Exam 17: Applications of Recombinant Dna Technology and Reverse Genetics

In 1978, Arber, Smith, and Nathans won the Nobel Prize in Physiology and Medicine for work with restriction enzymes. Which of the following can still be performed without using these enzymes?

When a transgene is used in mice to knock out a specific function, two problems that might occur are the unintended introduction of multiple copies or abnormal gene expression. The second of these problems is related to __________.

By what method is T-DNA integrated into the plant genome?

Which of the following vectors would be most efficient to use to amplify fragments ~300 kb in length?

In the pUC plasmids, a polylinker region that includes multiple restriction sites is embedded in the lacZ component. If a sequence of interest is inserted into this region, what is the effect on lacZ?

Specific sequences in the $\lambda$ genome that interact with coat proteins to package genomes into phage particles are known as ________ sites.

In nature, what is the order of events in processing the product of the gene for insulin?

If a researcher is cloning a sequence by using pUC and uses the plasmid to transform bacteria, which are then plated on X-gal medium, would the recombinant plasmids be found in blue or white colonies or both?