Question 1

What are the three mechanisms by which chromatin remodelers can move nucleosomes?

Accepted Answer

nucleosome sliding,

Question 2

Regulatory regions of a eukaryotic gene all contain which of the following sequences, which regulate transcription of genes located on the same chromosome as the sequences?

Accepted Answer

A) zinc fingers 
B) cis-acting regulatory sequences 
C) homeodomains 
D) trans-acting regulatory sequences 
E) leucine zippers 
A) zinc fingers 
B) cis-acting regulatory sequences 
C) homeodomains 
D) trans-acting regulatory sequences 
E) leucine zippers

Question 3

________ in Drosophila results from the movement of the transcriptionally active w+ allele into the centromeric heterochromatin region of the fruit-fly X chromosome.

Accepted Answer

Position e

Question 4

Many types of cancer are known to overexpress the receptor protein tyrosine kinase. Which molecular technique can be used to reduce expression of an oncogene in vitro?

Accepted Answer

A) PCR 
B) DNAse sensitivity assay 
C) RNAi 
D) Southern blotting 
E) western blotting 
A) PCR 
B) DNAse sensitivity assay 
C) RNAi 
D) Southern blotting 
E) western blotting

Question 5

For the following gene, MspI and HpaII restriction sites and their positions are indicated here. Recall that HpaII is methylation sensitive, while MspI is not.   If this gene is methylated, which of the following combinations of DNA fragments would you expect from this restriction digest?

Accepted Answer

A) 5 bands = 100 bp, 200 bp, 1000 bp, 1500 bp, and 1800 bp. 
B) 4 bands = 100 bp, 200 bp, 800 bp, and 900 bp. 
C) 3 bands = 200 bp, 500 bp, and 1300 bp. 
D) 1 band = 2000 bp. 
E) No bands = DNA will be completely digested. 
A) 5 bands = 100 bp, 200 bp, 1000 bp, 1500 bp, and 1800 bp. 
B) 4 bands = 100 bp, 200 bp, 800 bp, and 900 bp. 
C) 3 bands = 200 bp, 500 bp, and 1300 bp. 
D) 1 band = 2000 bp. 
E) No bands = DNA will be completely digested.

Question 6

Explain what effect histone deacetylase inhibitors would have on gene expression and why they may be an effective anticancer therapeutic because of their impact on tumor suppressor genes.

Accepted Answer

Histone acetylation interferes with the

Question 7

Tortoiseshell cats have a coat-color gene on their X chromosome. What process in eukaryotes explains why males can have solid orange or black fur, and only females can have the tortoiseshell pattern of fur?

Accepted Answer

The gene encoding coat color is X-linked

Question 8

Gal4 binding leads to the formation of a multiprotein complex known as the ________, which is an enhanceosome that forms after the activator protein binds.

Accepted Answer

The answer of Gal4 binding leads to the formation of...

Question 9

Galactose is absent, but you find active transcription of the GAL genes. Which protein is likely mutated or absent to allow for constitutive transcription of the GAL genes?

Accepted Answer

A) Gal2 
B) Mig1 
C) Gal4 
D) Gal10 
E) Gal80 
A) Gal2 
B) Mig1 
C) Gal4 
D) Gal10 
E) Gal80

Question 10

Inherited mutations in the Xist promoter lead to defects in which epigenetic process?

Accepted Answer

A) RNAi 
B) X-inactivation 
C) genomic imprinting 
D) position effect variegation 
E) methylation of CpG islands 
A) RNAi 
B) X-inactivation 
C) genomic imprinting 
D) position effect variegation 
E) methylation of CpG islands

Question 11

If you want to affect chromatin packaging, which amino acid could you mutate to affect both histone acetylation and methylation patterns?

Accepted Answer

A) arginine 
B) methionine 
C) histidine 
D) lysine 
E) asparagine 
A) arginine 
B) methionine 
C) histidine 
D) lysine 
E) asparagine

Question 12

During RNAi, what do miRNAs target for destruction?

Accepted Answer

A) heterochromatic regions of DNA 
B) CpG islands 
C) mRNAs 
D) histones 
E) ribosomes 
A) heterochromatic regions of DNA 
B) CpG islands 
C) mRNAs 
D) histones 
E) ribosomes

Question 13

Molecular biologists can determine experimentally whether a region of DNA contains closed chromatin or open chromatin by assessing the sensitivity of the region to __________.

Accepted Answer

A) nucleosomes 
B) histone deacetylase 
C) RNA polymerase II 
D) methyltransferase 
E) DNAse 
A) nucleosomes 
B) histone deacetylase 
C) RNA polymerase II 
D) methyltransferase 
E) DNAse

Question 14

For the following gene, which type of regulatory sequence has likely been deleted in mutant 1?
Mutant # Deletion Region % Transcription
WT None 100%
1 1-200 150%
2 250-400 100%
3 500-800 50%
4 950-1100 0%

Accepted Answer

The answer of For the following gene, which type of...

Question 15

Gal80 is mutated such that it cannot interact with Gal3. What effect would you expect to see in the presence of galactose?

Accepted Answer

Transcript

Question 16

When CpG islands are unmethylated, __________.

Accepted Answer

A) chromatin in the promoter region is open, allowing access by transcription factors and RNA polymerase 
B) chromatin in the promoter region is closed, preventing transcription factors and RNA polymerase from binding 
C) chromatin in the enhancer region is closed, so they are unable to bind regulatory proteins to initiate transcription 
D) DNAse hypersensitivity in that region of the chromosome is lost 
E) genes downstream of the CpG islands cannot be expressed, because the promoter region is blocked by histones 
A) chromatin in the promoter region is open, allowing access by transcription factors and RNA polymerase 
B) chromatin in the promoter region is closed, preventing transcription factors and RNA polymerase from binding 
C) chromatin in the enhancer region is closed, so they are unable to bind regulatory proteins to initiate transcription 
D) DNAse hypersensitivity in that region of the chromosome is lost 
E) genes downstream of the CpG islands cannot be expressed, because the promoter region is blocked by histones

What are the three mechanisms by which chromatin remodelers can move nucleosomes?

Regulatory regions of a eukaryotic gene all contain which of the following sequences, which regulate transcription of genes located on the same chromosome as the sequences?

________ in Drosophila results from the movement of the transcriptionally active w⁺ allele into the centromeric heterochromatin region of the fruit-fly X chromosome.

Many types of cancer are known to overexpress the receptor protein tyrosine kinase. Which molecular technique can be used to reduce expression of an oncogene in vitro?

For the following gene, MspI and HpaII restriction sites and their positions are indicated here. Recall that HpaII is methylation sensitive, while MspI is not. If this gene is methylated, which of the following combinations of DNA fragments would you expect from this restriction digest?

Explain what effect histone deacetylase inhibitors would have on gene expression and why they may be an effective anticancer therapeutic because of their impact on tumor suppressor genes.

Tortoiseshell cats have a coat-color gene on their X chromosome. What process in eukaryotes explains why males can have solid orange or black fur, and only females can have the tortoiseshell pattern of fur?

Gal4 binding leads to the formation of a multiprotein complex known as the ________, which is an enhanceosome that forms after the activator protein binds.

Galactose is absent, but you find active transcription of the GAL genes. Which protein is likely mutated or absent to allow for constitutive transcription of the GAL genes?

Inherited mutations in the Xist promoter lead to defects in which epigenetic process?

If you want to affect chromatin packaging, which amino acid could you mutate to affect both histone acetylation and methylation patterns?

During RNAi, what do miRNAs target for destruction?

Molecular biologists can determine experimentally whether a region of DNA contains closed chromatin or open chromatin by assessing the sensitivity of the region to __________.

For the following gene, which type of regulatory sequence has likely been deleted in mutant 1? Mutant # Deletion Region % Transcription WT None 100% 1 1-200 150% 2 250-400 100% 3 500-800 50% 4 950-1100 0%

Gal80 is mutated such that it cannot interact with Gal3. What effect would you expect to see in the presence of galactose?

When CpG islands are unmethylated, __________.

The Molecular Basis of Heredity, Variation, and Evolution

Transmission Genetics

Cell Division and Chromosome Heredity

Gene Interaction

Genetic Linkage and Mapping in Eukaryotes

Genetic Analysis and Mapping in Bacteria and Bacteriophages

Dna Structure and Replication

Molecular Biology and Transcription and Rna Processing

The Molecular Biology of Translation

The Integration of Genetic Approaches: Understanding Sickle Cell Disease

Chromosome Structure

Gene Mutation, Dna Repair, and Homologous Recombination

Chromosome Aberrations and Transposition

Regulation of Gene Expression in Bacteria and Bacteriophages

Forward Genetics and Recombinant Dna Technology

Applications of Recombinant Dna Technology and Reverse Genetics

Genomics: Genetics From a Whole-Genome Perspective

Cytoplasmic Inheritance and the Evolution of Organelle Genomes

Developmental Genetics

Genetic Analysis of Quantitative Traits

Population Genetics and Evolution

Filters

Exam 15: Regulation of Gene Expression in Eukaryotes

What are the three mechanisms by which chromatin remodelers can move nucleosomes?

Regulatory regions of a eukaryotic gene all contain which of the following sequences, which regulate transcription of genes located on the same chromosome as the sequences?

________ in Drosophila results from the movement of the transcriptionally active w+ allele into the centromeric heterochromatin region of the fruit-fly X chromosome.

Many types of cancer are known to overexpress the receptor protein tyrosine kinase. Which molecular technique can be used to reduce expression of an oncogene in vitro?

For the following gene, MspI and HpaII restriction sites and their positions are indicated here. Recall that HpaII is methylation sensitive, while MspI is not. If this gene is methylated, which of the following combinations of DNA fragments would you expect from this restriction digest?

Explain what effect histone deacetylase inhibitors would have on gene expression and why they may be an effective anticancer therapeutic because of their impact on tumor suppressor genes.

Tortoiseshell cats have a coat-color gene on their X chromosome. What process in eukaryotes explains why males can have solid orange or black fur, and only females can have the tortoiseshell pattern of fur?

Gal4 binding leads to the formation of a multiprotein complex known as the ________, which is an enhanceosome that forms after the activator protein binds.

Galactose is absent, but you find active transcription of the GAL genes. Which protein is likely mutated or absent to allow for constitutive transcription of the GAL genes?

Inherited mutations in the Xist promoter lead to defects in which epigenetic process?

If you want to affect chromatin packaging, which amino acid could you mutate to affect both histone acetylation and methylation patterns?

During RNAi, what do miRNAs target for destruction?

Molecular biologists can determine experimentally whether a region of DNA contains closed chromatin or open chromatin by assessing the sensitivity of the region to __________.

For the following gene, which type of regulatory sequence has likely been deleted in mutant 1? Mutant # Deletion Region % Transcription WT None 100% 1 1-200 150% 2 250-400 100% 3 500-800 50% 4 950-1100 0%

Gal80 is mutated such that it cannot interact with Gal3. What effect would you expect to see in the presence of galactose?

When CpG islands are unmethylated, __________.

The Molecular Basis of Heredity, Variation, and Evolution

Transmission Genetics

Cell Division and Chromosome Heredity

Gene Interaction

Genetic Linkage and Mapping in Eukaryotes

Genetic Analysis and Mapping in Bacteria and Bacteriophages

Dna Structure and Replication

Molecular Biology and Transcription and Rna Processing

The Molecular Biology of Translation

The Integration of Genetic Approaches: Understanding Sickle Cell Disease

Chromosome Structure

Gene Mutation, Dna Repair, and Homologous Recombination

Chromosome Aberrations and Transposition

Regulation of Gene Expression in Bacteria and Bacteriophages

Forward Genetics and Recombinant Dna Technology

Applications of Recombinant Dna Technology and Reverse Genetics

Genomics: Genetics From a Whole-Genome Perspective

Cytoplasmic Inheritance and the Evolution of Organelle Genomes

Developmental Genetics

Genetic Analysis of Quantitative Traits

Population Genetics and Evolution

Filters

________ in Drosophila results from the movement of the transcriptionally active w⁺ allele into the centromeric heterochromatin region of the fruit-fly X chromosome.