Exam 5: Methods in Protein Biochemistry

The figure below shows how differential centrifugation can be used to isolate four subcellular fractions. Which fraction corresponds to the cytosol fraction?

How can the mass of an unknown protein can be determined using gel electrophoresis?

How does the directionality of synthesis differ with in vivo polypeptide synthesis compared to solid-phase peptide synthesis?

When preparing to isolate proteins from plant cells, the first step in preparing the cell homogenate would be

During the production of polyclonal antibodies, where are the antibodies isolated from?

In an ELISA, the capture antibody is a antibody.

Why is protein purification often referred to as an art and a science?

Calculate the purification of the target protein when there is a 30% decrease in activity and a 55% decrease in total protein after centrifugation.

In isoelectric focusing, a protein with a pH below the pI would

After centrifugation, the purity of the protein is determined by

What is the advantage of using monoclonal antibodies compared with polyclonal antibodies?

Explain how protein samples that contain the antigenic protein are recognized using a Western blot.

Why is X-ray crystallography more commonly used than NMR spectroscopy when determining protein structure?

Describe how an ELISA detects antigenic proteins.

Which type of chromatography separates proteins using specific binding properties?

How does NMR spectroscopy differ from X-ray crystallography when determining the structure of a protein?

In monoclonal antibody generation, the cells that produce the antibody in the animal are located in cells.

Which technique uses highly specific monoclonal antibodies in a high-throughput form to detect small amounts of antigen?

What is the advantage of using polyclonal antibodies compared with monoclonal antibodies?

The specific sites on the antibody that interacts with the antigen are