. 
D)  They can not join together because the DNA has come from two different species and even though they are digested with the same restriction enzyme the cuts are at different locations. 
E)  All of these 
A)  They have been cut with the same restriction enzyme therefore the staggered ends are the same and are complementary 
B)  Bacteria and humans exist in a symbiotic relationship and therefore share DNA and can be joined together 
C)  They can join together only in the presence of high heat and pressure, such as is found in an autoclave. 
D)  They can not join together because the DNA has come from two different species and even though they are digested with the same restriction enzyme the cuts are at different locations. 
E)  All of these

Question 15

A researcher is using gel electrophoresis to separate very small DNA fragments.What type of gel should the researcher use to perform this separation?

Accepted Answer

A)  Agarose 
B)  Blood agar 
C)  Acrymalide 
D)  Agarose or Blood agar 
E)  All of these are appropriate 
A)  Agarose 
B)  Blood agar 
C)  Acrymalide 
D)  Agarose or Blood agar 
E)  All of these are appropriate

Question 16

How can site specific mutagenesis be conducted using PCR?

Accepted Answer

Site-specific mutagenesis is accomplishe

Question 17

Which of the following is not commonly used as a cloning vector?

Accepted Answer

A)  Plasmid 
B)  Cosmid 
C)  Phagemid 
D)  Bacteriphage 
E)  All of these are commonly used vectors 
A)  Plasmid 
B)  Cosmid 
C)  Phagemid 
D)  Bacteriphage 
E)  All of these are commonly used vectors

Which of the following was used to create many of today's plasmid cloning vectors?

Which of the following can be determined using a cloned gene? 1.A gene's nucleotide sequence 2)A gene's function 3)A gene's amino acid sequence

What method allows bacteria to protect endogenous restriction sites from being cleaved by restriction enzymes?

Which type of vector would be most effective for inserting a foreign piece of DNA that is 13kb in size into E.coli?

Which enzyme is used to join two complementary DNA fragments after a cut has been made?

Explain how genomic libraries are constructed and the two methods that are used to insert the DNA into the cloning vector.

Which of the following is true regarding restriction enzymes? 1)Methylation protects an organism's DNA from cutting by restriction enzymes 2)Restriction enzymes are exonucleases 3)Restriction enzymes can make staggered or blunt cuts

Which of the following statements about phagemid vectors is incorrect?

Which portion of the lambda phage can be excised and used for the insertion of foreign DNA?

Filamentous single-stranded DNA phages infect cells by absorbing to which of the following structures?

Site specific mutagenesis is commonly and efficiently performed by which procedure?

When mutations are available in a gene of interest,the wild-type allele of the gene can be identified by a process called:

Why is it possible to join a piece of DNA from a human that has been digested using EcoR1 and a piece of DNA from a bacterium that has been digested using EcoR1?

A researcher is using gel electrophoresis to separate very small DNA fragments.What type of gel should the researcher use to perform this separation?

How can site specific mutagenesis be conducted using PCR?

Which of the following is not commonly used as a cloning vector?

The Science Of Genetics

Cellular Reproduction

Mendelism The Basic Principles Of Inheritance

Extensions Of Mendelism

The Chromosomal Basis Of Mendelism

Variation In Chromosome Number And Structure

Linkage,Crossing Over,And Chromosome Mapping In …

The Genetics Of Bacteria And Their Viruses

DNA And The Molecular Structure Of Chromosomes

Replication Of DNA And Chromosomes

Transcription And RNA Processing

Translation And The Genetic Code

Mutation,DNA Repair,And Recombination

Genomics

Applications Of Molecular Genetics

Transposable Genetics Elements

Regulation Of Gene Expression In Prokaryotes

Regulation Of Gene Expression In Eukaryotes

The Genetic Control Of Animal Development

The Genetic Basis Of Cancer

Inheritance Of Complex Traits

Population Genetics

Evolutionary Genetics

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Exam 14: The Techniques Of Molecular Genetics

Which of the following was used to create many of today's plasmid cloning vectors?

Which of the following can be determined using a cloned gene? 1.A gene's nucleotide sequence 2)A gene's function 3)A gene's amino acid sequence

What method allows bacteria to protect endogenous restriction sites from being cleaved by restriction enzymes?

Which type of vector would be most effective for inserting a foreign piece of DNA that is 13kb in size into E.coli?

Which enzyme is used to join two complementary DNA fragments after a cut has been made?

Explain how genomic libraries are constructed and the two methods that are used to insert the DNA into the cloning vector.

Which of the following is true regarding restriction enzymes? 1)Methylation protects an organism's DNA from cutting by restriction enzymes 2)Restriction enzymes are exonucleases 3)Restriction enzymes can make staggered or blunt cuts

Which of the following statements about phagemid vectors is incorrect?

Which portion of the lambda phage can be excised and used for the insertion of foreign DNA?

Filamentous single-stranded DNA phages infect cells by absorbing to which of the following structures?

Site specific mutagenesis is commonly and efficiently performed by which procedure?

When mutations are available in a gene of interest,the wild-type allele of the gene can be identified by a process called:

Why is it possible to join a piece of DNA from a human that has been digested using EcoR1 and a piece of DNA from a bacterium that has been digested using EcoR1?

A researcher is using gel electrophoresis to separate very small DNA fragments.What type of gel should the researcher use to perform this separation?

How can site specific mutagenesis be conducted using PCR?

Which of the following is not commonly used as a cloning vector?

The Science Of Genetics

Cellular Reproduction

Mendelism The Basic Principles Of Inheritance

Extensions Of Mendelism

The Chromosomal Basis Of Mendelism

Variation In Chromosome Number And Structure

Linkage,Crossing Over,And Chromosome Mapping In …

The Genetics Of Bacteria And Their Viruses

DNA And The Molecular Structure Of Chromosomes

Replication Of DNA And Chromosomes

Transcription And RNA Processing

Translation And The Genetic Code

Mutation,DNA Repair,And Recombination

Genomics

Applications Of Molecular Genetics

Transposable Genetics Elements

Regulation Of Gene Expression In Prokaryotes

Regulation Of Gene Expression In Eukaryotes

The Genetic Control Of Animal Development

The Genetic Basis Of Cancer

Inheritance Of Complex Traits

Population Genetics

Evolutionary Genetics

Filters