Exam 20: Recombinant Dna Technology

Restriction endonucleases typically recognize palindromic DNA sequences and often generate "sticky ends" or single-stranded DNA overhangs at cut sites.

Some vectors such as pUC18 and others of the pUC series contain a large number of restriction enzyme sites clustered in one region. Which term is given to this advantageous arrangement of restriction sites?

List, in order, the steps usually followed in producing recombinant DNA molecules in a plasmid vector.

What method might be used to study a knockout mouse that, by virtue of the lost gene or genes, generates a lethal condition?

A common term for a plasmid or other DNA element that serves as a cloning vehicle is vector.

If one wishes to clone a gene using typical restriction endonucleases, how does the restriction endonuclease recognize genes in the genome?

Nucleic acid blotting is commonly used in molecular biology. Two types, Southern blots and northern blots, involve gel electrophoresis and a filter, which holds the nucleic acid. Briefly describe the procedure of "blotting" in this context and differentiate between Southern and northern blots.

Name at least two typical characteristics of a DNA cloning plasmid?

When propagating a clone in the lambda phage, would you have more immediate success if the phage entered the lysogenic or the lytic cycle?

A knockout animal, in the context of recombinant DNA technology, is one that has been completely anesthetized.

In general, the main goal of cloning is to include as many different genes as possible in a single cloning vector.

In recombinant DNA technology, YAC, RFLP, and λ have identical uses.

Reverse transcriptase is often used as the heat-stable enzyme in PCR.

Nucleic acid blotting is widely used in recombinant DNA technology. In a Southern blot, one generally ________.

A restriction map provides the location of sites cleaved by restriction enzymes.

In the polymerase chain reaction, what is the purpose of the initial high temperature? What is the purpose of cooling in the second step?

Some restriction endonucleases are capable of producing blunt ends; others can generate "sticky" ends.

Words such as level, civic, and kayak have something in common compared to the fundamental tool of recombinant DNA technology. In the context of recombinant DNA technology, which term would be used to describe such words?

What term is used to refer to the process in which DNA can be introduced into host bacterial cells?

During a PCR, heat is provided to inactivate the polymerase enzyme.