Question 1

The enzymes that deal with toxic oxygen-containing molecules is/are

Accepted Answer

A) glycolase. 
B) superoxide dismutase. 
C) catalase. 
D) hydrogen peroxidase. 
E) superoxide dismutase AND catalase. 
A) glycolase. 
B) superoxide dismutase. 
C) catalase. 
D) hydrogen peroxidase. 
E) superoxide dismutase AND catalase. E

Question 2

When doing experiments with bacteria

Accepted Answer

A) it is usually not necessary to standardize which stage of growth is used. 
B) it is best to use colonies as all the bacteria in a colony are at the same stage of growth. 
C) it is best to use bacteria from the same stage of growth. 
D) the age of the bacteria is not important. 
A) it is usually not necessary to standardize which stage of growth is used. 
B) it is best to use colonies as all the bacteria in a colony are at the same stage of growth. 
C) it is best to use bacteria from the same stage of growth. 
D) the age of the bacteria is not important. C

Question 3

Late log phase of the bacterial growth curve

Accepted Answer

A) is marked by the production of primary metabolites. 
B) is marked by the production of secondary metabolites. 
C) is a transition into the death phase. 
D) shows a decline in cell numbers. 
A) is marked by the production of primary metabolites. 
B) is marked by the production of secondary metabolites. 
C) is a transition into the death phase. 
D) shows a decline in cell numbers. B

Question 4

In nature, bacteria

Accepted Answer

A) often grow in close association with many other kinds of organisms. 
B) may remain in a prolonged exponential phase. 
C) frequently synthesize structures such as slime layers. 
D) may adhere to surfaces by means of pili and slime layers. 
E) All of the choices are correct. 
A) often grow in close association with many other kinds of organisms. 
B) may remain in a prolonged exponential phase. 
C) frequently synthesize structures such as slime layers. 
D) may adhere to surfaces by means of pili and slime layers. 
E) All of the choices are correct.

Question 5

Which of the following is/are obligate aerobes?

Accepted Answer

A) Clostridium botulinum 
B) Escherichia coli 
C) Micrococcus luteus 
D) Helicobacter pylori 
A) Clostridium botulinum 
B) Escherichia coli 
C) Micrococcus luteus 
D) Helicobacter pylori

Question 6

Carbon, hydrogen, oxygen, nitrogen, phosphorus, and sulfur may be considered

Accepted Answer

A) minor elements. 
B) major elements. 
C) neutral elements. 
D) acidic elements. 
A) minor elements. 
B) major elements. 
C) neutral elements. 
D) acidic elements.

Question 7

An organism called Bacillus fastidiosus

Accepted Answer

A) might be expected to be very flexible as to growth requirements. 
B) might be expected to be very strict as to its growth requirements. 
C) would probably be grown on a chemically defined media. 
D) might be expected to have a rod shape. 
E) might be expected to be very strict as to its growth requirements, would probably be grown on a chemically defined media AND might be expected to have a rod shape. 
A) might be expected to be very flexible as to growth requirements. 
B) might be expected to be very strict as to its growth requirements. 
C) would probably be grown on a chemically defined media. 
D) might be expected to have a rod shape. 
E) might be expected to be very strict as to its growth requirements, would probably be grown on a chemically defined media AND might be expected to have a rod shape.

Question 8

Peptone

Accepted Answer

A) refers to a hydrolysate of carbohydrates used in growth media. 
B) refers to a hydrolysate of proteins used in growth media. 
C) consists of a water extract of beef. 
D) consists of a mix of monosaccharides and oligosaccharides. 
E) refers to a hydrolysate of proteins used in growth media AND consists of a water extract of beef. 
A) refers to a hydrolysate of carbohydrates used in growth media. 
B) refers to a hydrolysate of proteins used in growth media. 
C) consists of a water extract of beef. 
D) consists of a mix of monosaccharides and oligosaccharides. 
E) refers to a hydrolysate of proteins used in growth media AND consists of a water extract of beef.

Question 9

The scientist that contributed most to the development of pure culture techniques was

Accepted Answer

A) Alexander Fleming. 
B) Louis Pasteur. 
C) Edward Jenner. 
D) Robert Koch. 
A) Alexander Fleming. 
B) Louis Pasteur. 
C) Edward Jenner. 
D) Robert Koch.

Question 10

A single bacterial cell may multiply to form a visible colony.

Accepted Answer

A) True 
 B)False

Question 11

Bacteria may be stored

Accepted Answer

A) on a slant in the refrigerator. 
B) frozen in glycerol solution. 
C) lyophilized. 
D) in broth at 37°C. 
E) on a slant in the refrigerator, frozen in glycerol solution AND lyophilized. 
A) on a slant in the refrigerator. 
B) frozen in glycerol solution. 
C) lyophilized. 
D) in broth at 37°C. 
E) on a slant in the refrigerator, frozen in glycerol solution AND lyophilized.

Question 12

Differential media only allows certain bacteria to grow.

Accepted Answer

A) True 
 B)False

Question 13

Trace elements

Accepted Answer

A) include zinc, copper and manganese. 
B) are required in large amounts. 
C) may be needed for enzyme function. 
D) are involved in maintaining pH in the cell. 
E) include zinc, copper and manganese AND may be needed for enzyme function. 
A) include zinc, copper and manganese. 
B) are required in large amounts. 
C) may be needed for enzyme function. 
D) are involved in maintaining pH in the cell. 
E) include zinc, copper and manganese AND may be needed for enzyme function.

Question 14

Agar

Accepted Answer

A) is a useful nutrient source for most bacteria. 
B) is a hydrolysate of proteins. 
C) stays liquid through the typical range of incubation temperatures. 
D) has chemical and physical properties that make it almost ideal for solidifying media. 
A) is a useful nutrient source for most bacteria. 
B) is a hydrolysate of proteins. 
C) stays liquid through the typical range of incubation temperatures. 
D) has chemical and physical properties that make it almost ideal for solidifying media.

Question 15

During which phase of growth are bacteria most susceptible to antibiotics?

Accepted Answer

A) lag 
B) stationary 
C) exponential (log) 
D) decline 
A) lag 
B) stationary 
C) exponential (log) 
D) decline

Question 16

In microbiology, growth usually refers to an increase in size of the bacteria.

Accepted Answer

A) True 
 B)False

Question 17

Candle jars are usually used to

Accepted Answer

A) store candles. 
B) provide an atmosphere with CO2. 
C) stimulate the growth of obligate anaerobes. 
D) prevent the growth of obligate aerobes. 
E) provide an atmosphere with CO2 AND stimulate the growth of obligate anaerobes. 
A) store candles. 
B) provide an atmosphere with CO2. 
C) stimulate the growth of obligate anaerobes. 
D) prevent the growth of obligate aerobes. 
E) provide an atmosphere with CO2 AND stimulate the growth of obligate anaerobes.

Question 18

You are working in a clinical laboratory in a hospital setting. You're handed a throat swab from a patient. You are told specifically that the physician is only interested in what Gram positive cells are present. What might you do first to go about working towards this goal? Identification isn't the main goal here-just a first step to work towards determining what Gram positive cells might be there.

Accepted Answer

A) Perform a Gram stain. 
B) Streak the sample for isolation on a tryptic soy agar general purpose medium plate. 
C) Streak the sample for isolation on a medium that is selective for Gram positive cells while suppressing Gram negative cell growth. 
D) Grow the microbes on the swab by inoculating a tryptic soy broth liquid medium tube. 
E) Perform an acid-fast stain. 
A) Perform a Gram stain. 
B) Streak the sample for isolation on a tryptic soy agar general purpose medium plate. 
C) Streak the sample for isolation on a medium that is selective for Gram positive cells while suppressing Gram negative cell growth. 
D) Grow the microbes on the swab by inoculating a tryptic soy broth liquid medium tube. 
E) Perform an acid-fast stain.

Question 19

MacConkey agar is

Accepted Answer

A) a selective agar. 
B) a differential agar. 
C) a selective and differential agar. 
D) used to distinguish between bacteria by the type of hemolysis observed. 
A) a selective agar. 
B) a differential agar. 
C) a selective and differential agar. 
D) used to distinguish between bacteria by the type of hemolysis observed.

Question 20

The speed of enzymatic reactions approximately doubles for each

Accepted Answer

A) 5° C rise in temperature. 
B) 10° C rise in temperature. 
C) 20° C rise in temperature. 
D) 30° C rise in temperature. 
A) 5° C rise in temperature. 
B) 10° C rise in temperature. 
C) 20° C rise in temperature. 
D) 30° C rise in temperature.

Exam 4: Dynamics of Prokaryotic Growth

The enzymes that deal with toxic oxygen-containing molecules is/are

When doing experiments with bacteria

Late log phase of the bacterial growth curve

In nature, bacteria

Which of the following is/are obligate aerobes?

Carbon, hydrogen, oxygen, nitrogen, phosphorus, and sulfur may be considered

An organism called Bacillus fastidiosus

Peptone

The scientist that contributed most to the development of pure culture techniques was

A single bacterial cell may multiply to form a visible colony.

Bacteria may be stored

Differential media only allows certain bacteria to grow.

Trace elements

Agar

During which phase of growth are bacteria most susceptible to antibiotics?

In microbiology, growth usually refers to an increase in size of the bacteria.

Candle jars are usually used to

MacConkey agar is

The speed of enzymatic reactions approximately doubles for each

Humans and the Microbial World

The Molecules of Life

Microscopy and Cell Structure

Control of Microbial Growth

Metabolism: Fueling Cell Growth

The Blueprint of Life, From Dna to Protein

Bacterial Genetics

Biotechnology and Recombinant Dna

Identification and Classification of Prokaryotes

The Diversity of Prokaryotic Organisms

The Eukaryotic Members of the Microbial World

Viruses, Viroids and Prions

The Innate Immune Response

The Adaptive Immune Response

Host-Microbe Interactions

Immunologic Disorders

Applications of Immune Responses

Epidemiology

Antimicrobial Medications

Respiratory System Infections

Skin Infections

Wound Infections

Digestive System Infections

Genitourinary Infections

Nervous System Infections

Blood and Lymphatic Infections

Hiv Disease and Complications of Immunodeficiency

Microbial Ecology

Environmental Microbiology: Treatment of Water, Wastes, and Polluted Habitats

Food Microbiology

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