Exam 7: Recombinant Dna and Biotechnology

Two small plasmids undergoing recombination will look like a figure 8. If they are each cut once at a unique restriction site, they will form a Chi structure. Which of the following will be true

Which of the following would most likely be found in a recombinant plasmid containing a copy of DNA from a human gene?

Which of the following techniques can be used to screen the general population for a single base-pair mutation in disease-causing gene?

DNA sequencing makes use of

The genomic DNA library

All of the following are used to construct genomic libraries EXCEPT

According to the Holliday model, the initial steps in recombination are which of the following?

Chromosome walking

Segments of pure samples of identical, relatively short DNA molecules from plasmids can be subdivided into smaller pieces by

The "penetrance" of an inherited disease refers to the

One enzyme necessary for the genetic information of some RNA virus to be incorporated into the chromosome of a host eukaryotic cells

PCR is used for all of the following EXCEPT

Vector development, design of the corrective gene construct, proliferation and maintenance of target cells, efficient transfection and transport of nucleic acid to the nucleus for integration into the genome, and expansion of engineered cells and implantation into the patient are all examples of

Which of the following statements pertaining to genome organization is true?

Enzymes necessary for the construction of a recombinant molecule of DNA are

Yeast artificial chromosome vectors contain all of the following EXCEPT

RFLP screening during a chromosomal walk would permit the identification of a

In constructing a genomic DNA library, limited digestion with a restriction enzyme that recognizes and cuts a 4 base pair sequence

M17476PCR (Polymerase Chain Reaction) products are of discrete length because

In the Sanger dideoxy method of DNA sequencing, the dideoxy analog