Exam 21: Genes, Genomes, and Chromosomes

If 10.4 bp of DNA represents a linear length of 3.4 nm, how long (in meters) would one copy of the human genome (~3 × 109 bp) be if all of the DNA was placed end to end in a linear fashion?

Approximately how much of the human genome has a biological function?

The post-translational modifications of histone proteins have functional roles in gene expression.

Calculate the number of times a recognition site for a 4 bp restriction endonuclease is likely to occur in the diploid human genome

The repeating unit of chromatin is known as the ________.

Random variations among human genome sequences can result in gain or loss of ________ endonuclease cleavage sites.

If a PCR reaction starts out with 20 double-stranded DNA targets, how many specific PCR products could be formed after 35 cycles of denaturation, annealing and extension.

Restriction-modification systems:

The ________ activity associated with restriction-modification systems is able to flip out the target base from the DNA.

Nucleosomal core particles contain ________ bp of DNA wrapped around a histone octamer.

Heterochromatin is transcriptionally active.

A bacterial genome is packaged into a nucleoid, which is organized mainly by:

Southern blotting and hybridization is a technique that uses specific probes to analyze:

The human genome contains one gene for every protein that can be translated from the mRNA.

In eukaryotic cells, chromosomes are organized into a DNA-protein complex known as chromatin.

The linker histone H1 has a high proportion of the following amino acid residue.