Question 1

Which peptide would migrate the fastest in a SDS-PAGE gel?

Accepted Answer

A)  A 
B)  B 
C)  C 
D)  D 
A)  A 
B)  B 
C)  C 
D)  D A

Question 2

After using the salting out method to isolate proteins,how is ammonium sulfate removed from the solution?

Accepted Answer

A)  sonication 
B)  French press 
C)  dialysis 
D)  centrifugation 
A)  sonication 
B)  French press 
C)  dialysis 
D)  centrifugation C

Question 3

Which animal is most often used to generate monoclonal antibodies?

Accepted Answer

A)  rabbit 
B)  chicken 
C)  goat 
D)  mouse 
A)  rabbit 
B)  chicken 
C)  goat 
D)  mouse D

Question 4

Most secondary antibodies used in Western blots are covalently linked to the enzyme

Accepted Answer

A)  luciferase. 
B)  horseradish peroxidase. 
C)  catalase. 
D)  hydrolase. 
A)  luciferase. 
B)  horseradish peroxidase. 
C)  catalase. 
D)  hydrolase.

Question 5

Predict the fragments of the following peptide after cleavage by the V-8 protease. CMVAADGKLEPVS

Accepted Answer

A)  CMVAAD GKLEPVS 
B)  CMVAADGKLE PVS 
C)  CMVAADG KLEPVS 
D)  CMVAAD GKLE PVS 
A)  CMVAAD GKLEPVS 
B)  CMVAADGKLE PVS 
C)  CMVAADG KLEPVS 
D)  CMVAAD GKLE PVS

Question 6

Immunoprecipitation uses an antibody-bead combination to

Accepted Answer

A)  detect the antigen. 
B)  enrich the protein solution. 
C)  digest the proteins for use in mass spectrometry. 
D)  separate the proteins using centrifugation. 
A)  detect the antigen. 
B)  enrich the protein solution. 
C)  digest the proteins for use in mass spectrometry. 
D)  separate the proteins using centrifugation.

Question 7

The part of the Western blot that contains the protein-specific recognition and facilitates the antigen-antibody interactions is the

Accepted Answer

A)  SDS-PAGE gel. 
B)  primary antibody. 
C)  tertiary antibody. 
D)  filter membrane. 
A)  SDS-PAGE gel. 
B)  primary antibody. 
C)  tertiary antibody. 
D)  filter membrane.

Question 8

The specific sites on the antigen that interacts with the antibody are called

Accepted Answer

A)  epitopes. 
B)  immunoglobin light chains. 
C)  immunoglobin heavy chains. 
D)  variable-domain amino acid residues. 
A)  epitopes. 
B)  immunoglobin light chains. 
C)  immunoglobin heavy chains. 
D)  variable-domain amino acid residues.

Question 9

Calculate the specific activity when 500 mg of protein has an activity of 18,000 units.

Accepted Answer

A)  0.28 units/mg protein 
B)  36 units/mg protein 
C)  9000 units/mg protein 
D)  13,000 units/mg protein 
A)  0.28 units/mg protein 
B)  36 units/mg protein 
C)  9000 units/mg protein 
D)  13,000 units/mg protein

Question 10

Which component used in a Western blot is covalently linked to an enzyme?

Accepted Answer

A)  SDS-PAGE gel 
B)  primary antibody 
C)  secondary antibody 
D)  filter membrane 
A)  SDS-PAGE gel 
B)  primary antibody 
C)  secondary antibody 
D)  filter membrane

Question 11

In an ELISA,the detection antibody is a __________ antibody.

Accepted Answer

A)  monoclonal primary 
B)  polyclonal tertiary 
C)  monoclonal secondary 
D)  polyclonal secondary 
A)  monoclonal primary 
B)  polyclonal tertiary 
C)  monoclonal secondary 
D)  polyclonal secondary

Question 12

The Fab immunoglobin fragment

Accepted Answer

A)  is generally found within  -barrels. 
B)  is located in polar surface groups in loop regions. 
C)  contains the high-affinity antigen binding site. 
D)  is responsible for the solubility of the antibody. 
A)  is generally found within  -barrels. 
B)  is located in polar surface groups in loop regions. 
C)  contains the high-affinity antigen binding site. 
D)  is responsible for the solubility of the antibody.

Question 13

What are the advantages of using a monoclonal antibody compared with a polyclonal antibody?

Accepted Answer

Monoclonal antibodies are generated from

Question 14

Explain how protein samples that contain the antigenic protein are recognized using a Western blot.

Accepted Answer

The proteins are separated using gel ele

Question 15

Polyclonal antibodies are

Accepted Answer

A)  derived from B cells isolated from the spleen. 
B)  isolated from hybridoma cells. 
C)  a heterogeneous mixture. 
D)  a homogenous mixture. 
A)  derived from B cells isolated from the spleen. 
B)  isolated from hybridoma cells. 
C)  a heterogeneous mixture. 
D)  a homogenous mixture.

Question 16

Protein-specific antibodies can be used to detect proteins within cells conserving the cell architecture using

Accepted Answer

A)  immunofluorescence. 
B)  Western blot. 
C)  immunoprecipitation. 
D)  ELISA. 
A)  immunofluorescence. 
B)  Western blot. 
C)  immunoprecipitation. 
D)  ELISA.

Question 17

Why is protein purification often referred to as an art and a science?

Accepted Answer

There are between 10,000 and 100,000 dif

Question 18

A polypeptide was digested by trypsin and chymotrypsin.Use the following information to determine the polypeptide sequence.

Accepted Answer

A)  ECFGMREDWKYLM 
B)  LMYKWMGFCEDER 
C)  LMYKWDERMGFCE 
D)  CELMYKWDERMGF 
A)  ECFGMREDWKYLM 
B)  LMYKWMGFCEDER 
C)  LMYKWDERMGFCE 
D)  CELMYKWDERMGF

Question 19

The figure below shows how differential centrifugation can be used to isolate four subcellular fractions.Which fraction corresponds to the membrane fraction?

Accepted Answer

A)  A 
B)  B 
C)  C 
D)  D 
A)  A 
B)  B 
C)  C 
D)  D

Question 20

Which percentage of polyacrylamide would give the best separation for large proteins?

Accepted Answer

A)  5% 
B)  7.5% 
C)  10% 
D)  20% 
A)  5% 
B)  7.5% 
C)  10% 
D)  20%

Exam 5: Methods in Protein Biochemistry

Which peptide would migrate the fastest in a SDS-PAGE gel?

After using the salting out method to isolate proteins,how is ammonium sulfate removed from the solution?

Which animal is most often used to generate monoclonal antibodies?

Most secondary antibodies used in Western blots are covalently linked to the enzyme

Predict the fragments of the following peptide after cleavage by the V-8 protease. CMVAADGKLEPVS

Immunoprecipitation uses an antibody-bead combination to

The part of the Western blot that contains the protein-specific recognition and facilitates the antigen-antibody interactions is the

The specific sites on the antigen that interacts with the antibody are called

Calculate the specific activity when 500 mg of protein has an activity of 18,000 units.

Which component used in a Western blot is covalently linked to an enzyme?

In an ELISA,the detection antibody is a __________ antibody.

The Fab immunoglobin fragment

What are the advantages of using a monoclonal antibody compared with a polyclonal antibody?

Explain how protein samples that contain the antigenic protein are recognized using a Western blot.

Polyclonal antibodies are

Protein-specific antibodies can be used to detect proteins within cells conserving the cell architecture using

Why is protein purification often referred to as an art and a science?

A polypeptide was digested by trypsin and chymotrypsin.Use the following information to determine the polypeptide sequence.

The figure below shows how differential centrifugation can be used to isolate four subcellular fractions.Which fraction corresponds to the membrane fraction?

Which percentage of polyacrylamide would give the best separation for large proteins?

Principles of Biochemistry

Physical Biochemistry: Energy Conversion,water,and Membranes

Nucleic Acid Structure and Function

Protein Structure

Protein Function

Enzyme Mechanisms

Cell Signaling Systems

Glycolysis: a Paradigm of Metabolic Regulation

The Citrate Cycle

Oxidative Phosphorylation

Photosynthesis

Carbohydrate Structure and Function

Carbohydrate Metabolism

Lipid Structure and Function

Lipid Metabolism

Amino Acid Metabolism

Nucleotide Metabolism

Metabolic Integration

Dna Replication, repair, and Recombination

Rna Synthesis, processing, and Gene Silencing

Protein Synthesis, posttranslational Modification, and Transport

Gene Regulation

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