Question 1

All of the following enzymes are used in the construction of cDNA libraries,except:

Accepted Answer

A)  Terminal transferase 
B)  Ribonuclease H 
C)  DNA polymerase I 
D)  DNA Ligase 
E)  Exonuclease 
A)  Terminal transferase 
B)  Ribonuclease H 
C)  DNA polymerase I 
D)  DNA Ligase 
E)  Exonuclease

Question 2

DNA molecules that are synthesized from an RNA template are known as:

Accepted Answer

A)  cDNA molecules 
B)  sDNA molecules 
C)  gDNA molecules 
D)  rDNA molecules 
E)  All of these 
A)  cDNA molecules 
B)  sDNA molecules 
C)  gDNA molecules 
D)  rDNA molecules 
E)  All of these

Question 3

Which of the following is a disadvantage of the PCR process?

Accepted Answer

A)  Fragments longer than 35kb cannot be efficiently amplified 
B)  It replicates double stranded DNA exponentially 
C)  It requires DNA to be denatured 
D)  All of these 
E)  None of these 
A)  Fragments longer than 35kb cannot be efficiently amplified 
B)  It replicates double stranded DNA exponentially 
C)  It requires DNA to be denatured 
D)  All of these 
E)  None of these

Question 4

Which of the following techniques is most appropriate for analyzing RNA fragments?

Accepted Answer

A)  Souther Blotting 
B)  Northern Blotting 
C)  RT-PCR 
D)  Southern Blotting and Northern Blotting 
E)  Northern Blotting and RT-PCR 
A)  Souther Blotting 
B)  Northern Blotting 
C)  RT-PCR 
D)  Southern Blotting and Northern Blotting 
E)  Northern Blotting and RT-PCR

Question 5

Which type of vector can replicate in both E.coli and another species?

Accepted Answer

A)  Plasmids 
B)  Lambda phage 
C)  Cosmids 
D)  Shuttle vectors 
E)  None of these 
A)  Plasmids 
B)  Lambda phage 
C)  Cosmids 
D)  Shuttle vectors 
E)  None of these

Question 6

Which of the following is found in a YAC?

Accepted Answer

A)  Yeast origin of replication 
B)  Two yeast telomeres at the end of the minichromosome 
C)  Yeast centromere 
D)  Polylinker site 
E)  All of these 
A)  Yeast origin of replication 
B)  Two yeast telomeres at the end of the minichromosome 
C)  Yeast centromere 
D)  Polylinker site 
E)  All of these

Question 7

What is the natural biological function of restriction endonucleases?

Accepted Answer

A)  To clone genes in eukaryotic sequences 
B)  To protect viruses from bacterial invasion 
C)  To protect bacteria from viral invasion 
D)  To protect eukaryotes from bacterial invasion 
E)  To clone genes in prokaryotic sequences 
A)  To clone genes in eukaryotic sequences 
B)  To protect viruses from bacterial invasion 
C)  To protect bacteria from viral invasion 
D)  To protect eukaryotes from bacterial invasion 
E)  To clone genes in prokaryotic sequences

Question 8

In a certain procedure,short DNA strands that are complementary to DNA sequences on either side of the gene or DNA sequence of interest are synthesized and used to initiate its amplification in vitro by a special (heat-stable)DNA polymerase.The procedure is known as:

Accepted Answer

A)  Telomerase Chain Reaction 
B)  Polymerase Chain Reaction 
C)  Gene cloning 
D)  DNA fingerprinting 
E)  Restriction Fragment Length Polymorphism 
A)  Telomerase Chain Reaction 
B)  Polymerase Chain Reaction 
C)  Gene cloning 
D)  DNA fingerprinting 
E)  Restriction Fragment Length Polymorphism

Question 9

Most of today's bacteriophage cloning vectors are derived from which phage?

Accepted Answer

A)  SV40 
B)  T4 
C)  Lambda 
D)  T2 
E)  pGLO 
A)  SV40 
B)  T4 
C)  Lambda 
D)  T2 
E)  pGLO

Question 10

What does the enzyme reverse transcriptase do?

Accepted Answer

A)  Degrades foreign DNA 
B)  Synthesizes RNA complementary to a DNA template 
C)  Synthesizes DNA complementary to an RNA template 
D)  Inactivates restriction endonucleases 
E)  Adds nucleotides to the end of DNA molecules 
A)  Degrades foreign DNA 
B)  Synthesizes RNA complementary to a DNA template 
C)  Synthesizes DNA complementary to an RNA template 
D)  Inactivates restriction endonucleases 
E)  Adds nucleotides to the end of DNA molecules

Question 11

Draw all of the DNA fragments that would result after 3 rounds of PCR if you start with: linear template DNA that is 2kb in length,two primers each of which have complementary sequences located 0.5 kb in from each end of the template and which are properly oriented for PCR,and all other components necessary for PCR.Label all DNA ends with regards to polarity and give fragment sizes where applicable.

Accepted Answer

The answer of Draw all of the DNA fragments that...

Question 12

What is a restriction endonuclease? Name and give a drawing of the types of ends that can be produced after restriction enzyme digestion.Why are these enzymes named restriction endonucleases?

Accepted Answer

A restriction endonuclease is an enzyme

Question 13

Which of the following is an essential component in a cloning vector?

Accepted Answer

A)  Origin of replication 
B)  Selectable marker gene 
C)  Unique restriction enzyme site 
D)  Origin of replication and Unique restriction enzyme site 
E)  All of these 
A)  Origin of replication 
B)  Selectable marker gene 
C)  Unique restriction enzyme site 
D)  Origin of replication and Unique restriction enzyme site 
E)  All of these

Question 14

In a gene cloning procedure the GFP gene is inserted into a plasmid and the plasmid is then taken up through transformation into an E.coli cell where it replicates.In which portion of this gene cloning procedure is the recombinant DNA molecule constructed? 1.The GFP is inserted into the plasmid
2)The plasmid is taken up via transformation
3)The plasmid replicates in the E.coli cell

Accepted Answer

A)  1 
B)  2 
C)  3 
D)  1 and 2 
E)  2 and 3 
A)  1 
B)  2 
C)  3 
D)  1 and 2 
E)  2 and 3

Question 15

Which of the following is not a feature of restriction enzymes?

Accepted Answer

A)  They recognize palindromic sequences 
B)  They make staggered cuts 
C)  They cut at random sites 
D)  They are named after the species in which the enzyme is produced 
E)  All of these are true 
A)  They recognize palindromic sequences 
B)  They make staggered cuts 
C)  They cut at random sites 
D)  They are named after the species in which the enzyme is produced 
E)  All of these are true

Question 16

Describe the Sanger method of DNA sequencing.

Accepted Answer

2¢,3¢-Dideoxyribonucleoside triphosphate

Question 17

A DNA molecule containing DNA fragments from two or more different sources is known as a:

Accepted Answer

A)  Recombinant DNA molecule 
B)  Mutated DNA molecule 
C)  Biologically normal DNA molecule 
D)  Wild type DNA molecule 
E)  Homeric DNA molecule 
A)  Recombinant DNA molecule 
B)  Mutated DNA molecule 
C)  Biologically normal DNA molecule 
D)  Wild type DNA molecule 
E)  Homeric DNA molecule

Question 18

Why is Taq polymerase used more commonly in PCR than DNA polymerase I from E.coli?
1)Taq polymerase remains active during the high heat denaturation step unlike DNA polymerase I
2)Taq polymerase is less expensive than DNA polymerase I
3)Taq polymerase must be added after each deanturation step unlike DNA polymerase I

Accepted Answer

A)  1 
B)  2 
C)  3 
D)  1 and 2 
E)  All of these 
A)  1 
B)  2 
C)  3 
D)  1 and 2 
E)  All of these

Question 19

What is the genetic sequence shown on the autoradiogram below?

Accepted Answer

A)  AATGTCAGCCTT 
B)  TTCCGACTGTAA 
C)  AAATTTTCCCGG 
D)  GGCCCTTTTAAA 
E)  TTTAAAAGGGCC 
A)  AATGTCAGCCTT 
B)  TTCCGACTGTAA 
C)  AAATTTTCCCGG 
D)  GGCCCTTTTAAA 
E)  TTTAAAAGGGCC

Question 20

How is a Southern Blot performed and why does this technique provide more information than gel electrophoresis?

Accepted Answer

While gel electrophoresis allows one to

Exam 14: The Techniques Of Molecular Genetics

All of the following enzymes are used in the construction of cDNA libraries,except:

DNA molecules that are synthesized from an RNA template are known as:

Which of the following is a disadvantage of the PCR process?

Which of the following techniques is most appropriate for analyzing RNA fragments?

Which type of vector can replicate in both E.coli and another species?

Which of the following is found in a YAC?

What is the natural biological function of restriction endonucleases?

In a certain procedure,short DNA strands that are complementary to DNA sequences on either side of the gene or DNA sequence of interest are synthesized and used to initiate its amplification in vitro by a special (heat-stable)DNA polymerase.The procedure is known as:

Most of today's bacteriophage cloning vectors are derived from which phage?

What does the enzyme reverse transcriptase do?

What is a restriction endonuclease? Name and give a drawing of the types of ends that can be produced after restriction enzyme digestion.Why are these enzymes named restriction endonucleases?

Which of the following is an essential component in a cloning vector?

Which of the following is not a feature of restriction enzymes?

Describe the Sanger method of DNA sequencing.

A DNA molecule containing DNA fragments from two or more different sources is known as a:

What is the genetic sequence shown on the autoradiogram below?

How is a Southern Blot performed and why does this technique provide more information than gel electrophoresis?

The Science Of Genetics

Cellular Reproduction

Mendelism The Basic Principles Of Inheritance

Extensions Of Mendelism

The Chromosomal Basis Of Mendelism

Variation In Chromosome Number And Structure

Linkage,Crossing Over,And Chromosome Mapping In …

The Genetics Of Bacteria And Their Viruses

DNA And The Molecular Structure Of Chromosomes

Replication Of DNA And Chromosomes

Transcription And RNA Processing

Translation And The Genetic Code

Mutation,DNA Repair,And Recombination

Genomics

Applications Of Molecular Genetics

Transposable Genetics Elements

Regulation Of Gene Expression In Prokaryotes

Regulation Of Gene Expression In Eukaryotes

The Genetic Control Of Animal Development

The Genetic Basis Of Cancer

Inheritance Of Complex Traits

Population Genetics

Evolutionary Genetics

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