Question 1

Chromatin assembly factors (CAFs) move along with the replication fork and assemble new nucleosomes.

Accepted Answer

A) True 
 B)False

Question 2

Bacteria are dependent on telomerase to complete synthesis of their chromosome ends.

Accepted Answer

A) True 
 B)False

Question 3

In the Meselson and Stahl (1958) experiment, bean plants (Vicia faba) were radioactively labeled so that autoradiographs could be made of chromosomes.

Accepted Answer

A) True 
 B)False

Question 4

An endonuclease is involved in removing bases sequentially from one end of DNA or the other.

Accepted Answer

A) True 
 B)False

Question 5

DNA polymerase I is thought to add nucleotides ________.

Accepted Answer

A)  to the 5′ end of the primer 
B)  to the 3′ end of the primer 
C)  in the place of the primer RNA after it is removed 
D)  on single-stranded templates without need for an RNA primer 
E)  in a 5′ to 5′ direction 
A)  to the 5′ end of the primer 
B)  to the 3′ end of the primer 
C)  in the place of the primer RNA after it is removed 
D)  on single-stranded templates without need for an RNA primer 
E)  in a 5′ to 5′ direction

Question 6

As unwinding of the helix occurs during DNA replication, tension is created ahead of the replication fork. Describe the nature of this tension and state the manner in which this tension is resolved.

Accepted Answer

supercoili

Question 7

Which of the following terms accurately describes the replication of DNA in vivo?

Accepted Answer

A)  conservative 
B)  dispersive 
C)  semidiscontinuous 
D)  nonlinear 
E)  nonreciprocal 
A)  conservative 
B)  dispersive 
C)  semidiscontinuous 
D)  nonlinear 
E)  nonreciprocal

Question 8

DNA polymerase III adds nucleotides ________.

Accepted Answer

A)  to the 3′ end of the RNA primer 
B)  to the 5′ end of the RNA primer 
C)  in the place of the primer RNA after it is removed 
D)  to both ends of the RNA primer 
E)  to internal sites in the DNA template 
A)  to the 3′ end of the RNA primer 
B)  to the 5′ end of the RNA primer 
C)  in the place of the primer RNA after it is removed 
D)  to both ends of the RNA primer 
E)  to internal sites in the DNA template

Question 9

DNA replication occurs in the 5′ to 3′ direction; that is, new nucleoside triphosphates are added to the 3′ end.

Accepted Answer

A) True 
 B)False

Question 10

What is the name of the replication unit in prokaryotes, and how does it differ in eukaryotes?

Accepted Answer

replicon; one replic

Question 11

What three possible models were suggested to originally describe the nature of DNA replication?

Accepted Answer

conservati

Question 12

During replication, primase adds a DNA primer to RNA.

Accepted Answer

A) True 
 B)False

Question 13

Assume that you were growing cells in culture and had determined the cell-cycle time to be 24 hours. You introduce 3H thymidine and prepare autoradiographs of metaphase chromosomes after 48 hours. Of the chromosomes that are labeled, you expect two classes: one class that had completed one S phase in the label, and a second class that had completed a cellular division and an additional S phase in the label. Draw the DNA (double-stranded) labeling pattern for each chromosome that you would expect to find in these two types of metaphase chromosomes. (Use a broken line {- - -} for labeled single strands of DNA and a solid line for unlabeled single strands of DNA.)
(a) metaphase chromosome having replicated once in label
(b) metaphase chromosome having gone through two S phases in label

Accepted Answer

Question 14

Which structural circumstance in DNA sets up the requirement for its semidiscontinuous nature of replication?

Accepted Answer

5′ > 3′ polarity restrictions

Question 15

Assume that you are microscopically examining mitotic metaphase cells of an organism with a 2n chromosome number of 4 (one pair metacentric and one pair telocentric). Assume also that the cell passed through one S phase labeling (innermost phosphate of dTTP radioactive) just prior to the period of observation. Assuming that the circle below represents a cell, draw its chromosomes and the autoradiographic pattern you would expect to see.

Accepted Answer

The answer of Assume that you are microscopically examining mitotic...

Question 16

Which protein is responsible for the initial step in unwinding the DNA helix during replication of the bacterial chromosome?

Accepted Answer

The answer of Which protein is responsible for the initial...

Question 17

What primary ingredients, coupled with DNA polymerase I, are needed for the in vitro synthesis of DNA?

Accepted Answer

dNTP, DNA template, primer DNA

Question 18

A characteristic of aging cells is that their telomeres become shorter.

Accepted Answer

A) True 
 B)False

Question 19

The Meselson and Stahl experiment provided conclusive evidence for the semiconservative replication of DNA in E. coli. What pattern of bands would occur in a CsCl gradient for conservative replication?

Accepted Answer

After one generation in the ¹⁴N, there wou

Question 20

Describe the function of the RecA protein.

Accepted Answer

The RecA protein promotes the

Exam 11: DNA Replication and Recombination

Chromatin assembly factors (CAFs) move along with the replication fork and assemble new nucleosomes.

Bacteria are dependent on telomerase to complete synthesis of their chromosome ends.

In the Meselson and Stahl (1958) experiment, bean plants (Vicia faba) were radioactively labeled so that autoradiographs could be made of chromosomes.

An endonuclease is involved in removing bases sequentially from one end of DNA or the other.

DNA polymerase I is thought to add nucleotides ________.

As unwinding of the helix occurs during DNA replication, tension is created ahead of the replication fork. Describe the nature of this tension and state the manner in which this tension is resolved.

Which of the following terms accurately describes the replication of DNA in vivo?

DNA polymerase III adds nucleotides ________.

DNA replication occurs in the 5′ to 3′ direction; that is, new nucleoside triphosphates are added to the 3′ end.

What is the name of the replication unit in prokaryotes, and how does it differ in eukaryotes?

What three possible models were suggested to originally describe the nature of DNA replication?

During replication, primase adds a DNA primer to RNA.

Which structural circumstance in DNA sets up the requirement for its semidiscontinuous nature of replication?

Which protein is responsible for the initial step in unwinding the DNA helix during replication of the bacterial chromosome?

What primary ingredients, coupled with DNA polymerase I, are needed for the in vitro synthesis of DNA?

A characteristic of aging cells is that their telomeres become shorter.

The Meselson and Stahl experiment provided conclusive evidence for the semiconservative replication of DNA in E. coli. What pattern of bands would occur in a CsCl gradient for conservative replication?

Describe the function of the RecA protein.

Introduction to Genetics

Mitosis and Meiosis

Mendelian Genetics

Extensions of Mendelian Genetics

Chromosome Mapping in Eukaryotes

Genetic Analysis and Mapping in Bacteria and Bacteriophages

Sex Determination and Sex Chromosomes

Chromosome Mutations: Variation in Number and Arrangement

Extranuclear Inheritance

DNA Structure and Analysis

DNA Organization in Chromosomes

The Genetic Code and Transcription

Translation and Proteins

Gene Mutation, Dna Repair, and Transposition

Regulation of Gene Expression in Prokaryotes

Regulation of Gene Expression in Eukaryotes

Developmental Genetics

Cancer and Regulation of the Cell Cycle

Recombinant Dna Technology

Genomics, Bioinformatics, and Proteomics

Applications and Ethics of Genetic Engineering and Biotechnology

Quantitative Genetics and Multifactorial Traits

Neurogenetics

Population and Evolutionary Genetics

Filters