Question 1

The main purpose of a probe is its insertion in plasmid DNA.

Accepted Answer

A) True 
 B)False

Question 2

What is a cDNA molecule?

Accepted Answer

A cDNA mol

Question 3

The function of a ddNTP in DNA sequencing is to methylate guanine.

Accepted Answer

A) True 
 B)False

Question 4

To isolate a bacterium with a plasmid that carries a desired DNA fragment cloned within the ampicillin resistance gene, we should grow bacteria in a medium that contains ampicillin.

Accepted Answer

A) True 
 B)False

Question 5

What might be a reasonable function of restriction endonucleases in a bacterium, distinct from their use by molecular biologists?

Accepted Answer

Isolated from bacteria, restri

Question 6

In recombinant DNA technology, a YAC is an enzyme isolated from a large South American, four-legged mammal.

Accepted Answer

A) True 
 B)False

Question 7

What is the fundamental purpose of creating a knockout organism?

Accepted Answer

The idea is to disrupt or elim

Question 8

In the context of recombinant DNA technology, what is meant by the term vector?

Accepted Answer

A vector is a vehicle to carry

Question 9

Restriction endonucleases are especially useful if they generate "sticky" ends. What makes an end sticky?

Accepted Answer

A)  single-stranded complementary tails 
B)  blunt ends 
C)  poly-A sequences 
D)  5′ cap 
E)  interference 
A)  single-stranded complementary tails 
B)  blunt ends 
C)  poly-A sequences 
D)  5′ cap 
E)  interference

Question 10

One of the primary reasons for generating a large number of clones in a eukaryotic genomic library is that ________.

Accepted Answer

A)  each cosmid replicates in coordination with the host chromosome 
B)  lysogenic phages continue to integrate their DNA into the host chromosome, thus reducing the number of desired recombinant clones 
C)  each vector can take up only a relatively small fraction of the eukaryotic DNA 
D)  each ligation product is sequence specific 
E)  the host range of the vector is limited 
A)  each cosmid replicates in coordination with the host chromosome 
B)  lysogenic phages continue to integrate their DNA into the host chromosome, thus reducing the number of desired recombinant clones 
C)  each vector can take up only a relatively small fraction of the eukaryotic DNA 
D)  each ligation product is sequence specific 
E)  the host range of the vector is limited

Question 11

Of what advantage is it to have a multiple cloning site (multiple unique restriction sites) embedded in the lacZ component of a plasmid?

Accepted Answer

An insert of DNA in the multip

Question 12

The PCR (polymerase chain reaction) protocol that is currently used in laboratories was facilitated by the discovery of a bacterium called Thermus aquaticus in a hot spring inside Yellowstone National Park, in Wyoming. This organism contains a heat-stable form of DNA polymerase known as Taq polymerase, which continues to function even after it has been heated to 95oC. Why would such a heat-stable polymerase be beneficial in PCR?

Accepted Answer

A)  Each cycle includes a "hot" saturation phase (95°
C) , which allows the primers to anneal to the target DNA. 
B)  Each cycle includes a "hot" denaturation phase (95°
C) , which serves to sterilize the culture. 
C)  Each cycle includes a "hot" denaturation phase (95°
C) , which activates the Taq polymerase. 
D)  Each cycle includes a "hot" denaturation phase (95°
C) , which separates the hydrogen bonds that hold the strands of the template DNA together. 
E)  More than one of the above are correct. 
A)  Each cycle includes a "hot" saturation phase (95°
C) , which allows the primers to anneal to the target DNA. 
B)  Each cycle includes a "hot" denaturation phase (95°
C) , which serves to sterilize the culture. 
C)  Each cycle includes a "hot" denaturation phase (95°
C) , which activates the Taq polymerase. 
D)  Each cycle includes a "hot" denaturation phase (95°
C) , which separates the hydrogen bonds that hold the strands of the template DNA together. 
E)  More than one of the above are correct.

Question 13

In the context of molecular genetics, reverse transcription PCR (RT-PCR) refers to ________.

Accepted Answer

A)  assembling a DNA sequence from an mRNA 
B)  assembling an RNA sequence from a DNA sequence 
C)  translating in the 3′ to 5′ direction 
D)  transcribing first, then translating 
E)  making an amino acid sequence from a DNA sequence 
A)  assembling a DNA sequence from an mRNA 
B)  assembling an RNA sequence from a DNA sequence 
C)  translating in the 3′ to 5′ direction 
D)  transcribing first, then translating 
E)  making an amino acid sequence from a DNA sequence

Question 14

E. coli is a common YAC.

Accepted Answer

A) True 
 B)False

The main purpose of a probe is its insertion in plasmid DNA.

What is a cDNA molecule?

The function of a ddNTP in DNA sequencing is to methylate guanine.

To isolate a bacterium with a plasmid that carries a desired DNA fragment cloned within the ampicillin resistance gene, we should grow bacteria in a medium that contains ampicillin.

What might be a reasonable function of restriction endonucleases in a bacterium, distinct from their use by molecular biologists?

In recombinant DNA technology, a YAC is an enzyme isolated from a large South American, four-legged mammal.

What is the fundamental purpose of creating a knockout organism?

In the context of recombinant DNA technology, what is meant by the term vector?

Restriction endonucleases are especially useful if they generate "sticky" ends. What makes an end sticky?

One of the primary reasons for generating a large number of clones in a eukaryotic genomic library is that ________.

Of what advantage is it to have a multiple cloning site (multiple unique restriction sites) embedded in the lacZ component of a plasmid?

In the context of molecular genetics, reverse transcription PCR (RT-PCR) refers to ________.

E. coli is a common YAC.

Introduction to Genetics

Mitosis and Meiosis

Mendelian Genetics

Extensions of Mendelian Genetics

Chromosome Mapping in Eukaryotes

Genetic Analysis and Mapping in Bacteria and Bacteriophages

Sex Determination and Sex Chromosomes

Chromosome Mutations: Variation in Number and Arrangement

Extranuclear Inheritance

DNA Structure and Analysis

DNA Replication and Recombination

DNA Organization in Chromosomes

The Genetic Code and Transcription

Translation and Proteins

Gene Mutation, Dna Repair, and Transposition

Regulation of Gene Expression in Prokaryotes

Regulation of Gene Expression in Eukaryotes

Developmental Genetics

Cancer and Regulation of the Cell Cycle

Genomics, Bioinformatics, and Proteomics

Applications and Ethics of Genetic Engineering and Biotechnology

Quantitative Genetics and Multifactorial Traits

Neurogenetics

Population and Evolutionary Genetics

Filters

Exam 20: Recombinant Dna Technology

The main purpose of a probe is its insertion in plasmid DNA.

What is a cDNA molecule?

The function of a ddNTP in DNA sequencing is to methylate guanine.

To isolate a bacterium with a plasmid that carries a desired DNA fragment cloned within the ampicillin resistance gene, we should grow bacteria in a medium that contains ampicillin.

What might be a reasonable function of restriction endonucleases in a bacterium, distinct from their use by molecular biologists?

In recombinant DNA technology, a YAC is an enzyme isolated from a large South American, four-legged mammal.

What is the fundamental purpose of creating a knockout organism?

In the context of recombinant DNA technology, what is meant by the term vector?

Restriction endonucleases are especially useful if they generate "sticky" ends. What makes an end sticky?

One of the primary reasons for generating a large number of clones in a eukaryotic genomic library is that ________.

Of what advantage is it to have a multiple cloning site (multiple unique restriction sites) embedded in the lacZ component of a plasmid?

In the context of molecular genetics, reverse transcription PCR (RT-PCR) refers to ________.

E. coli is a common YAC.

Introduction to Genetics

Mitosis and Meiosis

Mendelian Genetics

Extensions of Mendelian Genetics

Chromosome Mapping in Eukaryotes

Genetic Analysis and Mapping in Bacteria and Bacteriophages

Sex Determination and Sex Chromosomes

Chromosome Mutations: Variation in Number and Arrangement

Extranuclear Inheritance

DNA Structure and Analysis

DNA Replication and Recombination

DNA Organization in Chromosomes

The Genetic Code and Transcription

Translation and Proteins

Gene Mutation, Dna Repair, and Transposition

Regulation of Gene Expression in Prokaryotes

Regulation of Gene Expression in Eukaryotes

Developmental Genetics

Cancer and Regulation of the Cell Cycle

Genomics, Bioinformatics, and Proteomics

Applications and Ethics of Genetic Engineering and Biotechnology

Quantitative Genetics and Multifactorial Traits

Neurogenetics

Population and Evolutionary Genetics

Filters