Question 1

A one nucleotide slip in the splice point on the side of the splice junction in the first intron of the beta-globin gene would result in

Accepted Answer

A)  changing the reading frame of the mRNA from this point onward. 
B)  deletion of the rest of the beta-globin gene. 
C)  no change in the protein as all coding information is 5' to this junction. 
D)  a transition mutation. 
E)  a transversion mutation. 
A)  changing the reading frame of the mRNA from this point onward. 
B)  deletion of the rest of the beta-globin gene. 
C)  no change in the protein as all coding information is 5' to this junction. 
D)  a transition mutation. 
E)  a transversion mutation.

Question 2

Synthesis of the precursor-mRNA molecule of various enzymes occurs in

Accepted Answer

A)  nucleolus. 
B)  nucleus (excluding the nucleolus). 
C)  cytosol. 
D)  Golgi apparatus. 
E)  rough endoplasmic reticulum. 
A)  nucleolus. 
B)  nucleus (excluding the nucleolus). 
C)  cytosol. 
D)  Golgi apparatus. 
E)  rough endoplasmic reticulum.

Question 3

Which statement is true?

Accepted Answer

A)  transcription of DNA to RNA initiates at a region of the DNA called a promoter region. 
B)  Within a given gene, usually one strand of the DNA is copied and then the other is copied into RNA. 
C)  When DNA or RNA is copied this means that from the DNA sequence GCAGTTA the copy will read GCAGTTA. 
D)  During DNA synthesis, the primer is copied into RNA but is later removed by an exonuclease. 
E)  The 5' end of a DNA molecule always has the phosphate. 
A)  transcription of DNA to RNA initiates at a region of the DNA called a promoter region. 
B)  Within a given gene, usually one strand of the DNA is copied and then the other is copied into RNA. 
C)  When DNA or RNA is copied this means that from the DNA sequence GCAGTTA the copy will read GCAGTTA. 
D)  During DNA synthesis, the primer is copied into RNA but is later removed by an exonuclease. 
E)  The 5' end of a DNA molecule always has the phosphate.

Question 4

The lariat structure is a characteristic of:

Accepted Answer

A)  tRNA. 
B)  U1 SNURP. 
C)  the mRNA poly(
A)  tail. 
D)  an intron, prior to splicing. 
E)  an intron, subsequent to splicing. 
A)  tRNA. 
B)  U1 SNURP. 
C)  the mRNA poly(
A)  tail. 
D)  an intron, prior to splicing. 
E)  an intron, subsequent to splicing.

Question 5

If nucleolar DNA were isolated and studied, one would find

Accepted Answer

A)  it could be hybridized to saturation with ribosomal 18S RNA, and then would not hybridize with ribosomal 28SRNA. 
B)  it could be hybridized to saturation with ribosomal 28S RNA, and then would not hybridize with ribosomal 18SRNA. 
C)  it could be hybridized to saturation independently with both 18S and 28S RNA. 
D)  as in C, but such hybridization would show competition by messenger RNA. 
A)  it could be hybridized to saturation with ribosomal 18S RNA, and then would not hybridize with ribosomal 28SRNA. 
B)  it could be hybridized to saturation with ribosomal 28S RNA, and then would not hybridize with ribosomal 18SRNA. 
C)  it could be hybridized to saturation independently with both 18S and 28S RNA. 
D)  as in C, but such hybridization would show competition by messenger RNA.

Question 6

Which of the following statements is true about the small ribosomal subunit?

Accepted Answer

A)  It catalyzes peptide bond synthesis. 
B)  It is responsible for proofreading of the amino acid incorporated in response to a given codon. 
C)  It forms a complex with initiator tRNA and mRNA 
D)  It contains both A and P sites for tRNA attachment. 
E)  none is true 
A)  It catalyzes peptide bond synthesis. 
B)  It is responsible for proofreading of the amino acid incorporated in response to a given codon. 
C)  It forms a complex with initiator tRNA and mRNA 
D)  It contains both A and P sites for tRNA attachment. 
E)  none is true

Question 7

DNA-dependent RNA polymerase

Accepted Answer

A)  always functions as a single polypeptide subunit. 
B)  is unique to oncogenic RNA viruses. 
C)  is involved in transcription. 
D)  synthesizes DNA. 
A)  always functions as a single polypeptide subunit. 
B)  is unique to oncogenic RNA viruses. 
C)  is involved in transcription. 
D)  synthesizes DNA.

Question 8

alpha-Amanitin is toxic to eukaryotic cells because it

Accepted Answer

A)  prevents translocation of mRNA from the nucleus to the cytoplasm. 
B)  causes spliceosome assembly defects. 
C)  causes ribosome assembly defects. 
D)  causes protein transport defects. 
E)  inhibits RNA polymerase II. 
A)  prevents translocation of mRNA from the nucleus to the cytoplasm. 
B)  causes spliceosome assembly defects. 
C)  causes ribosome assembly defects. 
D)  causes protein transport defects. 
E)  inhibits RNA polymerase II.

Question 9

The beta-globin gene from a thalassemic patient was cloned and sequenced. The technician who characterized this gene informs you that a point mutation was found, but that it can not account for the thalassemia because the mutation occurred within an intron sequence. What doesn't the technician understand?

Accepted Answer

A)  splicing occurs subsequent to protein synthesis. 
B)  Introns encode general transcription factors. 
C)  Intron sequences are required for transcription termination. 
D)  Intron sequences are required for translation termination. 
E)  Certain intron sequences are absolute requirements for splicing. 
A)  splicing occurs subsequent to protein synthesis. 
B)  Introns encode general transcription factors. 
C)  Intron sequences are required for transcription termination. 
D)  Intron sequences are required for translation termination. 
E)  Certain intron sequences are absolute requirements for splicing.

Question 10

RNA polymerases and DNA polymerases have in common the property that they both

Accepted Answer

A)  require a primer for their activity. 
B)  have proofreading activity. 
C)  synthesize polynucleotides in a 5' to 3' direction. 
D)  have exonuclease activity. 
A)  require a primer for their activity. 
B)  have proofreading activity. 
C)  synthesize polynucleotides in a 5' to 3' direction. 
D)  have exonuclease activity.

Question 11

Which of the following statements about mRNA is correct?

Accepted Answer

A)  A codon near the 5' terminus of mRNA codes for the amino acid appearing at the carboxy end of a protein. 
B)  The genetic information of a mRNA is present as a non-overlapping, non-punctuated sequence of nucleotide triplets read in the 5' to direction. 
C)  Each kind of mRNA molecule can be used for protein production only by a certain kind of ribosome of a cell. 
D)  All of the possible nucleotide triplets of mRNA code for amino acids. 
E)  A given amino acid is coded for only by a single nucleotide triplet of a given mRNA. 
A)  A codon near the 5' terminus of mRNA codes for the amino acid appearing at the carboxy end of a protein. 
B)  The genetic information of a mRNA is present as a non-overlapping, non-punctuated sequence of nucleotide triplets read in the 5' to direction. 
C)  Each kind of mRNA molecule can be used for protein production only by a certain kind of ribosome of a cell. 
D)  All of the possible nucleotide triplets of mRNA code for amino acids. 
E)  A given amino acid is coded for only by a single nucleotide triplet of a given mRNA.

Question 12

Which of the following statements is true?

Accepted Answer

A)  RNA polymerase I is used to synthesize tRNA. 
B)  RNA polymerase III is used to transcribe mRNA. 
C)  RNA polymerase II is used to transcribed rRNA. 
D)  RNA polymerase binds enhancers. 
E)  Enhancers are transcription elements. 
A)  RNA polymerase I is used to synthesize tRNA. 
B)  RNA polymerase III is used to transcribe mRNA. 
C)  RNA polymerase II is used to transcribed rRNA. 
D)  RNA polymerase binds enhancers. 
E)  Enhancers are transcription elements.

Question 13

Which of the following statements concerning RNA synthesis is true?

Accepted Answer

A)  All RNA is synthesized in the nucleolus. 
B)  28S and 18S rRNA molecules are synthesized separately. 
C)  2'-O-methylribose is selectively eliminated during processing of rRNA. 
D)  Poly A is added to mRNA during processing. 
A)  All RNA is synthesized in the nucleolus. 
B)  28S and 18S rRNA molecules are synthesized separately. 
C)  2'-O-methylribose is selectively eliminated during processing of rRNA. 
D)  Poly A is added to mRNA during processing.

Question 14

All of the following statements about RNA polymerase and DNA polymerase are correct EXCEPT

Accepted Answer

A)  both require a template. 
B)  both require nucleoside triphosphates as substrates. 
C)  both elongate in the 5' to direction. 
D)  both require a primer. 
E)  both are required for replication in certain systems. 
A)  both require a template. 
B)  both require nucleoside triphosphates as substrates. 
C)  both elongate in the 5' to direction. 
D)  both require a primer. 
E)  both are required for replication in certain systems.

Question 15

Which of the following statements about the mRNAs in a typical eukaryotic cell is NOT correct?

Accepted Answer

A)  mRNA abundance is one factor that determines cellular protein levels. 
B)  Only a relatively small number of mRNA species is present at very high abundance. 
C)  Most of the mRNA complexity of a cell is in the high abundance mRNA class. 
D)  Cellular mRNA concentrations are regulated by transcriptional controls. 
A)  mRNA abundance is one factor that determines cellular protein levels. 
B)  Only a relatively small number of mRNA species is present at very high abundance. 
C)  Most of the mRNA complexity of a cell is in the high abundance mRNA class. 
D)  Cellular mRNA concentrations are regulated by transcriptional controls.

Question 16

Dimerization of transcription factor subunits accomplishes which of the following?

Accepted Answer

A)  Blocks their association with DNA 
B)  Prevents their translocation to the nucleus 
C)  Increases their binding affinity for DNA 
D)  Targets them for degradation 
A)  Blocks their association with DNA 
B)  Prevents their translocation to the nucleus 
C)  Increases their binding affinity for DNA 
D)  Targets them for degradation

Question 17

Eukaryotic cells rRNA

Accepted Answer

A)  is rapidly degraded by ribonucleases immediately after its involvement in protein synthesis. 
B)  is synthesized primarily in nucleoli. 
C)  is found in and 50S subunits of the ribosomes of the cytosol. 
D)  is coded for by at least two separate genes coding for 17S and 2precursor molecules. 
E)  has far fewer proteins associated with it than has prokaryotic rRNA. 
A)  is rapidly degraded by ribonucleases immediately after its involvement in protein synthesis. 
B)  is synthesized primarily in nucleoli. 
C)  is found in and 50S subunits of the ribosomes of the cytosol. 
D)  is coded for by at least two separate genes coding for 17S and 2precursor molecules. 
E)  has far fewer proteins associated with it than has prokaryotic rRNA.

Question 18

transcription in normal cells requires each of the following EXCEPT

Accepted Answer

A)  ribonucleoside triphosphates. 
B)  template DNA. 
C)  magnesium ions. 
D)  tRNA. 
E)  DNA-dependent RNA polymerase. 
A)  ribonucleoside triphosphates. 
B)  template DNA. 
C)  magnesium ions. 
D)  tRNA. 
E)  DNA-dependent RNA polymerase.

Question 19

Eukaryotic cells unable to synthesize small nuclear RNAs (snRNAs) would be unable to

Accepted Answer

A)  process tRNA precursors. 
B)  splice mRNA precursors. 
C)  replicate DNA. 
D)  initiate RNA synthesis at the proper sites. 
A)  process tRNA precursors. 
B)  splice mRNA precursors. 
C)  replicate DNA. 
D)  initiate RNA synthesis at the proper sites.

Question 20

The enzyme RNA polymerase synthesizes a specific RNA when provided with which of the following as a template?

Accepted Answer

A)  Soluble RNA 
B)  Ribosomal RNA 
C)  Messenger RNA 
D)  Single-stranded DNA 
E)  Double-stranded DNA 
A)  Soluble RNA 
B)  Ribosomal RNA 
C)  Messenger RNA 
D)  Single-stranded DNA 
E)  Double-stranded DNA

Exam 5: RNA: Transcription and Rna Processing

A one nucleotide slip in the splice point on the side of the splice junction in the first intron of the beta-globin gene would result in

Synthesis of the precursor-mRNA molecule of various enzymes occurs in

Which statement is true?

The lariat structure is a characteristic of:

If nucleolar DNA were isolated and studied, one would find

Which of the following statements is true about the small ribosomal subunit?

DNA-dependent RNA polymerase

alpha-Amanitin is toxic to eukaryotic cells because it

RNA polymerases and DNA polymerases have in common the property that they both

Which of the following statements about mRNA is correct?

Which of the following statements is true?

Which of the following statements concerning RNA synthesis is true?

All of the following statements about RNA polymerase and DNA polymerase are correct EXCEPT

Which of the following statements about the mRNAs in a typical eukaryotic cell is NOT correct?

Dimerization of transcription factor subunits accomplishes which of the following?

Eukaryotic cells rRNA

transcription in normal cells requires each of the following EXCEPT

Eukaryotic cells unable to synthesize small nuclear RNAs (snRNAs) would be unable to

The enzyme RNA polymerase synthesizes a specific RNA when provided with which of the following as a template?

Eukaryotic Cell Structure

DNA and RNA: Composition and Structure

Protein I: Composition and Structure

DNA Replication, Recombination, and Repair

Protein Synthesis: Translation and Posttranslational Modifications

Recombinant Dna and Biotechnology

Regulation of Gene Expression

Protein II: Structure-Function Relationships in Protein Families

Enzymes: Classification, Kinetics, and Control

The Cytochromes P450 and Nitric Oxide Synthases

Biological Membranes: Structure and Membrane Transport

Fundamentals of Signal Transduction

Bioenergetics and Oxidative Metabolism

Carbohydrate Metabolism I: Major Metabolic Pathways and Their Control

Carbohydrate Metabolism II: Special Pathways and Glycoconjugates

Lipid Metabolism I: Synthesis, Storage, and Utilization of Fatty Acids and Triacylglycerols

Lipid Metabolism II: Pathways of Metabolism of Special Lipids

Amino Acid Metabolism

Purine and Pyrimidine Nucleotide Metabolism

Metabolic Interrelationships

Biochemistry of Hormones

Molecular Cell Biology

Cell Cycle, Programmed Cell Death, and Cancer

Digestion and Absorption of Basic Nutritional Constituents

Digestion and Absorption of Basic Nutritional Constituents

Principles of Nutrition I and II

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