Question 1

In bacteria, mRNA, tRNA and rRNA are all transcribed by the same RNA polymerase.

Accepted Answer

A) True 
 B)False  True

Question 2

Which of the following is NOT correct when considering the initiation of transcription in E. coli?

Accepted Answer

A)  The σ subunit of RNA polymerase makes strong contacts with the -10 region of promoters. 
B)  Only the -10 region of the promoter is required. 
C)  DNA melting near the transcription start site is essential for promoter recognition. 
D)  The phosphodiester bonds that are formed are not stable until about 10 nucleotides have been incorporated. 
E)  The RNA polymerase contacts about 50 bp of DNA. 
A)  The σ subunit of RNA polymerase makes strong contacts with the -10 region of promoters. 
B)  Only the -10 region of the promoter is required. 
C)  DNA melting near the transcription start site is essential for promoter recognition. 
D)  The phosphodiester bonds that are formed are not stable until about 10 nucleotides have been incorporated. 
E)  The RNA polymerase contacts about 50 bp of DNA. B

Question 3

The 5' cap added to eukaryotic mRNA serves to stabilize the mRNA and assists in the efficiency of ________.

Accepted Answer

translation

Question 4

After promoter clearance by RNA polymerase II:

Accepted Answer

A)  all associated factors remain bound to the polymerase for elongation. 
B)  a subset of factors remain at the promoter region. 
C)  no further elongation factors are acquired by the polymerase. 
D)  no further chromatin remodeling is required. 
E)  transcription continues until the polymerase reaches the poly (
A)  tail. 
A)  all associated factors remain bound to the polymerase for elongation. 
B)  a subset of factors remain at the promoter region. 
C)  no further elongation factors are acquired by the polymerase. 
D)  no further chromatin remodeling is required. 
E)  transcription continues until the polymerase reaches the poly (
A)  tail.

Question 5

Degradation of bacterial mRNAs occurs:

Accepted Answer

A)  from the 3' end. 
B)  largely by the activity of non-specific nucleases. 
C)  from the 5' end. 
D)  relatively slowly. 
E)  from both 3' and 5' ends at the same time. 
A)  from the 3' end. 
B)  largely by the activity of non-specific nucleases. 
C)  from the 5' end. 
D)  relatively slowly. 
E)  from both 3' and 5' ends at the same time.

Question 6

In bacteria post-transcriptional processing of pre-rRNA species can yield mature tRNAs.

Accepted Answer

A) True 
 B)False

Question 7

In eukaryotes a set of ________ function in a comparable manner to the σ subunit of E. coli RNA polymerase.

Accepted Answer

transcript

Question 8

Most ________ of transcription in prokaryotes is based on a weakened interaction due to ________ base pairing between the template and the transcript as well as a stable stem loop structure in the nascent transcript which eliminates base pairing between the template and transcript upstream of the AU interaction.

Accepted Answer

The answer of Most ________ of transcription in prokaryotes is...

Question 9

In eukaryotes genes encoding enzymes for metabolic pathways often exist as operons of several genes with a single regulatory region to provide coordinate regulation of all genes required for the pathway.

Accepted Answer

A) True 
 B)False

Question 10

Which of the following is FALSE when comparing DNA pol III and RNA polymerase?

Accepted Answer

A)  There are many more copies of RNA polymerase in the cell. 
B)  DNA pol III is much more processive. 
C)  RNA polymerase makes more errors. 
D)  RNA polymerase has a much slower kcat. 
E)  RNA polymerase does not need a primer. 
A)  There are many more copies of RNA polymerase in the cell. 
B)  DNA pol III is much more processive. 
C)  RNA polymerase makes more errors. 
D)  RNA polymerase has a much slower kcat. 
E)  RNA polymerase does not need a primer.

Question 11

Which of the following statements about E. coli RNA polymerase is INCORRECT?

Accepted Answer

A)  It has five different subunits. 
B)  The σ subunit can be dissociated easily from the rest of the enzyme. 
C)  The σ subunit is responsible for accurate determination of transcription start sites. 
D)  Without the σ subunit the enzyme has no RNA polymerase activity. 
E)  The σ subunit has a role in regulation of transcription. 
A)  It has five different subunits. 
B)  The σ subunit can be dissociated easily from the rest of the enzyme. 
C)  The σ subunit is responsible for accurate determination of transcription start sites. 
D)  Without the σ subunit the enzyme has no RNA polymerase activity. 
E)  The σ subunit has a role in regulation of transcription.

Question 12

Rho-dependent termination of transcription makes use of a hexameric protein, which has RNA-DNA helicase activity.

Accepted Answer

A) True 
 B)False

Question 13

All the tRNA genes are transcribed by ________ in eukaryotes.

Accepted Answer

The answer of All the tRNA genes are transcribed by...

Question 14

DNA microarrays can be used for gene ________ profiling, starting with RNA and using the enzyme ________ to synthesize cDNA and simultaneously incorporate nucleotides labeled with fluorescent dyes.

Accepted Answer

expression

Question 15

Which of the following types of post-transcriptional processing does NOT occur for bacterial tRNAs?

Accepted Answer

A)  Exonuclease cleavage 
B)  Endonuclease cleavage 
C)  Non-template directed addition of bases 
D)  Methylation of bases 
E)  Addition of a phosphate group to the 5' end 
A)  Exonuclease cleavage 
B)  Endonuclease cleavage 
C)  Non-template directed addition of bases 
D)  Methylation of bases 
E)  Addition of a phosphate group to the 5' end

Question 16

Which of the following statements is FALSE?

Accepted Answer

A)  RNA polymerase II is responsible for the transcription of genes encoding protein-coding sequences only. 
B)  Many promoters recognized by RNA polymerase II contain regulatory elements several kbp upstream of the initiation site. 
C)  TFIIB has a role in converting a closed-promoter into an open-promoter complex. 
D)  The C-terminal domain of the largest subunit of RNA pol II undergoes extensive phosphorylation prior to transcription elongation. 
E)  A multiprotein complex is involved in communication between upstream control elements and proteins bound at the promoter. 
A)  RNA polymerase II is responsible for the transcription of genes encoding protein-coding sequences only. 
B)  Many promoters recognized by RNA polymerase II contain regulatory elements several kbp upstream of the initiation site. 
C)  TFIIB has a role in converting a closed-promoter into an open-promoter complex. 
D)  The C-terminal domain of the largest subunit of RNA pol II undergoes extensive phosphorylation prior to transcription elongation. 
E)  A multiprotein complex is involved in communication between upstream control elements and proteins bound at the promoter.

Question 17

Splicing of mRNA occurs in spliceosomes and chemically is a series of ________ reactions.

Accepted Answer

The answer of Splicing of mRNA occurs in spliceosomes and...

Question 18

Eukaryotic transcription normally takes place on a chromatin template at nuclease-accessible sites.

Accepted Answer

A) True 
 B)False

Question 19

Alignment of conserved sequences in promoters recognized by E. coli RNA polymerase revealed two ________ sequences upstream of the transcribed region, which are separated by similar lengths of DNA.

Accepted Answer

The answer of Alignment of conserved sequences in promoters recognized...

Question 20

RNA polymerase transcribes all DNA sequences at an equal rate.

Accepted Answer

A) True 
 B)False

Exam 24: Transcription and Post-Transcriptional Processing

In bacteria, mRNA, tRNA and rRNA are all transcribed by the same RNA polymerase.

Which of the following is NOT correct when considering the initiation of transcription in E. coli?

The 5' cap added to eukaryotic mRNA serves to stabilize the mRNA and assists in the efficiency of ________.

After promoter clearance by RNA polymerase II:

Degradation of bacterial mRNAs occurs:

In bacteria post-transcriptional processing of pre-rRNA species can yield mature tRNAs.

In eukaryotes a set of ________ function in a comparable manner to the σ subunit of E. coli RNA polymerase.

In eukaryotes genes encoding enzymes for metabolic pathways often exist as operons of several genes with a single regulatory region to provide coordinate regulation of all genes required for the pathway.

Which of the following is FALSE when comparing DNA pol III and RNA polymerase?

Which of the following statements about E. coli RNA polymerase is INCORRECT?

Rho-dependent termination of transcription makes use of a hexameric protein, which has RNA-DNA helicase activity.

All the tRNA genes are transcribed by ________ in eukaryotes.

DNA microarrays can be used for gene ________ profiling, starting with RNA and using the enzyme ________ to synthesize cDNA and simultaneously incorporate nucleotides labeled with fluorescent dyes.

Which of the following types of post-transcriptional processing does NOT occur for bacterial tRNAs?

Which of the following statements is FALSE?

Splicing of mRNA occurs in spliceosomes and chemically is a series of ________ reactions.

Eukaryotic transcription normally takes place on a chromatin template at nuclease-accessible sites.

Alignment of conserved sequences in promoters recognized by E. coli RNA polymerase revealed two ________ sequences upstream of the transcribed region, which are separated by similar lengths of DNA.

RNA polymerase transcribes all DNA sequences at an equal rate.

Biochemistry and the Language of Chemistry

The Chemical Foundation of Life: Weak Interactions in an Aqueous Environment

The Energetics of Life

Nucleic Acids

Introduction to Proteins: the Primary Level of Protein Structure

The Three-Dimensional Structure of Proteins

Protein Function and Evolution

Enzymes: Biological Catalysts

Carbohydrates: Sugars, Saccharides, Glycans

Lipids, Membranes, and Cellular Transport

Chemical Logic of Metabolism

Carbohydrate Metabolism: Glycolysis, Gluconeogenesis, Glycogen Metabolism, and the Pentose Phosphate Pathway

The Citric Acid Cycle

Electron Transport, Oxidative Phosphorylation, and Oxygen Metabolism

Photosynthesis

Lipid Metabolism

Interorgan and Intracellular Coordination of Energy Metabolism in Vertebrates

Amino Acid and Nitrogen Metabolism

Nucleotide Metabolism

Mechanisms of Signal Transduction

Genes, Genomes, and Chromosomes

DNA Replication

DNA Repair, Recombination, and Rearrangement

Information Decoding: Translation and Post-Translational Protein Processing

Regulation of Gene Expression

Filters

DNA microarrays can be used for gene profiling, starting with RNA and using the enzyme to synthesize cDNA and simultaneously incorporate nucleotides labeled with fluorescent dyes.