Question 1

The spliceosome

Accepted Answer

A)  is a cis-acting ribozyme. 
B)  resembles group II introns in its mechanism and product. 
C)  performs the same function in prokaryotes and eukaryotes. 
D)  is a protein enzyme. 
A)  is a cis-acting ribozyme. 
B)  resembles group II introns in its mechanism and product. 
C)  performs the same function in prokaryotes and eukaryotes. 
D)  is a protein enzyme. B

Question 2

Approximately how many different types of RNA promotors are needed in eukaryotic systems?

Accepted Answer

A)  1 
B)  2 
C)  3 
D)  4 
A)  1 
B)  2 
C)  3 
D)  4 C

Question 3

Antisense RNA binds to __________ and sense RNA binds to __________.

Accepted Answer

A)  template DNA; coding DNA 
B)  tRNA; mRNA 
C)  coding DNA; template DNA 
D)  mRNA; coding DNA 
A)  template DNA; coding DNA 
B)  tRNA; mRNA 
C)  coding DNA; template DNA 
D)  mRNA; coding DNA A

Question 4

Long noncoding RNA (lncRNA)is generated from

Accepted Answer

A)  the tips of chromosomes. 
B)  the transcription of genomic DNA. 
C)  the splicing of used mRNA. 
D)  an infection of viral RNA. 
A)  the tips of chromosomes. 
B)  the transcription of genomic DNA. 
C)  the splicing of used mRNA. 
D)  an infection of viral RNA.

Question 5

Which is the correct order of mRNA degradation?

Accepted Answer

A)  (1) poly(
A)  tail removal; (2) 7-methylguanosine cap removal; (3) RNA hydrolysis 
B)  (1) 7-methylguanosine cap removal; (2) RNA hydrolysis; (3) poly(
A)  tail removal 
C)  (1) 7-methylguanosine cap removal; (2) poly(
A)  tail removal; (3) RNA hydrolysis 
D)  (1) RNA hydrolysis; (2) Poly(
A)  tail removal; (3) 7-methylguanosine cap removal 
A)  (1) poly(
A)  tail removal; (2) 7-methylguanosine cap removal; (3) RNA hydrolysis 
B)  (1) 7-methylguanosine cap removal; (2) RNA hydrolysis; (3) poly(
A)  tail removal 
C)  (1) 7-methylguanosine cap removal; (2) poly(
A)  tail removal; (3) RNA hydrolysis 
D)  (1) RNA hydrolysis; (2) Poly(
A)  tail removal; (3) 7-methylguanosine cap removal

Question 6

What are   factors in RNA synthesis?

Accepted Answer

A)  DNA promoter regions 
B)  RNA polymerases 
C)  transcription factors 
D)  receptor proteins 
A)  DNA promoter regions 
B)  RNA polymerases 
C)  transcription factors 
D)  receptor proteins

Question 7

Describe two ways that termination can occur in the transcription of prokaryotic genes.

Accepted Answer

(1)The protein Rho binds to a C-rich reg

Question 8

A knockout mutation in the X-inactive specific transcript (XIST)lncRNA of mice would result in

Accepted Answer

A)  one inactivated X chromosome in female mice. 
B)  the inhibited transcription and translation of X-linked proteins. 
C)  two inactive X chromosomes in female mice. 
D)  two active X chromosomes in female mice. 
A)  one inactivated X chromosome in female mice. 
B)  the inhibited transcription and translation of X-linked proteins. 
C)  two inactive X chromosomes in female mice. 
D)  two active X chromosomes in female mice.

Question 9

Describe the composition and processing of the C-terminal domain (CTD)of eukaryotic RNA polymerase II,as well as its role in transcription.

Accepted Answer

The CTD is a repeating peptide sequence

Question 10

Which of the following is a coding RNA molecule?

Accepted Answer

A)  short interfering RNA. 
B)  TERC RNA. 
C)  small nucleolar RNA. 
D)  messenger RNA. 
A)  short interfering RNA. 
B)  TERC RNA. 
C)  small nucleolar RNA. 
D)  messenger RNA.

Question 11

A 7-methylguanylate cap and poly(A)tail is added to mRNA to

Accepted Answer

A)  differentiate the mRNA from the tRNA. 
B)  facilitate binding and translation by the ribosome. 
C)  increase mRNA splicing efficiency. 
D)  signify the start and end of the gene sequence. 
A)  differentiate the mRNA from the tRNA. 
B)  facilitate binding and translation by the ribosome. 
C)  increase mRNA splicing efficiency. 
D)  signify the start and end of the gene sequence.

Question 12

The enzyme responsible for making siRNA is

Accepted Answer

A)  RISC. 
B)  snoRNA. 
C)  exosome. 
D)  dicer. 
A)  RISC. 
B)  snoRNA. 
C)  exosome. 
D)  dicer.

Question 13

Eukaryotic promoters,but NOT prokaryotic promoters,

Accepted Answer

A)  are located before the transcription start site. 
B)  bind transcription factors. 
C)  are located after the transcription start site. 
D)  assist in activating transcription. 
A)  are located before the transcription start site. 
B)  bind transcription factors. 
C)  are located after the transcription start site. 
D)  assist in activating transcription.

Question 14

Which enzyme is responsible for adding the poly(A)tail to mRNA?

Accepted Answer

A)  cleavage stimulatory factor (CStF) 
B)  poly(
A)  polymerase 
C)  cleavage and polyadenylation specificity factor (CPSF) 
D)  poly(
A)  binding protein 
A)  cleavage stimulatory factor (CStF) 
B)  poly(
A)  polymerase 
C)  cleavage and polyadenylation specificity factor (CPSF) 
D)  poly(
A)  binding protein

Question 15

Which is NOT required in the prokaryotic transcription initiation complex?

Accepted Answer

A)  a primer 
B)  RNA polymerase 
C)  initiation factor 
D)  a ribonucleotide triphosphate (NTP) 
A)  a primer 
B)  RNA polymerase 
C)  initiation factor 
D)  a ribonucleotide triphosphate (NTP)

Question 16

The initiation of transcription in eukaryotes

Accepted Answer

A)  occurs in the same manner as prokaryotic transcription. 
B)  requires the complete unfolding of the gene into single-stranded DNA. 
C)  requires many more transcription factors than prokaryotic transcription. 
D)  uses RNA polymerase as well as helicase and primase. 
A)  occurs in the same manner as prokaryotic transcription. 
B)  requires the complete unfolding of the gene into single-stranded DNA. 
C)  requires many more transcription factors than prokaryotic transcription. 
D)  uses RNA polymerase as well as helicase and primase.

Question 17

Group I and group II introns are similar in their

Accepted Answer

A)  requirement for metal ion cofactors. 
B)  requirement for exogenous nucleoside binding. 
C)  DNA composition. 
D)  intron lariat structure. 
A)  requirement for metal ion cofactors. 
B)  requirement for exogenous nucleoside binding. 
C)  DNA composition. 
D)  intron lariat structure.

Question 18

Eukaryotic RNA polymerase

Accepted Answer

A)  is a ribozyme. 
B)  contains as many as five protein subunits in the final functional enzyme. 
C)  synthesizes all RNA types. 
D)  has a common core structure resembling that of prokaryotic RNA polymerase. 
A)  is a ribozyme. 
B)  contains as many as five protein subunits in the final functional enzyme. 
C)  synthesizes all RNA types. 
D)  has a common core structure resembling that of prokaryotic RNA polymerase.

Question 19

Eukaryotic transcription promoters

Accepted Answer

A)  require multiple DNA binding regions. 
B)  are largely conserved across the genome. 
C)  can stimulate DNA polymerases by direct binding. 
D)  control translational as well as transcriptional events. 
A)  require multiple DNA binding regions. 
B)  are largely conserved across the genome. 
C)  can stimulate DNA polymerases by direct binding. 
D)  control translational as well as transcriptional events.

Question 20

Why must tRNA contain base modifications?

Accepted Answer

tRNAs have very similar second

Exam 21: Rna Synthesis, processing, and Gene Silencing

The spliceosome

Approximately how many different types of RNA promotors are needed in eukaryotic systems?

Antisense RNA binds to __________ and sense RNA binds to __________.

Long noncoding RNA (lncRNA)is generated from

Which is the correct order of mRNA degradation?

What are factors in RNA synthesis?

Describe two ways that termination can occur in the transcription of prokaryotic genes.

A knockout mutation in the X-inactive specific transcript (XIST)lncRNA of mice would result in

Describe the composition and processing of the C-terminal domain (CTD)of eukaryotic RNA polymerase II,as well as its role in transcription.

Which of the following is a coding RNA molecule?

A 7-methylguanylate cap and poly(A)tail is added to mRNA to

The enzyme responsible for making siRNA is

Eukaryotic promoters,but NOT prokaryotic promoters,

Which enzyme is responsible for adding the poly(A)tail to mRNA?

Which is NOT required in the prokaryotic transcription initiation complex?

The initiation of transcription in eukaryotes

Group I and group II introns are similar in their

Eukaryotic RNA polymerase

Eukaryotic transcription promoters

Why must tRNA contain base modifications?

Principles of Biochemistry

Physical Biochemistry: Energy Conversion,water,and Membranes

Nucleic Acid Structure and Function

Protein Structure

Methods in Protein Biochemistry

Protein Function

Enzyme Mechanisms

Cell Signaling Systems

Glycolysis: a Paradigm of Metabolic Regulation

The Citrate Cycle

Oxidative Phosphorylation

Photosynthesis

Carbohydrate Structure and Function

Carbohydrate Metabolism

Lipid Structure and Function

Lipid Metabolism

Amino Acid Metabolism

Nucleotide Metabolism

Metabolic Integration

Dna Replication, repair, and Recombination

Protein Synthesis, posttranslational Modification, and Transport

Gene Regulation

Filters

Antisense RNA binds to and sense RNA binds to .