Question 1

Eukaryotic RNA polymerase II facilitates the

Accepted Answer

A)  removal of exons. 
B)  addition of 7-methylguanylate cap. 
C)  addition of a poly(
A)  tail. 
D)  translation of the RNA transcript. 
A)  removal of exons. 
B)  addition of 7-methylguanylate cap. 
C)  addition of a poly(
A)  tail. 
D)  translation of the RNA transcript.

Question 2

Describe what is depicted in the figure below and explain the role of Ras-related nuclear protein (Ran).

Accepted Answer

The different classes of RNAs are transp

Question 3

Which type of RNA facilitates RNA interference by resulting in degraded mRNA?

Accepted Answer

A)  snoRNA 
B)  siRNA 
C)  miRNA 
D)  rRNA 
A)  snoRNA 
B)  siRNA 
C)  miRNA 
D)  rRNA

Question 4

Which enzyme is important in the processing of tRNA and mRNA from prokaryotes?

Accepted Answer

A)  RNaseP 
B)  snRNA 
C)  reverse transcriptase 
D)  ribozyme 
A)  RNaseP 
B)  snRNA 
C)  reverse transcriptase 
D)  ribozyme

Question 5

The most significant influence on why mRNA is processed differently in prokaryotes than eukaryotes is the fact that

Accepted Answer

A)  eukaryotes separate transcription and translation with a nucleus. 
B)  prokaryotes are often polycistronic. 
C)  eukaryotes are multicellular organisms. 
D)  prokaryotes do not add a poly(
A)  tail. 
A)  eukaryotes separate transcription and translation with a nucleus. 
B)  prokaryotes are often polycistronic. 
C)  eukaryotes are multicellular organisms. 
D)  prokaryotes do not add a poly(
A)  tail.

Question 6

What do group I introns require that group II introns do not?

Accepted Answer

A)  ATP hydrolysis 
B)  metal ion cofactors 
C)  guanosine cofactor binding 
D)  external RNA substrate 
A)  ATP hydrolysis 
B)  metal ion cofactors 
C)  guanosine cofactor binding 
D)  external RNA substrate

Question 7

Different mRNAs can be obtained from the same gene by

Accepted Answer

A)  terminating transcription at different stop codons. 
B)  splicing the mRNA with different exons. 
C)  adding the 7-methylguanosine cap at different sites. 
D)  alternate folding of mRNA. 
A)  terminating transcription at different stop codons. 
B)  splicing the mRNA with different exons. 
C)  adding the 7-methylguanosine cap at different sites. 
D)  alternate folding of mRNA.

Question 8

In E.coli,__________ RNA-DNA base pairs are maintained and about __________ base pairs of single-stranded DNA are maintained in the transcription bubble.

Accepted Answer

A)  20; 80 
B)  8; 17 
C)  17; 20 
D)  200; 1000 
A)  20; 80 
B)  8; 17 
C)  17; 20 
D)  200; 1000

Question 9

Which class of RNA molecules is unique to eukaryotic organisms?

Accepted Answer

A)  transfer RNA 
B)  ribosomal RNA 
C)  small nuclear RNA 
D)  messenger RNA 
A)  transfer RNA 
B)  ribosomal RNA 
C)  small nuclear RNA 
D)  messenger RNA

Question 10

List the classes and types of noncoding RNAs that are found typically in eukaryotic organisms.

Accepted Answer

Short noncoding RNAs include (1)micro RN

Question 11

What is the biggest difference between miRNA and siRNA?

Accepted Answer

A)  One is derived from the nucleus and one is derived from double-stranded RNA. 
B)  One regulates gene expression, whereas the other regulates protein synthesis. 
C)  One binds to mRNA and suppresses its translation and one binds to mRNA and signals degradation. 
D)  One binds to the DNA gene and inhibits transcription and one binds to the mRNA and signals degradation. 
A)  One is derived from the nucleus and one is derived from double-stranded RNA. 
B)  One regulates gene expression, whereas the other regulates protein synthesis. 
C)  One binds to mRNA and suppresses its translation and one binds to mRNA and signals degradation. 
D)  One binds to the DNA gene and inhibits transcription and one binds to the mRNA and signals degradation.

Question 12

The __________ strand of DNA is transcribed into mRNA.

Accepted Answer

A)  leading 
B)  coding 
C)  lagging 
D)  template 
A)  leading 
B)  coding 
C)  lagging 
D)  template

Question 13

What best describes the composition of the spliceosome?

Accepted Answer

A)  small nuclear ribonucleoprotein 
B)  RNA only 
C)  protein 
D)  mRNA 
A)  small nuclear ribonucleoprotein 
B)  RNA only 
C)  protein 
D)  mRNA

Question 14

Which enzyme degrades the poly(A)tail in the degradation of mRNA?

Accepted Answer

A)  poly(
A)  binding protein 
B)  exosome 
C)  CCR4 
D)  DCP1 and DCP2 
A)  poly(
A)  binding protein 
B)  exosome 
C)  CCR4 
D)  DCP1 and DCP2

Question 15

Which is often removed from eukaryotic mRNA before translation?

Accepted Answer

A)  poly(
A)  tails 
B)  exons 
C)  polycistrons 
D)  introns 
A)  poly(
A)  tails 
B)  exons 
C)  polycistrons 
D)  introns

Question 16

The information gained from the DNA footprinting technique is the

Accepted Answer

A)  DNA sequence. 
B)  promotor region of a gene. 
C)  location of a gene in DNA. 
D)  location of a DNA binding protein on DNA. 
A)  DNA sequence. 
B)  promotor region of a gene. 
C)  location of a gene in DNA. 
D)  location of a DNA binding protein on DNA.

Question 17

What is a difference between group I and group II introns?

Accepted Answer

A)  intron cleaving versus exon ligating abilities 
B)  cis versus trans cleaving capabilities 
C)  linear versus lariat intron products 
D)  nucleophilic versus electrophilic hydroxyl attacks 
A)  intron cleaving versus exon ligating abilities 
B)  cis versus trans cleaving capabilities 
C)  linear versus lariat intron products 
D)  nucleophilic versus electrophilic hydroxyl attacks

Question 18

Describe the timing and location of removal of introns and splicing of exons in mRNA.

Accepted Answer

After the addition of the 7-methylguanos

Question 19

Which class of RNA molecule is typically the shortest in length?

Accepted Answer

A)  transfer RNA 
B)  messenger RNA 
C)  small nuclear RNA 
D)  micro RNA 
A)  transfer RNA 
B)  messenger RNA 
C)  small nuclear RNA 
D)  micro RNA

Question 20

miRNA is

Accepted Answer

A)  involved in stimulating gene expression. 
B)  derived from degraded mRNA. 
C)  expressed in the nucleus and is involved in regulating gene expression. 
D)  derived from degraded virus RNA strands. 
A)  involved in stimulating gene expression. 
B)  derived from degraded mRNA. 
C)  expressed in the nucleus and is involved in regulating gene expression. 
D)  derived from degraded virus RNA strands.

Exam 21: Rna Synthesis, processing, and Gene Silencing

Eukaryotic RNA polymerase II facilitates the

Describe what is depicted in the figure below and explain the role of Ras-related nuclear protein (Ran).

Which type of RNA facilitates RNA interference by resulting in degraded mRNA?

Which enzyme is important in the processing of tRNA and mRNA from prokaryotes?

The most significant influence on why mRNA is processed differently in prokaryotes than eukaryotes is the fact that

What do group I introns require that group II introns do not?

Different mRNAs can be obtained from the same gene by

In E.coli,__________ RNA-DNA base pairs are maintained and about __________ base pairs of single-stranded DNA are maintained in the transcription bubble.

Which class of RNA molecules is unique to eukaryotic organisms?

List the classes and types of noncoding RNAs that are found typically in eukaryotic organisms.

What is the biggest difference between miRNA and siRNA?

The __________ strand of DNA is transcribed into mRNA.

What best describes the composition of the spliceosome?

Which enzyme degrades the poly(A)tail in the degradation of mRNA?

Which is often removed from eukaryotic mRNA before translation?

The information gained from the DNA footprinting technique is the

What is a difference between group I and group II introns?

Describe the timing and location of removal of introns and splicing of exons in mRNA.

Which class of RNA molecule is typically the shortest in length?

miRNA is

Principles of Biochemistry

Physical Biochemistry: Energy Conversion,water,and Membranes

Nucleic Acid Structure and Function

Protein Structure

Methods in Protein Biochemistry

Protein Function

Enzyme Mechanisms

Cell Signaling Systems

Glycolysis: a Paradigm of Metabolic Regulation

The Citrate Cycle

Oxidative Phosphorylation

Photosynthesis

Carbohydrate Structure and Function

Carbohydrate Metabolism

Lipid Structure and Function

Lipid Metabolism

Amino Acid Metabolism

Nucleotide Metabolism

Metabolic Integration

Dna Replication, repair, and Recombination

Protein Synthesis, posttranslational Modification, and Transport

Gene Regulation

Filters

In E.coli, RNA-DNA base pairs are maintained and about base pairs of single-stranded DNA are maintained in the transcription bubble.