Question 1

In general,the main goal of cloning is to include as many different genes as possible in a single cloning vector.

Accepted Answer

A) True 
 B)False

Question 2

Of what advantage is it to have a multiple cloning site (multiple unique restriction sites)embedded in the lacZ component of a plasmid?

Accepted Answer

An insert of DNA in the multip

Question 3

Restriction endonucleases typically recognize palindromic DNA sequences and often generate "sticky ends" or single-stranded DNA overhangs at cut sites.

Accepted Answer

A) True 
 B)False

Question 4

Vectors such as pUC18 and others of the pUC series contain a large number of restriction enzyme sites clustered in one region.Which term describes this advantageous arrangement of restriction sites?

Accepted Answer

A) palindrome 
B) consensus sequence 
C) multiple cloning site 
D) $\beta$-galactosidase 
E) complementation 
A) palindrome 
B) consensus sequence 
C) multiple cloning site 
D) $\beta$-galactosidase 
E) complementation

Question 5

In the context of molecular genetics,reverse transcription PCR (RT-PCR)refers to ________.

Accepted Answer

A) assembling a DNA sequence from an mRNA 
B) assembling an RNA sequence from a DNA sequence 
C) translating in the 3' to 5' direction 
D) transcribing first,then translating 
E) making an amino acid sequence from a DNA sequence 
A) assembling a DNA sequence from an mRNA 
B) assembling an RNA sequence from a DNA sequence 
C) translating in the 3' to 5' direction 
D) transcribing first,then translating 
E) making an amino acid sequence from a DNA sequence

Question 6

List two especially useful characteristics of cloning vectors.

Accepted Answer

A) high copy number and antibiotic resistance gene(s) 
B) virulence and lysogenicity 
C) ability to integrate into the host chromosome and then causing a lytic cycle 
D) nonautonomous replication and transposition 
E) reverse transcriptase and ligase activities 
A) high copy number and antibiotic resistance gene(s) 
B) virulence and lysogenicity 
C) ability to integrate into the host chromosome and then causing a lytic cycle 
D) nonautonomous replication and transposition 
E) reverse transcriptase and ligase activities

Question 7

Nucleic acid blotting is commonly used in molecular biology.Two types,Southern blots and Northern blots,involve gel electrophoresis and a filter,which holds the nucleic acid.Briefly describe the procedure of "blotting" in this context and differentiate between Southern and Northern blots.

Accepted Answer

In a Southern blot,the DNA to be "probed

Question 8

What is recombinant DNA technology? What are the safety issues related to recombinant DNA technology?

Accepted Answer

Recombinant DNA technology refers to the

Question 9

The function of a ddNTP in DNA sequencing is to methylate guanine.

Accepted Answer

A) True 
 B)False

Question 10

One of the primary reasons for generating a large number of clones in a eukaryotic genomic library is that ________.

Accepted Answer

A) each cosmid replicates in coordination with the host chromosome 
B) lysogenic phages continue to integrate their DNA into the host chromosome,thus reducing the number of desired recombinant clones 
C) each vector can take up only a relatively small fraction of the eukaryotic DNA 
D) each ligation product is sequence specific 
E) the host range of the vector is limited 
A) each cosmid replicates in coordination with the host chromosome 
B) lysogenic phages continue to integrate their DNA into the host chromosome,thus reducing the number of desired recombinant clones 
C) each vector can take up only a relatively small fraction of the eukaryotic DNA 
D) each ligation product is sequence specific 
E) the host range of the vector is limited

Question 11

Restriction endonucleases are especially useful if they generate "sticky" ends.What makes an end sticky?

Accepted Answer

A) single-stranded complementary tails 
B) blunt ends 
C) poly-A sequences 
D) 5' cap 
E) interference 
A) single-stranded complementary tails 
B) blunt ends 
C) poly-A sequences 
D) 5' cap 
E) interference

Question 12

Reverse transcriptase is often used as the heat-stable enzyme in PCR.

Accepted Answer

A) True 
 B)False

Question 13

Assume that a plasmid (circular)is 3200 base pairs in length and has restriction sites at the following locations: 400,700,1400,2600.Give the expected sizes of the restriction fragments following complete digestion.

Accepted Answer

A) 400,800,1000 (2 of these) 
B) 400,1200,1600 
C) 300,700,2200 
D) 700,400,1400,2600 
E) 300,700,1000,1200 
A) 400,800,1000 (2 of these) 
B) 400,1200,1600 
C) 300,700,2200 
D) 700,400,1400,2600 
E) 300,700,1000,1200

Question 14

A ddNTP,used often in DNA sequencing,lacks a(n)________ at the ________ and ________ carbons.

Accepted Answer

A) OH; 2'; 3' 
B) methyl; 2'; 3' 
C) carboxyl; 5'; 3' 
D) OH; 2'; 5' 
E) None of the listed answers is correct. 
A) OH; 2'; 3' 
B) methyl; 2'; 3' 
C) carboxyl; 5'; 3' 
D) OH; 2'; 5' 
E) None of the listed answers is correct.

Question 15

Following are four processes common to most cloning experiments:
$\quad$transforming bacteria
$\quad$plating bacteria on selective medium
$\quad$cutting DNA with restriction endonucleases
$\quad$ligating DNA fragments
Place these components in the order in which they would most likely occur during a cloning experiment.

Accepted Answer

cutting DNA with restriction e

Question 16

What is the name of the process by which bacterial colonies (cells)are transferred from one agar plate to another,maintaining the same spatial pattern?

Accepted Answer

The answer of What is the name of the process...

Question 17

Some restriction endonucleases are capable of producing blunt ends; others can generate "sticky" ends.

Accepted Answer

A) True 
 B)False

Question 18

To isolate a bacterium with a plasmid that carries a desired DNA fragment cloned within the ampicillin resistance gene,we should grow bacteria in a medium that contains ampicillin.

Accepted Answer

A) True 
 B)False

Question 19

In the context of recombinant DNA technology,what is meant by the term vector?

Accepted Answer

A vector is a vehicle to carry

Question 20

In which of the following biochemical reactions is it common to use ddNTPs (dideoxyribonucleoside triphosphates)?

Accepted Answer

A) citric acid cycle 
B) DNA sequencing 
C) restriction digestion 
D) electron transport 
E) plasmolysis 
A) citric acid cycle 
B) DNA sequencing 
C) restriction digestion 
D) electron transport 
E) plasmolysis

Exam 17: Recombinant DNA Technology

In general,the main goal of cloning is to include as many different genes as possible in a single cloning vector.

Of what advantage is it to have a multiple cloning site (multiple unique restriction sites)embedded in the lacZ component of a plasmid?

Restriction endonucleases typically recognize palindromic DNA sequences and often generate "sticky ends" or single-stranded DNA overhangs at cut sites.

Vectors such as pUC18 and others of the pUC series contain a large number of restriction enzyme sites clustered in one region.Which term describes this advantageous arrangement of restriction sites?

In the context of molecular genetics,reverse transcription PCR (RT-PCR)refers to ________.

List two especially useful characteristics of cloning vectors.

Nucleic acid blotting is commonly used in molecular biology.Two types,Southern blots and Northern blots,involve gel electrophoresis and a filter,which holds the nucleic acid.Briefly describe the procedure of "blotting" in this context and differentiate between Southern and Northern blots.

What is recombinant DNA technology? What are the safety issues related to recombinant DNA technology?

The function of a ddNTP in DNA sequencing is to methylate guanine.

One of the primary reasons for generating a large number of clones in a eukaryotic genomic library is that ________.

Restriction endonucleases are especially useful if they generate "sticky" ends.What makes an end sticky?

Reverse transcriptase is often used as the heat-stable enzyme in PCR.

Assume that a plasmid (circular)is 3200 base pairs in length and has restriction sites at the following locations: 400,700,1400,2600.Give the expected sizes of the restriction fragments following complete digestion.

A ddNTP,used often in DNA sequencing,lacks a(n)________ at the ________ and ________ carbons.

What is the name of the process by which bacterial colonies (cells)are transferred from one agar plate to another,maintaining the same spatial pattern?

Some restriction endonucleases are capable of producing blunt ends; others can generate "sticky" ends.

To isolate a bacterium with a plasmid that carries a desired DNA fragment cloned within the ampicillin resistance gene,we should grow bacteria in a medium that contains ampicillin.

In the context of recombinant DNA technology,what is meant by the term vector?

In which of the following biochemical reactions is it common to use ddNTPs (dideoxyribonucleoside triphosphates)?

Introduction to Genetics

Mitosis and Meiosis

Mendelian Genetics

Modification of Mendelian Ratios

Sex Determination and Sex Chromosomes

Chromosome Mutations: Variation in Number and Arrangement

Linkage and Chromosome Mapping in Eukaryotes

Genetic Analysis and Mapping in Bacteria and Bacteriophages

DNA structure and Analysis

DNA Replication and Recombination

Chromosome Structure and DNA Sequence Organization

The Genetic Code and Transcription

Translation and Proteins

Gene Mutation,DNA Repair,and Transposition

Regulation of Gene Expression

The Genetics of Cancer

Genomics,Bioinformatics,and Proteomics

Applications and Ethics of Genetic Engineering and Biotechnology

Developmental Genetics

Quantitative Genetics and Multifactorial Traits

Population and Evolutionary Genetics

Conservation Genetics

Filters

A ddNTP,used often in DNA sequencing,lacks a(n) at the and carbons.