Question 1

Bacteria cannot use homologous recombination for DNA repair as they only have a single copy of their genome.

Accepted Answer

A) True 
 B)False  False

Question 2

The movement of transposable genetic elements within a genome is essentially ________.

Accepted Answer

random

Question 3

O6-methylguanine has a high probability of base pairing with thymine to result in a post replication mutation from a GC base pair to:

Accepted Answer

A)  TA. 
B)  AT. 
C)  CG. 
D)  GT. 
E)  TC. 
A)  TA. 
B)  AT. 
C)  CG. 
D)  GT. 
E)  TC. B

Question 4

________ and bulky adducts on DNA can be repaired using the nucleotide excision repair process.

Accepted Answer

The answer of ________ and bulky adducts on DNA can...

Question 5

The dam methylase has a role in which of the following DNA repair systems?

Accepted Answer

A)  Base-excision repair 
B)  Nucleotide excision repair 
C)  Non-homologous end-joining 
D)  Mismatch repair 
E)  Homologous recombination 
A)  Base-excision repair 
B)  Nucleotide excision repair 
C)  Non-homologous end-joining 
D)  Mismatch repair 
E)  Homologous recombination

Question 6

The λ genome is inserted into the E. coli genome in the lysogenic pathway by the use of ________ recombination.

Accepted Answer

The answer of The λ genome is inserted into the...

Question 7

Retroviruses are characterized by the presence of ________ that flank the viral structural genes.

Accepted Answer

long termi

Question 8

The SOS response to DNA damage in bacteria is error-prone because:

Accepted Answer

A)  it uses DNA polymerase that lacks 5' exonuclease activity. 
B)  it is an inducible system. 
C)  the polymerases used lack 3' exonuclease activity. 
D)  more than one polymerase can be used to repair the damage. 
E)  it is stimulated by ultraviolet light that causes mutations. 
A)  it uses DNA polymerase that lacks 5' exonuclease activity. 
B)  it is an inducible system. 
C)  the polymerases used lack 3' exonuclease activity. 
D)  more than one polymerase can be used to repair the damage. 
E)  it is stimulated by ultraviolet light that causes mutations.

Question 9

Site-specific recombination generally requires longer homologous sequences than does homologous recombination.

Accepted Answer

A) True 
 B)False

Question 10

Transcription-coupled repair usually repairs the template strand.

Accepted Answer

A) True 
 B)False

Question 11

Base excision repair starts with cleavage of the ________ between the damaged base and the deoxyribose.

Accepted Answer

The answer of Base excision repair starts with cleavage of...

Question 12

Which of the following types of DNA damage cannot be repaired by base excision repair?

Accepted Answer

A)  Depurination 
B)  Cytosine deamination 
C)  Thymine dimer 
D)  Single-strand break 
E)  Base oxidation 
A)  Depurination 
B)  Cytosine deamination 
C)  Thymine dimer 
D)  Single-strand break 
E)  Base oxidation

Question 13

Photoreactivation using DNA photolyase is responsible for direct repair of thymine dimers in all organisms.

Accepted Answer

A) True 
 B)False

Question 14

One mechanism that could result in gene duplication involves ________ sister-chromatid exchange.

Accepted Answer

The answer of One mechanism that could result in gene...

Question 15

Strand-displacement in homologous recombination in E. coli is facilitated by:

Accepted Answer

A)  the RecBCD complex. 
B)  ss-binding protein. 
C)  RecA. 
D)  RuvC. 
E)  the chi structure. 
A)  the RecBCD complex. 
B)  ss-binding protein. 
C)  RecA. 
D)  RuvC. 
E)  the chi structure.

Question 16

Nucleotide excision repair:

Accepted Answer

A)  involves a multisubunit enzyme. 
B)  preferentially repairs genes that are being actively transcribed. 
C)  involves making nicks on either side of a DNA lesion. 
D)  uses helicase, polymerase and ligase to repair the DNA. 
E)  all of the above. 
A)  involves a multisubunit enzyme. 
B)  preferentially repairs genes that are being actively transcribed. 
C)  involves making nicks on either side of a DNA lesion. 
D)  uses helicase, polymerase and ligase to repair the DNA. 
E)  all of the above.

Question 17

The direct DNA repair enzymes DNA photolyase and O6-alkyguanine alkytransferase are unusual in that they are ________ after one catalytic cycle.

Accepted Answer

The answer of The direct DNA repair enzymes DNA photolyase...

Question 18

The only function of Uracil-DNA N-glycosylase is to remove dUMP residues in DNA that have been arisen by deamination of cytosine.

Accepted Answer

A) True 
 B)False

Question 19

Which of the following mechanisms is NOT involved in the generation of antibody diversity?

Accepted Answer

A)  Recombination with excision of DNA between a V gene sequence and a J sequence 
B)  Splicing out of RNA sequences between a J region and a C region 
C)  Somatic hypermutation 
D)  Non-homologous end joining and DNA repair 
E)  Transposition 
A)  Recombination with excision of DNA between a V gene sequence and a J sequence 
B)  Splicing out of RNA sequences between a J region and a C region 
C)  Somatic hypermutation 
D)  Non-homologous end joining and DNA repair 
E)  Transposition

Question 20

Which of the following enzyme activities is NOT used in base excision repair?

Accepted Answer

A)  Glycosylase 
B)  Apyriminidic endonuclease 
C)  Deoxyribose-5'-phosphatase 
D)  DNA polymerase 
E)  Helicase 
A)  Glycosylase 
B)  Apyriminidic endonuclease 
C)  Deoxyribose-5'-phosphatase 
D)  DNA polymerase 
E)  Helicase

Exam 23: DNA Repair, Recombination, and Rearrangement

Bacteria cannot use homologous recombination for DNA repair as they only have a single copy of their genome.

The movement of transposable genetic elements within a genome is essentially ________.

O6-methylguanine has a high probability of base pairing with thymine to result in a post replication mutation from a GC base pair to:

________ and bulky adducts on DNA can be repaired using the nucleotide excision repair process.

The dam methylase has a role in which of the following DNA repair systems?

The λ genome is inserted into the E. coli genome in the lysogenic pathway by the use of ________ recombination.

Retroviruses are characterized by the presence of ________ that flank the viral structural genes.

The SOS response to DNA damage in bacteria is error-prone because:

Site-specific recombination generally requires longer homologous sequences than does homologous recombination.

Transcription-coupled repair usually repairs the template strand.

Base excision repair starts with cleavage of the ________ between the damaged base and the deoxyribose.

Which of the following types of DNA damage cannot be repaired by base excision repair?

Photoreactivation using DNA photolyase is responsible for direct repair of thymine dimers in all organisms.

One mechanism that could result in gene duplication involves ________ sister-chromatid exchange.

Strand-displacement in homologous recombination in E. coli is facilitated by:

Nucleotide excision repair:

The direct DNA repair enzymes DNA photolyase and O6-alkyguanine alkytransferase are unusual in that they are ________ after one catalytic cycle.

The only function of Uracil-DNA N-glycosylase is to remove dUMP residues in DNA that have been arisen by deamination of cytosine.

Which of the following mechanisms is NOT involved in the generation of antibody diversity?

Which of the following enzyme activities is NOT used in base excision repair?

Biochemistry and the Language of Chemistry

The Chemical Foundation of Life: Weak Interactions in an Aqueous Environment

The Energetics of Life

Nucleic Acids

Introduction to Proteins: the Primary Level of Protein Structure

The Three-Dimensional Structure of Proteins

Protein Function and Evolution

Enzymes: Biological Catalysts

Carbohydrates: Sugars, Saccharides, Glycans

Lipids, Membranes, and Cellular Transport

Chemical Logic of Metabolism

Carbohydrate Metabolism: Glycolysis, Gluconeogenesis, Glycogen Metabolism, and the Pentose Phosphate Pathway

The Citric Acid Cycle

Electron Transport, Oxidative Phosphorylation, and Oxygen Metabolism

Photosynthesis

Lipid Metabolism

Interorgan and Intracellular Coordination of Energy Metabolism in Vertebrates

Amino Acid and Nitrogen Metabolism

Nucleotide Metabolism

Mechanisms of Signal Transduction

Genes, Genomes, and Chromosomes

DNA Replication

Transcription and Post-Transcriptional Processing

Information Decoding: Translation and Post-Translational Protein Processing

Regulation of Gene Expression

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